#regeneration — Public Fediverse posts
Live and recent posts from across the Fediverse tagged #regeneration, aggregated by home.social.
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https://www.laviezine.com/1634275/regeneration-is-the-new-sustainability-in-japan-tokyo-tatemono-unveils-new-living-lab/ Regeneration is the New Sustainability in Japan: Tokyo Tatemono Unveils New ‘Living Lab’ #Economy&Tech #Editor'sPick #environment #ESG #FutureFoodInstitute #howgood #innovation #regeneraction #regeneration #sustainability #SustainableFood #Tokyo #TokyoLivingLab #UrbanDesign
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https://www.laviezine.com/1634275/regeneration-is-the-new-sustainability-in-japan-tokyo-tatemono-unveils-new-living-lab/ Regeneration is the New Sustainability in Japan: Tokyo Tatemono Unveils New ‘Living Lab’ #Economy&Tech #Editor'sPick #environment #ESG #FutureFoodInstitute #howgood #innovation #regeneraction #regeneration #sustainability #SustainableFood #Tokyo #TokyoLivingLab #UrbanDesign
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Kahneman and Tversky researched the science of error and still made errors.
https://slate.com/technology/2016/12/kahneman-and-tversky-researched-the-science-of-error-and-still-made-errors.html
#commons #uusitalous #p2p #neweconomy #regeneration #dework #cosmolocal
Kahneman and Tversky researche... -
@pressenza.com
Music has always been a messenger and change agent.
Radical which is how jazz may be described, is becoming recognised as a needed new "normal".
#doughnuteconomy #cosmolocalism #regeneration #equality -
Regen Melbourne
I can’t believe I have not written about Regen Melbourne before. I have been loosely involved with them from the beginning and the crafting of their original co-created vision statement, based on Kate Raworth’s Doughnut Economics model. I have also had the pleasure of being part of some of their workshops, and they have also been of great help and support with my own personal regeneration project.
Over the years, Regen Melbourne’s work has steadily evolved and now coalesces around three key Earthshots. An Earthshot is a declaration of intent to solve complex social and ecological challenges. Earthshots recognise that social and ecological regeneration are inseparable. They set an ambition of possibility, coherence of a collective response, and agitation of the conditions we operate within. Earthshots are whole-of-city in scope, long-term in commitment, and open to many entry points.
Each Earthshot has six key qualities.
Wildly Ambitious
Because the scale of the challenges demand it. We are all invested in the social and ecological future of this place.
Systems-oriented
Looking at the conditions we need not just outputs. Oriented towards multi-benefits not single solutions.
Whole-of-city
No one organisation can own the scope of the Earthshot. We are interconnected, so too must our response be.
Coherence, not consensus
There is no blueprint. Forward-leaning action looks like smart collaboration, not waiting for everyone to agree.
Long-term and open
Committed to building broad support over time. An Earthshot is a platform for entry points, not a closed project.
Of and for Melbourne
These Earthshots are in service to place. They build on what makes this city thrive and centre care for our future.
The current Earthshots they are focussing on are:
Swimmable Birrarung
A thriving and swimmable Birrarung, from source to sea
300,000 Streets
A city of participatory and truly liveable streets
Nourished Neighbourhoods
A network of resilient local food economies across our cityYou can find a load more about all of this on their website but what caught my eye recently was this particular article, The Power of Participation: Strengthening the civic muscle of Greater Melbourne. [LINK] The author, Joel Backwell, covers a lot of ground in this post talking about Participatory Urbanism in Melbourne, from “what we are seeing” now, to “where to from here”. For anyone living in Melbourne it’s a great snapshot of some of the wonderful projects and orgs operating on the ground and the work they are doing. The article asks the big questions about participation as well as explaining its importance. Something I have done in previous posts: HERE and HERE
#Biodiversity #Environment #Melbourne #OurWonderfulGreenFuture #Participation #ParticipatoryUrbanism #Regeneration #RegenMelbourne #SolarPunk #StrongTowns #sustainability #Urbanism
“What we are interested in is how all this participation comes together, in service of Greater Melbourne. How intentional must we be in directing this participation towards influencing and shaping power? Or is it enough to just create opportunities for people to come together to discuss what matters to them and their neighbourhoods, and then let a thousand flowers bloom? Can we expect that participation, in response to societal and environmental challenges, will organically scale up to political action? Or will it require organisations like Regen Melbourne to help join the dots, bring these spheres of activity together and build new spaces and institutions for collaboration and collective decision-making at a city scale?“
I’ll wrap this up here because I hope I’ve given enough reasons to go and read the post and check out more about what Regen Melbourne is doing in general on their website here: https://regen.melbourne They have their fingers in a lot of pies, so for anyone interested in Climate Change, Social Justice, or Regeneration, you will find loads of stuff to dive into: Climate Change Exchange – Democracy Beyond the Ballot Box – Connected Corridors – School Streets
Other links
You can follow The REGEN Gazette here: https://regen.melbourne/the-gazette
The People’s Plan for Footscray https://peoplesplanfootscray.net/
McKinnon and Foundations for Tomorrow https://foundationsfortomorrow.org
Doing Co: https://doingco.org/ -
“This cyclic concept of #history results in a strange mixture of resignation and hope. #Resignation - because the crisis of #civilization is inevitable. #Hope - because it is only one phase in the swinging of the #historical #pendulum and will be followed by a period of #regeneration.”
RE: https://bsky.app/profile/did:plc:5zca2ola2zxpkw37w4f3wxtu/post/3mucrapulcc2z -
A volcano of their very own: Mount Elephant
Showers of fiery red, molten volcanic bombs streaking through the air, rivers of flowing lava across the land. This chaotic, noisy and spectacular scene is not Hawaii or Iceland today but Victoria, 180,000 years ago.
At 240 m above sea level the almost perfect cone of Mount Elephant, on Eastern Maar Country at Derrinallum, is one remnant of this explosive time in the state’s history. For millions of years molten rock erupted from over 400 fissures, rifts and cones across Western Victoria, many witnessed and recorded by First Peoples.
Huge volumes of lava flowed across the land. The rock, basalt, was used by settlers to build stone walls and houses, and was crushed for developing roads and farm tracks.
Twenty-five years ago, members of the communities surrounding Mount Elephant rallied to protect this extraordinary place.
“It has a lot of meaning to the community – it really came from the heart. At some of the meetings people were in tears,” says Lesley Brown, Treasurer of the Mount Elephant Committee of Management, volunteers who have been caring for the mountain since it became a Trust for Nature reserve.”
Originally from Melbourne, Lesley moved to Derrinallum to become a dairy farmer with her husband. When Mount Elephant came up for sale in 2000, the community held several meetings to discuss purchasing Mount Elephant. The desire to keep the mountain as part of the community and for recreation was overwhelming, and for Mount Elephant to be restored to its state before European colonisation.
Assisted by a then-Trustee and community member, Trust for Nature formed a partnership to raise $200,000 to purchase Mount Elephant and manage the reserve. Volunteers door-knocked in the local community, a media campaign was launched, and through this $100,000 was raised in five months by the community. Trust for Nature contributed to the balance.
“It was the right thing to do,” Lesley says. “The previous owners wanted it to go to the community. If we hadn’t bought it some other private owners would have.”
The answer involved artistic detective work, using the paintings and sketches of Austrian-born painter Eugene von Guerard. These show that the mountain was likely covered in a woodland of banksias, she oaks, Blackwoods and Manna Gums, with shrubby Sweet Bursaria, Tree Violet and native grasses growing amongst the trees. This is a rare habitat today in Victoria, and much of what remains is degraded. A management plan developed by the Centre for Environmental Management, University of Ballarat, documents the plants and animals that would have lived on Mount Elephant, and how to restore it.
Over the past 25 years volunteers have planted thousands of trees and grasses. Rabbits have been baited and warrens removed. Weeds have been sprayed. The changes have been remarkable: from hundreds and thousands of rabbits, there are now only a handful.
“When we first started there was nothing – it was brown and full of rabbit warrens. Now it’s green and there are lots of trees. People said, ‘You’ll never get rid of the rabbits, you’ll never get rid of the weeds’ – but we have,” says Lesley.
Wildlife is returning, including Wedge-tailed Eagles, wallabies, and kangaroos. This is posing an interesting dilemma: the kangaroos are eating freshly planted trees, which now need to be guarded and fenced. But both are signs that Mount Elephant’s recovery is well on the way.
Walking tracks have been installed, and a visitor center open on Sunday a ernoons, and at other times by request, provides information about this fascinating place and the work that goes into looking after it. Lesley is particularly energised by the people who visit Mount Elephant, including groups of students from local schools and the commitment of the Mount Elephant committee.
“How many people have the opportunity to do what we have done?” says Lesley. “We just get on with the job and then you look back and think ‘The trees are growing, we did that’. It’s a legacy to leave behind for the future, so that future generations can continue to enjoy this place.”
This article has been entirely reproduced from the original here: https://trustfornature.org.au/a-volcano-of-their-very-own-mount-elephant/
Check out the Trust For Nature website and see some of the other great work they are doing all over Australia.
And perhaps even consider signing up to help support their work.- A volcano of their very own: Mount Elephant
- Unique conservation course for First Peoples celebrates 10 years
- Celebrating the caretakers of Ralph Illidge Sanctuary
- Victorian landholders to lead massive conservation effort
- Haven of abundance
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Bacteria infecting the #intestine are recognized by epithelial and immune cells. This study shows that adult #Drosophila midgut intestinal stem cells recognize pathogenic bacteria via the PGRP proteins, promoting #regeneration.
https://plos.io/3SuoWnG -
Climate-saving wonder – or €240m vanity bauble that ages like milk? The rocks set to hit Rotterdam docks
By Catherine SlessorMVRDV – the firm behind Marble Arch Mound – says Rotterdam ROCKS! will inspire visitors to live sustainably. But in an era when the greenest building is the one that already exists, this tottering cairn looks set to misfire
https://www.theguardian.com/artanddesign/2026/aug/25/rotterdam-rocks-mvrdv
#Architecture #Artanddesign #Culture #Netherlands #Climatecrisis #Regeneration #Environment #TheGuardian #CatherineSlessor
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Climate-saving wonder – or €240m vanity bauble that ages like milk? The rocks set to hit Rotterdam docks https://www.theguardian.com/artanddesign/2026/aug/25/rotterdam-rocks-mvrdv #Architecture #ArtAndDesign #Culture #Netherlands #ClimateCrisis #Regeneration #Environment
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The Reason Many People Don’t Volunteer (It’s Not Time)
Last week I was sat in a room filled with a load of really passionate people who had come together to discuss creating a Community Newspaper. The ideas were flowing. The questions were flying about and the enthusiasm was there. But when it came to the crunch; “Hands up who wants to be part of the initial steering committee?” all that momentum came crashing to a halt. The hands did not come up.
It’s easy to think “Nobody has the time” and that’s the problem, but there is a bigger issue that holds people back. People don’t like to let other people down. Not wanting to let people down is perhaps a bigger barrier to getting people to volunteer than time.
This morning I got a very similar response talking to a gentleman about a regeneration project in my local park. He was initially reluctant to sign up because he just didn’t know if he could commit the time needed to the project. When I explained that he wasn’t obligated to come to every event and he could pick the ones that suited him, he was happy to sign up.
This is not a new observation to anyone working in this field. It’s called “commitment anxiety” or “anticipatory guilt.”: the fear that saying yes means taking responsibility for others’ expectations. When people worry about letting down their neighbours, friends, or community members who are counting on them, the social cost of failing outweighs the benefit of contributing.
Image via The Tampa Bay Estuary Program on UnsplashOn top of this there are a few other dimensions to this problem.
Social Risk vs. Practical Constraint: The “no time” explanation is socially safer. It’s concrete and feels less personal. Admitting fear of disappointing others would mean admitting vulnerability. So “time” becomes the convenient public reason while the real barrier sits beneath the surface.
Diffused Responsibility Paradox: Ironically, the stronger the group enthusiasm, the harder it can be for individuals to commit. When everyone seems passionate and capable, each person may think “someone else will take this lead.” The momentum of collective excitement can actually suppress individual action because nobody wants to stand out or fail publicly. This feels very much like what I experienced in the Community Newspaper meeting.
Perfectionism in Community Projects: Community initiatives carry emotional weight beyond transactional tasks. People worry they’ll damage relationships if the project struggles, not just that a deadline might be missed. That stakes-raising makes the ask more daunting than a typical volunteer opportunity.
So, is there anything we can learn from this? Are there any tips or tricks to get people to volunteer that sidestep these issues?
Small, time-bound trial commitments: EG “It’s just for 6 weeks, then we reassess” This lowers the perceived risk and gives people a really clear escape route, after 6 weeks. And then they can say “I don’t have time…” Instead of “join the steering committee,” try “come to one planning session next Thursday.” Each micro-commitment builds identity alignment and people start seeing themselves as someone who shows up. Once that self-image forms, larger commitments feel like a natural extension rather than a leap.
Making roles modular rather than monolithic: Let’s people contribute pieces without owning outcomes. Let’s people decide what they do, and when, so they feel like they are in control. Instead of giving people a task, ask them “What do you want to do?” and invite them to commit on their own terms. Also in an established organisation you can create a Shadowing Option so people can understand the scope before committing, offering a “shadow” role first.
Explicitly naming the fear: “I know saying yes feels daunting right now but…” This can reduce its power. Make it clear that quitting in 12 weeks is better than contributing nothing at all. Normalise exit on-ramps. Make leaving frictionless and guilt-free from the outset. Something like: “Anyone who joins the steering committee can step back at any point, no explanation needed.” When people know the door swings open both ways, commitment feels less like a trap. The irony is that making it easy to leave often makes people stay longer.
#Charity #Environment #OurWonderfulGreenFuture #Regeneration #sustainability #Volunteering
So that’s it. I wish I had all that in my head last week! For my Regen project simply getting people onto a mailing list is the big thing. Allowing people to passively sit in the background as potential volunteers until the day comes when they are in a position to volunteer pays off. -
RE: https://scicomm.xyz/@mustapipa/117137230074004940
If economics can be said to speak, it needs to shout LOUDLY about proper green regeneration (rather than plant-a-tree make-work): “Scientists found that natural #forest regeneration delivered substantial carbon and #biodiversity benefits and could be up to 96% cheaper than traditional tree-planting programs.” 🌳🌲🌴🎄
Fabulous news - thanks so much for sharing! 😀🙏🏻
#regeneration #forestRegeneration #costBenefit #carbonSink #RoI
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Astrozyten könnten sich nach Hirnverletzungen gezielt erneuern: Forschende der Universität Zürich beobachteten bei Mäusen, wie neue Zellkerne entlang regenerativer Astrozyten-Fortsätze in die geschädigte Region wandern. 🧠🔬 Fortschritt im Verständnis von Reparaturmechanismen—und potenziell neue Therapieansätze. #Neurowissenschaften #Hirnforschung #Regeneration #Medizin #NatureNeuroscience https://www.nau.ch/news/forschung/astrozyten-erneuern-geschadigtes-hirngewebe-67162323
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Wow, at the beginning of Destiny of the Daleks Part 1, Romana is trying on different bodies for her #Regeneration. Though the brunette form she had in the previous episode is much more interesting 😺 So, yes, apparently I'm surveying all of the companions of #ClassicWho as part of my own cycle.
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Hope for a wilder Britain with George Monbiot.
"For me, the driver has always been love. It's been a love of thriving nature, it's been a love of rich and interesting landscapes and it's been this despair at seeing so few of them in our own country."Reading George Monbiot's book 'Feral' a decade ago opened Ben Goldsmith's eyes to the truly dire state of nature in #Britain, and to the notion that #rewilding.
#Nature #Regeneration
https://pocketcasts.com/podcast/rewilding-the-world-with-ben-goldsmith/ede9e000-c766-013b-f42e-0acc26574db2/hope-for-a-wilder-britain-with-george-monbiot/adcedc44-5aa9-4dfc-9ac3-c4758355ca0f -
You can now access all replays of the International Festival Of Wild & Kind Ideas from May 2026 - hosted by #MoragGamble - in exchange for your email and an optional financial contribution:
https://permacultureeducationinstitute.org/festival-2026/
Soo many important & inspiring talks, people and ideas ✨🌱
#rewilding #permaculture #ecology #kindness #wilderness #future #regeneration #culture #humanity
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Added a mini-gallery of our interdisciplinary work for water here: https://natasonni.me/vodama
#art #mastoart #science #water #nature #rewilding #river #sustainability #regeneration
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Life on the 'worst street' in Anfield where people 'keep their blinds shut'
https://www.liverpoolecho.co.uk/news/liverpool-news/life-worst-street-anfield-people-34386070
People living next to empty land told the ECHO they are in "dire straits" #LFC #YNWA #LiverpoolCouncil #Regeneration #LiverpoolFC #Anfield -
Sacred Sites As Refuges For Native Plant Diversity In An Urbanized Tropical Landscape [Madagascar]
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https://doi.org/10.1038/s44284-026-00489-x <-- shared paper
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H/T @nicolas Salliou | Senior scientist at ETH Zürich
“When [the authors] investigated the potential for blue-green infrastructures for the capital of Madagascar,… a large team surveyed plant communities along a rural-urban gradient.
They found that 12 Malagasy "sacred" hill settlements, situated in the core of a pre-colonial kingdom, are particularly precious as they host a high diversity of native plants. A key insights when one considers that Madagascar is a unique hotspot for biodiversity and human cultures…”
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“In fragmented and intensively managed remnant urban green spaces, enabling natural regeneration is crucial to sustain ecosystem functioning and associated services. Natural regeneration therefore offers an important lens into ecological resilience in human-modified landscapes. Here [the authors] present one of the most comprehensive field-based assessments of urban plant diversity in the Afrotropics, comprising over 25,000 naturally regenerating woody plant individuals of 206 species (39% native) across 329 vegetation patches in Antananarivo, Madagascar. Landscape-level native species diversity declined with greater building complexity, density and socio-economic development, whereas exotic species showed no consistent associations with determinants of urban characteristics. Native species richness was highest in ‘sacred’ forest fragments, many of which contain historic remains dating back to pre-colonial times. Notably, seven of the ten most species-rich patches were located in such sites. [Their] findings highlight the potentially important role of these sites in sustaining native species diversity and indicate how socio-cultural factors can present both opportunities and barriers for tropical urban conservation and restoration...”
#sacredsites #antananarivo #madagascar #vegetation #nativeplant #urban #greenspace #tropical #hillsettlements #spatialanalysis #survey #ecosystem #biodiversity #ecology #regeneration #humanimpacts #GIS #spatial #mapping #afrotropics #forest #forestfragments #precolonial #sustainability #diversity #native #sociocultural #restoration -
How Elephant and Castle risks becoming London’s gentrification ‘patient zero’
By Phillip Inman and Sarah ButlerNew £500m town centre welcomes its first tenant this month, but displaced traders say promises that they could return have been broken
#Gentrification #London #Hospitalityindustry #Retailindustry #Housing #Regeneration #Communities #Society #UKnews #Cities #Business #TheGuardian #PhillipInman #SarahButler
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Severed #seacucumber appendages don't seem to die
Scientists have discovered bits of tissue removed from a species of sea cucumber called Psolus fabricii can keep on living indefinitely if they’re left in ordinary seawater.
While sea cucumbers can easily regrow these legs and arms, they don’t have whole-body #regeneration like flatworms and some starfish do. Their severed bits don’t grow into new sea cucumbers. But it turns out they don’t die, either.
https://arstechnica.com/science/2026/05/severed-sea-cucumber-appendages-dont-seem-to-die/ -
Do you think hemp could help solve some of today's challenges? 🤔🌿
From nutritious food and natural fibres to building materials, skincare, wellness products and healthier soils, hemp is one of nature's most versatile crops.
It won't solve everything on its own—but it's a powerful part of a more regenerative future.
What do you think?
#ClimateAction #OrganicHemp #Farmlife #Regeneration #LandJustice
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#FollowTheMoney 🧵 98/n In my attempts to launch #RegenerativeAnthropology a bit, i am interacting with so many amazing, creative, brilliant people working in #Regeneration. It is nourishing, exciting, inspiring. But all of us, all of us, are scrambling around for sources of funding all the time. So little money in these spaces! This has made me think again about how money doesn’t go towards life, it goes towards death, dead things.
Or, to put it differently: -
This morning, we had the privilege of educating young people on proper waste management and environmental responsibility. By empowering the next generation with sustainable habits, we are building cleaner communities and protecting our environment for the future. Every lesson shared today is a step toward lasting positive change.
#climate #environment #ecosystem #climatechange #nature #conservation #biodiversity #regeneration #ecology #afforestation #wastemanagement #sanitation #climatecrisis -
We call them "invasive." Nature calls them survivors. Before we wage war on a plant, let's ask what pain the land is trying to reveal. A wounded Earth sends messengers, not monsters. Heal the soil, restore biodiversity, and life will find its balance again. Don't fight nature—listen to it. 🌍💚 #Regeneration #SoilHealth #Ecology #ClimateAction #Europe #Nature #Permaculture
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We call them "invasive." Nature calls them survivors. Before we wage war on a plant, let's ask what pain the land is trying to reveal. A wounded Earth sends messengers, not monsters. Heal the soil, restore biodiversity, and life will find its balance again. Don't fight nature—listen to it. 🌍💚 #Regeneration #SoilHealth #Ecology #ClimateAction #Europe #Nature #Permaculture
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‘The environmental movement needs many hands’: saving Australia’s biodiversity is getting personal
Powered by individual donations and bequests, the country now has one of the world’s largest networks of privately protected and managed land
by James Norman, Fri 17 Apr 2026
Excerpt: "Over three decades later, #BushHeritage today owns and protects 45 bought or gifted bush reserves around #Australia – covering more than 1.4m hectares of land and providing habitat for over 9,000 native species. Working with #FirstNations communities and farmers, it supports the management of an additional 20.5m hectares beyond what it owns directly.
" 'Some of Australia’s most threatened and fragile #ecosystems and #wildlife sit outside of the national reserve system,' says Rachel Lowry, Bush Heritage Australia’s chief executive officer.
" 'They’re found on private land or pastoral country, and they may have deep cultural and ecological values, but they’re not protected from threats such as #LandClearing, #mining or #InvasiveSpecies.'
"The organisation reports a rise in the number of personal bequests, from 2,500 in 2022 to more than 4,600 bequests last year. Lowry puts the increase down to a personal desire to have a meaningful impact on the worsening global climate and #biodiversity crisis.
"Bush Heritage’s model has inspired other groups around Australia to acquire and conserve lands and forests of significant ecological value. One such example is the #NorthEastTasmania #LandTrust (#NETLT), which secretary Dr Christine Hosking describes as 'a microcosm of Bush Heritage'.
"Formed in 2009, the organisation manages donated land through weed control and regeneration of the native environment.
" 'Currently, we have 10 acres [4 hectares] of endangered eucalyptus forest in #BinalongBay that adjoins the #BayOfFires #Conservation Area, and a smaller patch of coastal habitat for the endangered swift parrot,' she says."
#SolarPunkSunday #LandConservation #EndangeredSpecies #ClimateCrisis #ProtectTheForest #ProtectTheForests #Regeneration #PreserveNature #Conservationists #IndigenousAustralians #TraditionalLandManagement
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Spirituality & Religious Studies @spiritualityreligiousstudies.wordpress.com@spiritualityreligiousstudies.wordpress.com ·Eternal Security
This is also known as “once saved, always saved.” It’s the belief giving Christian believers with an absolute assurance of their final salvation.
Its development, especially in Protestantism, has given rise to a plethora of different interpretations. Especially when defining aspects of determinism, libertarian free will & the significance of personal perseverance.
In the early 5th century, Augustinian soteriology views of predestination by predetermination came about, they didn’t validate Eternal Security. Soteriology is the doctrine of salvation.
By the 16th century, this idea became meshed into the theology of John Calvin & other reformers. Calvinist circles initially embraced Eternal Security under the name of “perseverance of the saints.” Eventually, the name became a synonym of the Calvinist doctrine of perseverance independent of its practical interpretations.
Then in the early 20th century, Eternal Security started to become a defining doctrine of Southern Baptist traditionalism. Around the same time, it also became a part of Plymouth Brethren theology. In this, the 2 forms represents its main form today.
In the 1980s, the Free Grace movement voiced that Eternal Grace was independent of the idea of the “Hyper-Grace” idea.
Eternal Security is based on the faith that the believer is an elect by divine determination. This is in Calvinist circles & has the minority worldview.
In some non-Calvinist circles & the prevailing worldview, Eternal Security is based on the faith that regeneration leads to unconditional perseverance & then salvation.
In other non-Calvinist circles & currently growing in the Free Grace views, Eternal Security is based on the faith that regeneration leads to salvation independent of perseverance.
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#16thCentury #1980s #5thCentury #Augustinians #Calvinist #Christian #Determinism #DivineDetermination #Early20thCentury #EternalSecurity #FreeGraceMovement #HyperGrace #JohnCalvin #LibertarianFreeWill #Perseverance #PerseveranceOfTheSaints #PlymouthBrethen #Predestination #Protestantism #Reformers #Regeneration #Salvation #Soteriology #SouthernBaptist #SouthernBaptistTraditionalism
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Michael Cieply: Two Weeks Out, The Academy Museum Preps A Cautious But Correct ‘Hollywoodland’ Opening
#Commentary #News #AcademyMuseumOfMotionPictures #Antisemitism #BillKramer #DaraJaffe #Gaza #Hollywoodland #JacquelineStewart #Jews #JohnWaters #MichaelCieply #NealGabler #ReGeneration -
Hey fediverse,
We're hosting our #IBID open access #conference again (free admission) at our venue from17-19 October 2023. It is an international interdisciplinary conference on #cancer #biology & #diagnostics, #toxicology, #bioanalytics, #bioinformatics, in vitro test systems, #POCT, #neurodegenerative #diseases, #ageing, #immunology and #regeneration. Here is the current link:
Much is still under construction.
Feel free to share my toot. -
Wird von der Leber ein Teil entfernt, kann der Körper fehlendes Gewebe nachwachsen lassen. Womöglich hängt diese Regeneration auch von den vorhandenen Bakterien im Darm ab.
Darmbakterien können Leberzellwachstum beeinflussen
#Leber #Leberzelle #Regeneration #Transplantation #Lebertransplantation #Darmbakterien #Mikrobiom #Medizin -
CW: Using Human Pluripotent Stem Cells to Create Human Skeletal Muscle Organoids for Repair and Regeneration
Skeletal #muscle is a type of tissue that makes up a large part of the human body. It is made up of many different cells that are able to contract and move. Skeletal muscle has the ability to #repair itself when it is damaged due to #aging, exercise, or diseases like #MuscularDystrophy. A small group of cells called #SatelliteCell s help with the repair process. Scientists have been trying to create models to study how #Skeletalmuscle develops and regenerates. Recently, they have been using human pluripotent #StemCell to create 3D models of skeletal muscle tissue. However, these models have not been able to recreate the full process of muscle regeneration. In this research paper, the authors introduce a new method of using human pluripotent stem cells to create 3D models of skeletal muscle tissue that can retain the ability to repair itself.
Over the past decades, scientists have used #animalmodel to study #muscleregeneration, which is regulated by #stemcell s. These animal models have been very helpful in understanding the mechanisms of muscle #regeneration, but they don't always accurately reflect the same range of diseases that humans experience. Therefore, researchers have suggested creating reliable in vitro models using human muscle cells. ( #hPSC s) could be used to create 3D human skeletal muscle #organoid s ( #hSkMO s) that contain sustainable #stemcell and distinct myofibers with the same proteins and structure as adult muscles. Previous approaches to skeletal muscle differentiation have been developed using 2D #culture systems, but these lack the natural environment and #StemCell niche that are necessary to model adult #myogenesis and muscle #regeneration.
#Stemcell s ( #SC s) can be used to repair damaged muscle tissue. They explain that SCs can be activated in response to muscle injuries and that other #cell types can contribute to the process of #myogenesis. The author then goes on to explain that #cytokine s, such as IL-4, can influence the #InflammatorySystem and promote SCs differentiation, which helps with muscle regeneration. While #organoid s generated from #hPSC s have potential, they do not fully replicate the in vivo native microenvironment. To address this, treat the #hSkMO s with extrinsic #cytokine s to promote #muscle #regeneration . #hSkMO s might then be used to study aspects of human muscle #biology and to identify novel #therapeutic candidates for muscle-wasting disorders.
To create a 3D structure of muscle tissue. They used #WNT activator and #BMP inhibitors at the beginning of the differentiation process to induce paraxial #mesodermal #cell s. They then added #FGF2 to the Matrigel to promote the 3D structure. #HGF and IGF1 were added later to accelerate the #myogenic specification and further #myofiber differentiation. They optimized the timing of the Matrigel embedding to day seven. After this, they observed #neuralcell s and withdrew FGF2 to focus on muscle tissue development. They then prolonged the HGF and IGF1 treatment to propagate #myogenic #progenitor s. They found that 62% of the #tissue was #skeletalmuscle tissue and that it contained PAX7+ #myogenic #stem / #progenitor cells, MYOD+ activated/committed #myoblast s, and MYOG+ #myocyte s. They also found that 31% of PAX7+/Ki67− and 29% of MYOD−/PAX7+ non-dividing quiescent SCs were present in the mature #hSkMO s. This indicates that the #hSkMO s were able to effectively recreate #embryo nic #myogenesis and have regenerative potential. Future studies using #singlecell #RNA sequencing may be necessary to further characterize the different types of cells in #hSkMO s.
The stepwise process to generate human skeletal muscle organoid s (hSkMOs) from human pluripotent stem cells (hPSCs)
The process begins with dissociating #hPSC s into #singlecell s and allowing them to form #embryoid bodies ( #EB s) in low-attachment V-shaped 96-well plates. Then, paraxial #mesodermal differentiation is promoted with #WNT activation, BMP inhibition, and FGF2 signaling. The expression of pluripotency markers OCT4 and NANOG decreases, and the expression of #mesoderm markers Brachyury, T-Box transcription factor 6 (TBX6), and mesogenin 1 (MSGN1) increases. To further characterize paraxial #mesoderm al differentiation, TBX6 is #immunostain ed. After paraxial #mesodermal induction, the #organoid s are embedded with growth factor-reduced Matrigel and transferred to a six-well plate on an orbital shaker. Growth factors are then added to the #myogenic specification media, and #hSkMO s are cultured until the day of analysis. The orbital shaker improves the viability, survival, and differentiation of hSkMOs by increasing the penetration rate of oxygen and nutrients into the core area of hSkMOs. The #hSkMOs gradually grow to more than 1.5 mm in diameter by day 60, appearing round-shaped, uniformly sized, and having relatively homogenous morphology. PAX3 and PAX7 are #myogenic progenitor markers, and their expression is verified by qRT-PCR and #cryo sections. The #myogenic cells appear as clusters, and approximately 9% of PAX7+ cells are double-positive for Ki67 at day 30, demonstrating that proliferating cells are #myogenic #progenitor s in hSkMOs. This indicates that the in vitro #hSkMO #culturesystem is able to recapitulate the features of embryonic skeletal #muscle development.
The different types of #SkeletalMuscle stem/progenitor cells that are involved in myogenesis, the process of muscle formation.
The researchers used qRT-PCR analysis and #immunohistochemistry to identify and characterize the different types of cells. They found that PAX3 and PAX7 (SC markers) were the major population during the early stage of #myogenesis, and that MYOD (proliferating and activated SC marker) and MYOG (differentiated myocyte marker) increased over time. They also observed that MYOD−/PAX7+, MYOD+/PAX7+, and MYOD+/Ki67+ cells accounted for 29%, 6%, and 8% of the putative quiescent, activated, and proliferating #SC s, respectively. MYOD+/PAX7− cells constituted 39% of differentiating myoblasts, and MYOG−/PAX7+ cells constituted 23% of putative quiescent SCs. MYOG+/PAX7− cells accounted for 30% of differentiated #myocyte s, and 8% and 6% of the MYOG+ cells in #hSkMO s co-expressed PAX7 and Ki67, respectively. This data shows that the researchers were able to identify and characterize different types of skeletal muscle stem/progenitor cells during #myogenesis.
The text is discussing the results of a research study that used hSkMOs (human skeletal muscle #organoid s) to study the development of skeletal muscle #tissue. The study found that the #hSkMO s grew exponentially in size within two months, and the growth rate then steadily decreased. The researchers then used scanning electron microscopy (SEM) imaging and confocal microscopy to examine the cytoarchitecture of the hSkMOs. They found that the hSkMOs contained a large population of terminally differentiated #myogenic cells and a small population of preserved myogenic stem/progenitor cells. They also found that the hSkMOs contained a substantial proportion of TITIN+ muscle cells and MAP2-positive #neuron s. To further characterize the presence of sustainable stem cells within the mature hSkMOs, they quantified the amount of dormant stem cells by #confocal #microscopy imaging. The results showed that approximately 56%, 31%, and 5% of PAX7+/Ki67- putative dormant stem cells existed throughout the differentiation of hSkMOs at days 30, 70, and 130, respectively. This indicates that the hSkMOs contained mature skeletal muscle properties and had the potential for #regeneration .
The researchers wanted to see if the #hSkMO s (human #skeletal muscle #organoid s) had the ability to regenerate #muscle #tissue after damage. To test this, they treated the hSkMOs with a cardiotoxin (CTX) which is known to induce muscle inflammation and damage. They then observed a decrease in PAX7+ and MYOD+ cells in the hSkMOs. To further test the #regenerative potential of the #hSkMO s, they added interleukin-4 (IL-4) to the medium to promote #muscleregeneration. After 14 days, they observed a significant increase in MYOG+ myocytes in the CTX-injured hSkMOs with the treatment of IL-4 compared to the CTX-injured hSkMOs without the treatment. This suggests that the hSkMOs have the potential to regenerate muscle tissue after damage.
Generation of Skeletal Muscle Organoids from Human Pluripotent Stem Cells to Model Myogenesis and Muscle Regeneration
Authors :
Min-Kyoung Shin , Jin Seok Bang , Jeoung Eun Lee , Hoang-Dai Tran , Genehong Park , Dong Ryul Lee and Junghyun Jo
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Same with #antiBacterial waterTreatments like #Dettol.
Generally speaking, due to #govtProtections one doesn't need to add products to tapWater in most if not all Australian cities to wash an open #wound to prevent #infection. TapWater will do the job.
In fact we've seen adding too much antibacterial can damage #skin around the wound and retard normal #regeneration / repair.
Whether the #water is drinkable is another story.