#regeneration — Public Fediverse posts
Live and recent posts from across the Fediverse tagged #regeneration, aggregated by home.social.
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Kahneman and Tversky researched the science of error and still made errors.
https://slate.com/technology/2016/12/kahneman-and-tversky-researched-the-science-of-error-and-still-made-errors.html
#commons #uusitalous #p2p #neweconomy #regeneration #dework #cosmolocal
Kahneman and Tversky researche... -
Liscard Dominick House Update | Wirral View
Plans to deal with an eyesore building in Liscard town centre are set to be decided upon by…
#Liverpool #UnitedKingdom #UK #GB #England #Headlines #News #Europe #EU #Britain #GreatBritain #Regeneration
https://www.europesays.com/uk/1186234/ -
https://www.europesays.com/uk/1186234/ Liscard Dominick House Update | Wirral View #Britain #England #GreatBritain #Liverpool #Regeneration #UK #UnitedKingdom
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Regen Melbourne
I can’t believe I have not written about Regen Melbourne before. I have been loosely involved with them from the beginning and the crafting of their original co-created vision statement, based on Kate Raworth’s Doughnut Economics model. I have also had the pleasure of being part of some of their workshops, and they have also been of great help and support with my own personal regeneration project.
Over the years, Regen Melbourne’s work has steadily evolved and now coalesces around three key Earthshots. An Earthshot is a declaration of intent to solve complex social and ecological challenges. Earthshots recognise that social and ecological regeneration are inseparable. They set an ambition of possibility, coherence of a collective response, and agitation of the conditions we operate within. Earthshots are whole-of-city in scope, long-term in commitment, and open to many entry points.
Each Earthshot has six key qualities.
Wildly Ambitious
Because the scale of the challenges demand it. We are all invested in the social and ecological future of this place.
Systems-oriented
Looking at the conditions we need not just outputs. Oriented towards multi-benefits not single solutions.
Whole-of-city
No one organisation can own the scope of the Earthshot. We are interconnected, so too must our response be.
Coherence, not consensus
There is no blueprint. Forward-leaning action looks like smart collaboration, not waiting for everyone to agree.
Long-term and open
Committed to building broad support over time. An Earthshot is a platform for entry points, not a closed project.
Of and for Melbourne
These Earthshots are in service to place. They build on what makes this city thrive and centre care for our future.
The current Earthshots they are focussing on are:
Swimmable Birrarung
A thriving and swimmable Birrarung, from source to sea
300,000 Streets
A city of participatory and truly liveable streets
Nourished Neighbourhoods
A network of resilient local food economies across our cityYou can find a load more about all of this on their website but what caught my eye recently was this particular article, The Power of Participation: Strengthening the civic muscle of Greater Melbourne. [LINK] The author, Joel Backwell, covers a lot of ground in this post talking about Participatory Urbanism in Melbourne, from “what we are seeing” now, to “where to from here”. For anyone living in Melbourne it’s a great snapshot of some of the wonderful projects and orgs operating on the ground and the work they are doing. The article asks the big questions about participation as well as explaining its importance. Something I have done in previous posts: HERE and HERE
#Biodiversity #Environment #Melbourne #OurWonderfulGreenFuture #Participation #ParticipatoryUrbanism #Regeneration #RegenMelbourne #SolarPunk #StrongTowns #sustainability #Urbanism
“What we are interested in is how all this participation comes together, in service of Greater Melbourne. How intentional must we be in directing this participation towards influencing and shaping power? Or is it enough to just create opportunities for people to come together to discuss what matters to them and their neighbourhoods, and then let a thousand flowers bloom? Can we expect that participation, in response to societal and environmental challenges, will organically scale up to political action? Or will it require organisations like Regen Melbourne to help join the dots, bring these spheres of activity together and build new spaces and institutions for collaboration and collective decision-making at a city scale?“
I’ll wrap this up here because I hope I’ve given enough reasons to go and read the post and check out more about what Regen Melbourne is doing in general on their website here: https://regen.melbourne They have their fingers in a lot of pies, so for anyone interested in Climate Change, Social Justice, or Regeneration, you will find loads of stuff to dive into: Climate Change Exchange – Democracy Beyond the Ballot Box – Connected Corridors – School Streets
Other links
You can follow The REGEN Gazette here: https://regen.melbourne/the-gazette
The People’s Plan for Footscray https://peoplesplanfootscray.net/
McKinnon and Foundations for Tomorrow https://foundationsfortomorrow.org
Doing Co: https://doingco.org/ -
Bacteria infecting the #intestine are recognized by epithelial and immune cells. This study shows that adult #Drosophila midgut intestinal stem cells recognize pathogenic bacteria via the PGRP proteins, promoting #regeneration.
https://plos.io/3SuoWnG -
The Reason Many People Don’t Volunteer (It’s Not Time)
Last week I was sat in a room filled with a load of really passionate people who had come together to discuss creating a Community Newspaper. The ideas were flowing. The questions were flying about and the enthusiasm was there. But when it came to the crunch; “Hands up who wants to be part of the initial steering committee?” all that momentum came crashing to a halt. The hands did not come up.
It’s easy to think “Nobody has the time” and that’s the problem, but there is a bigger issue that holds people back. People don’t like to let other people down. Not wanting to let people down is perhaps a bigger barrier to getting people to volunteer than time.
This morning I got a very similar response talking to a gentleman about a regeneration project in my local park. He was initially reluctant to sign up because he just didn’t know if he could commit the time needed to the project. When I explained that he wasn’t obligated to come to every event and he could pick the ones that suited him, he was happy to sign up.
This is not a new observation to anyone working in this field. It’s called “commitment anxiety” or “anticipatory guilt.”: the fear that saying yes means taking responsibility for others’ expectations. When people worry about letting down their neighbours, friends, or community members who are counting on them, the social cost of failing outweighs the benefit of contributing.
Image via The Tampa Bay Estuary Program on UnsplashOn top of this there are a few other dimensions to this problem.
Social Risk vs. Practical Constraint: The “no time” explanation is socially safer. It’s concrete and feels less personal. Admitting fear of disappointing others would mean admitting vulnerability. So “time” becomes the convenient public reason while the real barrier sits beneath the surface.
Diffused Responsibility Paradox: Ironically, the stronger the group enthusiasm, the harder it can be for individuals to commit. When everyone seems passionate and capable, each person may think “someone else will take this lead.” The momentum of collective excitement can actually suppress individual action because nobody wants to stand out or fail publicly. This feels very much like what I experienced in the Community Newspaper meeting.
Perfectionism in Community Projects: Community initiatives carry emotional weight beyond transactional tasks. People worry they’ll damage relationships if the project struggles, not just that a deadline might be missed. That stakes-raising makes the ask more daunting than a typical volunteer opportunity.
So, is there anything we can learn from this? Are there any tips or tricks to get people to volunteer that sidestep these issues?
Small, time-bound trial commitments: EG “It’s just for 6 weeks, then we reassess” This lowers the perceived risk and gives people a really clear escape route, after 6 weeks. And then they can say “I don’t have time…” Instead of “join the steering committee,” try “come to one planning session next Thursday.” Each micro-commitment builds identity alignment and people start seeing themselves as someone who shows up. Once that self-image forms, larger commitments feel like a natural extension rather than a leap.
Making roles modular rather than monolithic: Let’s people contribute pieces without owning outcomes. Let’s people decide what they do, and when, so they feel like they are in control. Instead of giving people a task, ask them “What do you want to do?” and invite them to commit on their own terms. Also in an established organisation you can create a Shadowing Option so people can understand the scope before committing, offering a “shadow” role first.
Explicitly naming the fear: “I know saying yes feels daunting right now but…” This can reduce its power. Make it clear that quitting in 12 weeks is better than contributing nothing at all. Normalise exit on-ramps. Make leaving frictionless and guilt-free from the outset. Something like: “Anyone who joins the steering committee can step back at any point, no explanation needed.” When people know the door swings open both ways, commitment feels less like a trap. The irony is that making it easy to leave often makes people stay longer.
#Charity #Environment #OurWonderfulGreenFuture #Regeneration #sustainability #Volunteering
So that’s it. I wish I had all that in my head last week! For my Regen project simply getting people onto a mailing list is the big thing. Allowing people to passively sit in the background as potential volunteers until the day comes when they are in a position to volunteer pays off. -
Sacred Sites As Refuges For Native Plant Diversity In An Urbanized Tropical Landscape [Madagascar]
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https://doi.org/10.1038/s44284-026-00489-x <-- shared paper
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H/T @nicolas Salliou | Senior scientist at ETH Zürich
“When [the authors] investigated the potential for blue-green infrastructures for the capital of Madagascar,… a large team surveyed plant communities along a rural-urban gradient.
They found that 12 Malagasy "sacred" hill settlements, situated in the core of a pre-colonial kingdom, are particularly precious as they host a high diversity of native plants. A key insights when one considers that Madagascar is a unique hotspot for biodiversity and human cultures…”
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“In fragmented and intensively managed remnant urban green spaces, enabling natural regeneration is crucial to sustain ecosystem functioning and associated services. Natural regeneration therefore offers an important lens into ecological resilience in human-modified landscapes. Here [the authors] present one of the most comprehensive field-based assessments of urban plant diversity in the Afrotropics, comprising over 25,000 naturally regenerating woody plant individuals of 206 species (39% native) across 329 vegetation patches in Antananarivo, Madagascar. Landscape-level native species diversity declined with greater building complexity, density and socio-economic development, whereas exotic species showed no consistent associations with determinants of urban characteristics. Native species richness was highest in ‘sacred’ forest fragments, many of which contain historic remains dating back to pre-colonial times. Notably, seven of the ten most species-rich patches were located in such sites. [Their] findings highlight the potentially important role of these sites in sustaining native species diversity and indicate how socio-cultural factors can present both opportunities and barriers for tropical urban conservation and restoration...”
#sacredsites #antananarivo #madagascar #vegetation #nativeplant #urban #greenspace #tropical #hillsettlements #spatialanalysis #survey #ecosystem #biodiversity #ecology #regeneration #humanimpacts #GIS #spatial #mapping #afrotropics #forest #forestfragments #precolonial #sustainability #diversity #native #sociocultural #restoration -
Match-day Walton pub set for big change as announcement made on its future
https://www.liverpoolecho.co.uk/news/liverpool-news/match-day-walton-pub-set-34255969
The Liverpool ECHO can confirm The Spellow pub has now been sold and its new owners have big plans for the site #EFC #Toffees #Liverpoolsoldproperties #propertynews #GoodisonPark #Regeneration #EvertonFC #Liverpool #Property #Walton -
Beyond Economics: A New Lens on Climate Resilience
Every so often a new report comes out that reminds us all the climate crisis is a health crisis.
#ClimateChange #Environment #OurWonderfulGreenFuture #OWGF #Regeneration #StrongTowns #Urbanism
https://owgf.org/2026/06/10/beyond-economics-a-new-lens-on-climate-resilience/ -
Palette Sorting
Post compost, nerd.https://vianegativa6.wordpress.com/2026/05/30/palette-sorting/
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‘The environmental movement needs many hands’: saving Australia’s biodiversity is getting personal
Powered by individual donations and bequests, the country now has one of the world’s largest networks of privately protected and managed land
by James Norman, Fri 17 Apr 2026
Excerpt: "Over three decades later, #BushHeritage today owns and protects 45 bought or gifted bush reserves around #Australia – covering more than 1.4m hectares of land and providing habitat for over 9,000 native species. Working with #FirstNations communities and farmers, it supports the management of an additional 20.5m hectares beyond what it owns directly.
" 'Some of Australia’s most threatened and fragile #ecosystems and #wildlife sit outside of the national reserve system,' says Rachel Lowry, Bush Heritage Australia’s chief executive officer.
" 'They’re found on private land or pastoral country, and they may have deep cultural and ecological values, but they’re not protected from threats such as #LandClearing, #mining or #InvasiveSpecies.'
"The organisation reports a rise in the number of personal bequests, from 2,500 in 2022 to more than 4,600 bequests last year. Lowry puts the increase down to a personal desire to have a meaningful impact on the worsening global climate and #biodiversity crisis.
"Bush Heritage’s model has inspired other groups around Australia to acquire and conserve lands and forests of significant ecological value. One such example is the #NorthEastTasmania #LandTrust (#NETLT), which secretary Dr Christine Hosking describes as 'a microcosm of Bush Heritage'.
"Formed in 2009, the organisation manages donated land through weed control and regeneration of the native environment.
" 'Currently, we have 10 acres [4 hectares] of endangered eucalyptus forest in #BinalongBay that adjoins the #BayOfFires #Conservation Area, and a smaller patch of coastal habitat for the endangered swift parrot,' she says."
#SolarPunkSunday #LandConservation #EndangeredSpecies #ClimateCrisis #ProtectTheForest #ProtectTheForests #Regeneration #PreserveNature #Conservationists #IndigenousAustralians #TraditionalLandManagement
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#event
#Book presentation: A EMERGÊNCIA DAS SOCIEDADES DE COMUNS (THE EMERGENCE OF COMMONS SOCIETIES)
by Marco de Abreu
📅March 14
🕑4pm to 6pm
📍Cave Cultural @ Rizoma, R. José Estêvão 4, #Lisbon
👥Open to the publicA work describing the author's journey towards Societies of Commons, introducing maps for exploring Regenerative Societies and Societies of Commons, and proposes peaceful, transformative social innovation for Portugal.
#MarcodeAbreu #EcoSystem #Collaboration #Regeneration #CommonGood
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Overconsumption called.
It wants its entire living room back.Time to redecorate the future.
Too-Too, Much-Much by Thomas Hirschhorn, 2010 (installation view, dimensions variable, found objects)
#thomashirschhorn #ClimateArt #Degrowth #PostCapitalism #QueerEcology #MutualAid #Regeneration
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432Hz + 963Hz + 528Hz Relaxing Slavic Blend - Gusli, Domra, Synth Pads and Electronic Beats🎶
https://www.youtube.com/watch?v=EyJqNAqFHPE
#healingmusic #relaxation #sleepmusic #meditation #ambient #folkloric #slavicculture #instrumental #focus #anxietyrelief #stressrelief #deepsleep #regeneration #432hz #963hz #528hz #peaceful #calming #музыкадлясна #расслабление #медитация #фольклор #инструментальнаямузыка #целительнаямузыка #спокойствие
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432Hz + 963Hz + 528Hz Relaxing Slavic Blend - Gusli, Domra, Synth Pads and Electronic Beats🎶
https://www.youtube.com/watch?v=EyJqNAqFHPE
#healingmusic #relaxation #sleepmusic #meditation #ambient #folkloric #slavicculture #instrumental #focus #anxietyrelief #stressrelief #deepsleep #regeneration #432hz #963hz #528hz #peaceful #calming #музыкадлясна #расслабление #медитация #фольклор #инструментальнаямузыка #целительнаямузыка #спокойствие
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432Hz + 963Hz + 528Hz | Feeling Anxious? Try This Healing Folk Sound 🙏
https://www.youtube.com/watch?v=zddrs5wnV_8
#healingmusic #relaxation #sleepmusic #meditation #ambient #folkloric #slavicculture #instrumental #focus #anxietyrelief #stressrelief #deepsleep #regeneration #432hz #963hz #528hz #peaceful #calming #музыкадлясна #расслабление #медитация #фольклор #инструментальнаямузыка #целительнаямузыка #спокойствие
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Kommt der #Kipppunkt, an dem #Chaos in #Systemversagen / #Systemzusammenbruch mündet, gibt es einen #Wandel des #Systems z.B. hin zu #repressiver #Unterdrückung unter #Bedingungen #elendem #Prekariats, oder #regeneriert sich das #System durch #innovative #Anpassung an seine sich #verändernde #Umwelt? (vermutlich alles drei #gleichzeitig)
https://ifwo.eu/@sozialwelten/114782207335088635
#prekär #Repression #Regeneration #Adaptabilität #Krise #Klimakrise
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My next @preLights post is out. I saw this first preprint from the Storer lab a while back (thanks to a toot from @CellySally) and took some time over writing it but I think it was worth it!
I really enjoyed going back to some developmental biology with this study on how the ECM and mechanical environment influence limb regeneration. Also, nice that my PhD department, PDN, was heavily involved!
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#ArtAdventCalendar Day 21 #WinterSolstice #MidWinter here #MidSummer for those on the currently sunny side of the globe!
In the North we celebrate the beginning of the slow return of #Saule #Sun but don't forget to give #Darkness its due: life, #regeneration begins in the darkness of blood, soil, womb, heart, brain! Darkness is #rest #nest Take time to repose and #reflect and appreciate the long quiet nights.
#AncientWays #pagan #animist #GoddessCulture #Latvian #Ragana #Alberta #WinterBright -
A preview of the improvised masks I made for #WinterSolstice Tomorrow is a work day, so I plan to drag the Jul log today- these masks are for that- though prob just for a photo op as they aren't super functional..lol- just paper + measurements weren't what they might have been, but lessons learned! #Ragana #Baltic #Goddess of #nature #balance #death and #regeneration is strongest at the time of Winter Solstice; we celebrate her #darkness + #Saule #Sun we #celebrate the beginning of her return
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... stemcell research @Vitruvian Project
#stemcell #regeneration #vitruvianproject #organ #labgrown #dna #sciencehorror #sciencefiction #horror #horrorart #aiia #macabre #horrormovies #darkart #nightmare
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... stem cell/regeneration test #0167 ...
#stemcell #regeneration #organ #labgrown #dna #sciencehorror #sciencefiction #horror #horrorart #aiia #macabre #horrormovies #darkart #nightmare
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If you're familiar with #EasternHemlock #forests then you might picture them as a closed #canopy with sparse vegetation in the #understory.
But when a fallen tree opens up that canopy, the #gap supports a profusion of #regeneration.
Very cool to find this understory #heterogeneity at #RBWinterStatePark in #Pennsylvania.
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Wow! Not often you find such immersive stories, art and design in one place! Warm recommendation! ☺️
https://www.youtube.com/watch?v=B5Yro8J8r8Q
Check out their website too: https://stories.life/
#StoriesForLife #WellbeingEconomy #SystemChange #Regeneration #ClimateJustice #DoughnutEconomics #Sustainability #ClimateCrisis #Wellbeing #Cooperatives #Storytelling #Solarpunk #ImaginationActivism
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Beavers are Scotland's secret weapon in #rewilding fight
‘Could 2023 be the year that we see #beavers being reintroduced widely across #Scotland? For that to happen, Scotland’s Government bodies, which between them manage 10% of the country’s land, need to step up.’
#biodiversity #ecosystems #nature #restoration #regeneration #interconnected #interdependent #deought #wetlands #climate #resilience
https://www.thenational.scot/comment/23267642.beavers-scotlands-secret-weapon-rewilding-fight/
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CW: Using Human Pluripotent Stem Cells to Create Human Skeletal Muscle Organoids for Repair and Regeneration
Skeletal #muscle is a type of tissue that makes up a large part of the human body. It is made up of many different cells that are able to contract and move. Skeletal muscle has the ability to #repair itself when it is damaged due to #aging, exercise, or diseases like #MuscularDystrophy. A small group of cells called #SatelliteCell s help with the repair process. Scientists have been trying to create models to study how #Skeletalmuscle develops and regenerates. Recently, they have been using human pluripotent #StemCell to create 3D models of skeletal muscle tissue. However, these models have not been able to recreate the full process of muscle regeneration. In this research paper, the authors introduce a new method of using human pluripotent stem cells to create 3D models of skeletal muscle tissue that can retain the ability to repair itself.
Over the past decades, scientists have used #animalmodel to study #muscleregeneration, which is regulated by #stemcell s. These animal models have been very helpful in understanding the mechanisms of muscle #regeneration, but they don't always accurately reflect the same range of diseases that humans experience. Therefore, researchers have suggested creating reliable in vitro models using human muscle cells. ( #hPSC s) could be used to create 3D human skeletal muscle #organoid s ( #hSkMO s) that contain sustainable #stemcell and distinct myofibers with the same proteins and structure as adult muscles. Previous approaches to skeletal muscle differentiation have been developed using 2D #culture systems, but these lack the natural environment and #StemCell niche that are necessary to model adult #myogenesis and muscle #regeneration.
#Stemcell s ( #SC s) can be used to repair damaged muscle tissue. They explain that SCs can be activated in response to muscle injuries and that other #cell types can contribute to the process of #myogenesis. The author then goes on to explain that #cytokine s, such as IL-4, can influence the #InflammatorySystem and promote SCs differentiation, which helps with muscle regeneration. While #organoid s generated from #hPSC s have potential, they do not fully replicate the in vivo native microenvironment. To address this, treat the #hSkMO s with extrinsic #cytokine s to promote #muscle #regeneration . #hSkMO s might then be used to study aspects of human muscle #biology and to identify novel #therapeutic candidates for muscle-wasting disorders.
To create a 3D structure of muscle tissue. They used #WNT activator and #BMP inhibitors at the beginning of the differentiation process to induce paraxial #mesodermal #cell s. They then added #FGF2 to the Matrigel to promote the 3D structure. #HGF and IGF1 were added later to accelerate the #myogenic specification and further #myofiber differentiation. They optimized the timing of the Matrigel embedding to day seven. After this, they observed #neuralcell s and withdrew FGF2 to focus on muscle tissue development. They then prolonged the HGF and IGF1 treatment to propagate #myogenic #progenitor s. They found that 62% of the #tissue was #skeletalmuscle tissue and that it contained PAX7+ #myogenic #stem / #progenitor cells, MYOD+ activated/committed #myoblast s, and MYOG+ #myocyte s. They also found that 31% of PAX7+/Ki67− and 29% of MYOD−/PAX7+ non-dividing quiescent SCs were present in the mature #hSkMO s. This indicates that the #hSkMO s were able to effectively recreate #embryo nic #myogenesis and have regenerative potential. Future studies using #singlecell #RNA sequencing may be necessary to further characterize the different types of cells in #hSkMO s.
The stepwise process to generate human skeletal muscle organoid s (hSkMOs) from human pluripotent stem cells (hPSCs)
The process begins with dissociating #hPSC s into #singlecell s and allowing them to form #embryoid bodies ( #EB s) in low-attachment V-shaped 96-well plates. Then, paraxial #mesodermal differentiation is promoted with #WNT activation, BMP inhibition, and FGF2 signaling. The expression of pluripotency markers OCT4 and NANOG decreases, and the expression of #mesoderm markers Brachyury, T-Box transcription factor 6 (TBX6), and mesogenin 1 (MSGN1) increases. To further characterize paraxial #mesoderm al differentiation, TBX6 is #immunostain ed. After paraxial #mesodermal induction, the #organoid s are embedded with growth factor-reduced Matrigel and transferred to a six-well plate on an orbital shaker. Growth factors are then added to the #myogenic specification media, and #hSkMO s are cultured until the day of analysis. The orbital shaker improves the viability, survival, and differentiation of hSkMOs by increasing the penetration rate of oxygen and nutrients into the core area of hSkMOs. The #hSkMOs gradually grow to more than 1.5 mm in diameter by day 60, appearing round-shaped, uniformly sized, and having relatively homogenous morphology. PAX3 and PAX7 are #myogenic progenitor markers, and their expression is verified by qRT-PCR and #cryo sections. The #myogenic cells appear as clusters, and approximately 9% of PAX7+ cells are double-positive for Ki67 at day 30, demonstrating that proliferating cells are #myogenic #progenitor s in hSkMOs. This indicates that the in vitro #hSkMO #culturesystem is able to recapitulate the features of embryonic skeletal #muscle development.
The different types of #SkeletalMuscle stem/progenitor cells that are involved in myogenesis, the process of muscle formation.
The researchers used qRT-PCR analysis and #immunohistochemistry to identify and characterize the different types of cells. They found that PAX3 and PAX7 (SC markers) were the major population during the early stage of #myogenesis, and that MYOD (proliferating and activated SC marker) and MYOG (differentiated myocyte marker) increased over time. They also observed that MYOD−/PAX7+, MYOD+/PAX7+, and MYOD+/Ki67+ cells accounted for 29%, 6%, and 8% of the putative quiescent, activated, and proliferating #SC s, respectively. MYOD+/PAX7− cells constituted 39% of differentiating myoblasts, and MYOG−/PAX7+ cells constituted 23% of putative quiescent SCs. MYOG+/PAX7− cells accounted for 30% of differentiated #myocyte s, and 8% and 6% of the MYOG+ cells in #hSkMO s co-expressed PAX7 and Ki67, respectively. This data shows that the researchers were able to identify and characterize different types of skeletal muscle stem/progenitor cells during #myogenesis.
The text is discussing the results of a research study that used hSkMOs (human skeletal muscle #organoid s) to study the development of skeletal muscle #tissue. The study found that the #hSkMO s grew exponentially in size within two months, and the growth rate then steadily decreased. The researchers then used scanning electron microscopy (SEM) imaging and confocal microscopy to examine the cytoarchitecture of the hSkMOs. They found that the hSkMOs contained a large population of terminally differentiated #myogenic cells and a small population of preserved myogenic stem/progenitor cells. They also found that the hSkMOs contained a substantial proportion of TITIN+ muscle cells and MAP2-positive #neuron s. To further characterize the presence of sustainable stem cells within the mature hSkMOs, they quantified the amount of dormant stem cells by #confocal #microscopy imaging. The results showed that approximately 56%, 31%, and 5% of PAX7+/Ki67- putative dormant stem cells existed throughout the differentiation of hSkMOs at days 30, 70, and 130, respectively. This indicates that the hSkMOs contained mature skeletal muscle properties and had the potential for #regeneration .
The researchers wanted to see if the #hSkMO s (human #skeletal muscle #organoid s) had the ability to regenerate #muscle #tissue after damage. To test this, they treated the hSkMOs with a cardiotoxin (CTX) which is known to induce muscle inflammation and damage. They then observed a decrease in PAX7+ and MYOD+ cells in the hSkMOs. To further test the #regenerative potential of the #hSkMO s, they added interleukin-4 (IL-4) to the medium to promote #muscleregeneration. After 14 days, they observed a significant increase in MYOG+ myocytes in the CTX-injured hSkMOs with the treatment of IL-4 compared to the CTX-injured hSkMOs without the treatment. This suggests that the hSkMOs have the potential to regenerate muscle tissue after damage.
Generation of Skeletal Muscle Organoids from Human Pluripotent Stem Cells to Model Myogenesis and Muscle Regeneration
Authors :
Min-Kyoung Shin , Jin Seok Bang , Jeoung Eun Lee , Hoang-Dai Tran , Genehong Park , Dong Ryul Lee and Junghyun Jo
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Refurbished flats at the #ParkHill Estate, #Sheffield. Bricks and concrete. #brutalist #photography #architecture #gentrification #regeneration #housing and maybe a nod to the colour palette of #LeCorbusier #MachineForLiving
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amputated legs after treatment with a chemical cocktail | New Scientist
https://www.newscientist.com/article/2306066-frogs-regrow-amputated-legs-after-treatment-with-a-chemical-cocktail/#BioDome #silk_gel #Xenopus_laevis #frog #hindlimb #amputation #regrowth #regeneration