#metabarcoding — Public Fediverse posts

Pipeline release! nf-core/ampliseq v2.17.0 - Ampliseq Version 2.17.0!
Amplicon sequencing analysis workflow using DADA2 and QIIME2
Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.17.0

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #metataxonomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/ampliseq v2.17.0 - Ampliseq Version 2.17.0!
Amplicon sequencing analysis workflow using DADA2 and QIIME2
Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.17.0

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #metataxonomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/ampliseq v2.17.0 - Ampliseq Version 2.17.0!
Amplicon sequencing analysis workflow using DADA2 and QIIME2
Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.17.0

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #metataxonomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/ampliseq v2.17.0 - Ampliseq Version 2.17.0!
Amplicon sequencing analysis workflow using DADA2 and QIIME2
Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.17.0

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #metataxonomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/ampliseq v2.17.0 - Ampliseq Version 2.17.0!
Amplicon sequencing analysis workflow using DADA2 and QIIME2
Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.17.0

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #metataxonomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/ampliseq v2.16.1 - Ampliseq release 2.16.1 2026-01-23!
Amplicon sequencing analysis workflow using DADA2 and QIIME2
Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.16.1

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #metataxonomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/ampliseq v2.16.1 - Ampliseq release 2.16.1 2026-01-23!
Amplicon sequencing analysis workflow using DADA2 and QIIME2
Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.16.1

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #metataxonomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/ampliseq v2.16.1 - Ampliseq release 2.16.1 2026-01-23!
Amplicon sequencing analysis workflow using DADA2 and QIIME2
Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.16.1

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #metataxonomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/ampliseq v2.16.1 - Ampliseq release 2.16.1 2026-01-23!
Amplicon sequencing analysis workflow using DADA2 and QIIME2
Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.16.1

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #metataxonomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/ampliseq v2.16.1 - Ampliseq release 2.16.1 2026-01-23!
Amplicon sequencing analysis workflow using DADA2 and QIIME2
Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.16.1

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #metataxonomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/ampliseq v2.16.0 - Ampliseq Version 2.16.0!
Amplicon sequencing analysis workflow using DADA2 and QIIME2
Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.16.0

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #metataxonomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/ampliseq v2.16.0 - Ampliseq Version 2.16.0!
Amplicon sequencing analysis workflow using DADA2 and QIIME2
Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.16.0

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #metataxonomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/ampliseq v2.16.0 - Ampliseq Version 2.16.0!
Amplicon sequencing analysis workflow using DADA2 and QIIME2
Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.16.0

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #metataxonomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/ampliseq v2.16.0 - Ampliseq Version 2.16.0!
Amplicon sequencing analysis workflow using DADA2 and QIIME2
Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.16.0

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #metataxonomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/ampliseq v2.16.0 - Ampliseq Version 2.16.0!
Amplicon sequencing analysis workflow using DADA2 and QIIME2
Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.16.0

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #metataxonomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Our new @[email protected] paper on Advancing ecological #assessment: towards the integration of #eDNA #metabarcoding into an estuarine #fish index is out. #Research #MarineEcology #GES4SEAS Free download here: authors.elsevier.com/sd/article/S...

Pipeline release! nf-core/ampliseq v2.15.0 - Ampliseq Version 2.15.0!

Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.15.0

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/detaxizer v1.2.0 - detaxizer 1.2.0 - Kunschd!

Please see the changelog: https://github.com/nf-core/detaxizer/releases/tag/1.2.0

#deidentification #decontamination #edna #fastq #filter #longreads #metabarcoding #metagenomics #microbiome #nanopore #shortreads #shotgun #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/ampliseq v2.14.0 - Ampliseq Version 2.14.0!

Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.14.0

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/ampliseq v2.13.0 - Ampliseq Version 2.13.0!

Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.13.0

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Took a while, but my largest ever upload of data to the #ENA / #SRA is live, nearly 4000 #metabarcoding amplicon sequencing runs using a #Phytophthora ITS1 marker: https://www.ebi.ac.uk/ena/browser/view/PRJEB76241

Managing sequencing #metadata is hard!

Pipeline release! nf-core/ampliseq v2.12.0 - Ampliseq Version 2.12.0!

Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.12.0

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/detaxizer v1.1.0 - detaxizer 1.1.0 - Kombjuudr!

Please see the changelog: https://github.com/nf-core/detaxizer/releases/tag/1.1.0

#deidentification #decontamination #edna #fastq #filter #longreads #metabarcoding #metagenomics #microbiome #nanopore #shortreads #shotgun #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

🦠 Interested in #nextflow & anything 'meta-' ?(#metagenomics, #metabarcoding, #metatranscriptomics, #metaproteomics, #microbes #MicrobialEcology & more!)

📆 Join us next Tuesday 13:00 CEST (3rd September) for an nf-core #bytesize for the #metaomics #nfcore special interest group! (Also on YouTube after!)

🤝 We will introduce how we want the community to work together with #users and #bioinformatics developers to make the best pipelines for anything 'meta-'

ℹ️ Zoom: https://nf-co.re/events/2024/bytesize_sig_meta_omics

Pipeline release! nf-core/ampliseq v2.11.0 - Ampliseq Version 2.11.0!

Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.11.0

#16s #18s #amplicon-sequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #microbiome #pacbio #qiime2 #rrna #taxonomic-classification #taxonomic-profiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/ampliseq v2.10.0 - Ampliseq Version 2.10.0!

Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.10.0

#16s #18s #amplicon-sequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #microbiome #pacbio #qiime2 #rrna #taxonomic-classification #taxonomic-profiling #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/ampliseq v2.9.0 - Ampliseq Version 2.9.0!

Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.9.0

#16s #18s #amplicon-sequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #microbiome #pacbio #qiime2 #rrna #taxonomic-classification #taxonomic-profiling #nfcore #openscience #nextflow #bioinformatics

If this all-singing-all-dancing new PCR machine works as well is it sounds http://omicsomics.blogspot.com/2024/02/iconpcr-super-flexible-qpcr.html (blog post from @OmicsOmics) it would be amazing for a bunch of stuff including #metabarcoding #amplicon sequencings where #eDNA and other samples are often at different concentrations.

Happy to have contributed, w Thorsten Stoeck,
@ionferrico and others, to this paper by Leontidou et al where we describe our results from trying out a new type of commercial assay for #metabarcoding #microbial communities of several #16S hypervariable regions at once. Do check it out.

#MicrobialEcology #Molecular #Methods

https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.16530

Just published: "Marine invasive alien species in Europe: 9 years after the #IAS Regulation"

Proud to contribute to the #eDNA part of this review on #BioMonitoring of #marine #invasive #alien species in Europe, as part of the #GES4SEAS project. Have a look, esp. if you wondered how many #NIS species for which sequence vouchers exist for #DNA #barcoding or #metabarcoding

https://www.frontiersin.org/articles/10.3389/fmars.2023.1271755/full?utm_source=F-NTF&utm_medium=EMLX&utm_campaign=PRD_FEOPS_20170000_ARTICLE

🚀 Registration for the 7th edition of the #Metabarcoding in #MicrobialEcology course is now open!

📚 Dive into the world of #Metabarcoding and #Bioinformatics with #qiime2 and #Rstats

🔗 Register here: https://www.physalia-courses.org/courses-workshops/course30/

Subsampling #sediment cores from Basque #seagrass (#Zostera noltii) meadows and saltmarshes for #environmental #DNA and #ancient #eDNA analyses (for #prokaryotes, #fungi #meiofauna, and maybe #plant #community analysis)

#MicrobialEcology #metabarcoding #environmentalmicrobiology #EnvironmentalGenomics #BlueCarbon #wetlands #SaltMarsh #seagrassmeadows #sedaDNA

#Freshwater #mussels, many of which are of #conservation #concern, are a great target for #environmental #DNA (#eDNA) detection to guide #management #tactics and #strategies.

But as the authors of this new paper point out: "Confidence in our ability to identify eDNA #metabarcoding reads relies on a #curated, #accurate #database."

Use of eDNA methods various purposes is going to mean a lot of work with #specimens and databases in coming years.

🧬 #biodiversity

🔗 https://peerj.com/articles/15127/

Announcing the release of version 4.14.1 of the PR2 database of 18S eukaryotic reference sequences.

The database itself is unchanged, but we now provide a web interface:

https://app.pr2-database.org

More information: https://pr2database.github.io/pr2database/articles/vignette-shiny-presentation.html

#protists #metabarcoding #database #18S

Announcing the release of version 2.0 of metaPR2, database of 18S eukaryotic metabarcodes

https://shiny.metapr2.org/metapr2/

Major changes are listed here: https://pr2database.github.io/metapr2-shiny/news/index.html

* Addition of 18 new datasets
* Use of clustered metabarcodes (ASVs)
* New panel listing taxonomy of metabarcodes
* Barplots now include samples where taxa are absent

Please report any issue here: https://matrix.to/#/#pr2-database:matrix.org

#protists #metabarcoding #biogeography #18S

📢Article Alert from Marine Biodiversity Center, NHMLAC
"A manager’s guide to using #eDNA #metabarcoding in marine ecosystems" https://peerj.com/articles/14071 #ConservationBiology #EcosystemScience #MarineBiology #SciencePolicy #EnvironmentalImpacts

Hi all, curious if anyone has run into this kind of issue before? We're quantifying our #amplicon #PCR reactions for #Metabarcoding using PicoGreen prior to normalisation, but we're sometimes getting high concentrations despite no band being visible on a gel. For ITS it’s only at 61 ng/ul that there’s a 50/50 chance of seeing a band. Any idea what might be going on?

#Microbiology #MolecularBiology #labwork