#barcoding — Public Fediverse posts

RT by @Plants_EFSA: Behind the scenes, indentify #2 : @aimethods DNA Barcoding.

Black-headed leafcutter bee, Megachile circumcincta. The DNA was isolated from a leg of the bee using a liquid (lysis buffer). Means everything dissolves in it except for the chitin. Then the Co1 gene was amplified with PCR. This means that certain parts of the DNA are copied so often that they are easily detectable. Detectable for the sequencer, the next step. The 313 base pairs required for the identification are then determined here. (See colorful graphic) With the help of a worldwide database of already certain animals whose Co1 gene has been determined, the base pairs can be compared with each other.

#dnabarcoding #barcoding #dna #macro #insects #bumblebee #hummel #insekten

🐦🔗: https://nitter.cz/sagaOptics/status/1725182377717108888#m

[2023-11-16 16:01 UTC]

One of the best uses of #DNA 🧬 #barcoding is linking different life stages of single #species that appear different from each other and do different things.

In this paper the authors used barcoding to figure out which #polyp (sedentary) and #medusa (swimming) life stages of several #hydrozoans (related to #sea #jellies and sea #anemones) belong to each other.

🔗: https://peerj.com/articles/15118/

Title: Odd family reunion: DNA barcoding reveals unexpected relationship between three hydrozoan species

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#introduction
Hi everyone! 👋 First time I'm tooting!
I'm an Italian #entomologist doing my #PhD at the Institute of Evolutionary Biology #IBE in Barcelona.

I study the #diversity and #evolution of the European #ant fauna using #IntegrativeTaxonomy, #genetics (mainly #barcoding), and #genomics (#UCEs). I also work on #InvasiveAlienSpecies and #butterfly #phylogeography.

I love beer, #Rstats, maps, and turning over stones. Ah, I'm also an #iNaturalist addict!