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#nanoporesequencing — Public Fediverse posts

Live and recent posts from across the Fediverse tagged #nanoporesequencing, aggregated by home.social.

  1. Dear Nanopore folks, could you give an advice about an issue that my lab has with direct RNA sequencing on MinION. We're doing RNA004 sequencing of various samples and again and again, there's a staggering amount of reads with just repetitive AAG triplet. This happens for weird IVT RNA and perfectly normal yeast or HEK cells. I'm using Dorado 1.4.0 with the 5.3.0 sup model. Looks to me like the open channel or some kind of stalling is just called as this kmer.
    #nanopore #nanoporesequencing

  2. Dear Nanopore folks, could you give an advice about an issue that my lab has with direct RNA sequencing on MinION. We're doing RNA004 sequencing of various samples and again and again, there's a staggering amount of reads with just repetitive AAG triplet. This happens for weird IVT RNA and perfectly normal yeast or HEK cells. I'm using Dorado 1.4.0 with the 5.3.0 sup model. Looks to me like the open channel or some kind of stalling is just called as this kmer.
    #nanopore #nanoporesequencing

  3. Dear Nanopore folks, could you give an advice about an issue that my lab has with direct RNA sequencing on MinION. We're doing RNA004 sequencing of various samples and again and again, there's a staggering amount of reads with just repetitive AAG triplet. This happens for weird IVT RNA and perfectly normal yeast or HEK cells. I'm using Dorado 1.4.0 with the 5.3.0 sup model. Looks to me like the open channel or some kind of stalling is just called as this kmer.
    #nanopore #nanoporesequencing

  4. Dear Nanopore folks, could you give an advice about an issue that my lab has with direct RNA sequencing on MinION. We're doing RNA004 sequencing of various samples and again and again, there's a staggering amount of reads with just repetitive AAG triplet. This happens for weird IVT RNA and perfectly normal yeast or HEK cells. I'm using Dorado 1.4.0 with the 5.3.0 sup model. Looks to me like the open channel or some kind of stalling is just called as this kmer.
    #nanopore #nanoporesequencing

  5. Does anyone know if an NVidia RTX 4060 can handle an Mk1D MinION with runtime basecalling? ONT's documentation says that the minimum requirement is an RTX 5060, but this sounds a bit crazy. And the difference between the two GPUs is not that big I think.

    Also, this will mostly be for dRNA-seq, where we basecall after the run, so would it be okay to run two MinIONs in parallel (without basecalling)?

    #nanopore #nanoporeSequencing #OxfordNanopore