#nanopore — Public Fediverse posts
Live and recent posts from across the Fediverse tagged #nanopore, aggregated by home.social.
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@nanopore My selected novel statements from ONT's Tech Update from the 2024 Nanopore Community meeting:
* Updated documentation centre is public: no need to log in
* For large-scale human sequencing (thousands and thousands of individuals), >90% runs over 100 Gb (30X)
* 24-plex direct RNA barcoding kit
* Improved enzyme motor speed, ONT have got a flow cell over 270 Gb yield
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new citation:
`Using salt tolerance compartmentalized self-replication (stCSR) and a #microfluidic platform, we obtained 11 mutant sites with enhanced salt tolerance attributes. #Sequencing and #biochemical analyses revealed that the substitution of conserved amino acids such as G197D, Y369E, T372N, and I378R plays a critical role in maintaining the #processivity of exonuclease-deficient #phi29 #polymerase under high salt conditions.`
https://www.frontiersin.org/articles/10.3389/fmicb.2023.1267196/full
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Hello #NanoporeConf! Join me at 15:35 EST (20:35 GMT) for an online bioinformatics speakeasy. We'll chat in hushed tones over cocktails (BYOB) about #nanopore bioinformatics. Stop by and ask me about our recent EPI2ME Labs release! Access through the app, password is applepore...
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Exciting #nanopore updates for RNA biologists announced last night. FINALLY some new chemistry development for direct RNAseq & base caller model updates to go with! ONT's developed many bespoke helicases via an ML-guided engineering effort in 2022, and we're getting one for RNA. Supposedly faster & more consistent motor speeds, improved RNA base calling with accuracy up to 96%, and 3X output improvements from the chemistry changes. All coming next year, early access in Q1.
#NCM2022 #NanoporeConf -
Poster + slides done and uploaded. Now to practice the lightning talk until #NCM2022 in December. 4 minutes means I need to talk really fast, right? #nanopore #sequencing