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1000 results for “quantixed”
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si vous aimez les protéines dans vos repas ou que vous en avez besoin, mais vous n'avez pas trop les moyens pour de la viande, pensez au chorizo premier prix.
c'est salé, goûtu, gras, pimenté. Mis à rissoler en cubes avec des oignons et des dés de carottes avant d'y jeter du riz, et la quantité d'eau que vous auriez mis pr le riz (un peu moins de sel), ça vous fait un très bon plat roboratif et pas cher au #ricecooker.
#recette #bonplan #cheap #bon #cuisine #miam #yum #gourmand #pascher
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Infomigrants
"Soit tu passes, soit tu meurs" : au Tchad, des Soudanais racontent leur fuite d’El-Fasher
" tu dois passer par la tranchée. Ils l’ont creusée tout autour d’El-Fasher. Il y a une quantité de cadavres dans cette tranchée."
https://mcinformactions.net/soit-tu-passes-soit-tu-meurs-au-tchad-des-soudanais-racontent-leur-fuite-d-el
#Soudan #Tchad #refugies #exiles #migrants #immigration -
ERDAL ARIKAN WAS born in 1958 and grew up in Western Turkey, the son of a doctor and a homemaker.
He loved science.
When he was a teenager, his father remarked that, in his profession, two plus two did not always equal four.
This fuzziness disturbed young Erdal; he decided against a career in medicine. He found comfort in engineering and the certainty of its mathematical outcomes.
“I like things that have some precision,” he says. “You do calculations and things turn out as you calculate it.”
Arıkan entered the electrical engineering program at Middle East Technical University. But in 1977, partway through his first year, the country was gripped by political violence, and students boycotted the university.
Arıkan wanted to study, and because of his excellent test scores he managed to transfer to #CalTech, one of the world's top science-oriented institutions, in Pasadena, California.
He found the US to be a strange and wonderful country. Within his first few days, he was in an orientation session addressed by legendary physicist #Richard #Feynman. It was like being blessed by a saint.
Arıkan devoured his courses, especially in #information #theory.
The field was still young, launched in 1948 by #Claude #Shannon, who wrote its seminal paper while he was at Bell Labs;
he would later become a revered MIT professor.Shannon's achievement was to understand how the hitherto fuzzy concept of information could be quantified, creating a discipline that expanded the view of communication and data storage.
By publishing a general mathematical theory of information
—almost as if Einstein had invented physics and come up with relativity in one swoop
—Shannon set a foundation for the internet, mobile communications, and everything else in the digital age.The subject fascinated Arıkan, who chose #MIT for graduate studies.
There was one reason: “#Bob #Gallager was there,” he says.
Robert Gallager had written the textbook on information theory. He had also been mentored by Shannon's successor.
In the metrics of the field, that put him two steps from God.
“So I said, if I am going to do information theory,” Arıkan says, “MIT is the place to go.”
By the time Arıkan arrived at MIT, in 1981, Gallager had shifted his focus and was concentrating on how data networks operated.
Arıkan was trembling when he went to Gallager's office for the first time. The professor gave him a paper about packet radio networks.
“I was pushing him to move from strict information theory to looking at network problems,” Gallager says.
“It was becoming very obvious to everyone that sending data from one place to another was not the whole story
—you really had to have a system.”#Guo #Ping #Huawei #Ren #Zhengfei #gold #medal #honored #guest #Erdal #Arıkan #5G #technology #daughter
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ERDAL ARIKAN WAS born in 1958 and grew up in Western Turkey, the son of a doctor and a homemaker.
He loved science.
When he was a teenager, his father remarked that, in his profession, two plus two did not always equal four.
This fuzziness disturbed young Erdal; he decided against a career in medicine. He found comfort in engineering and the certainty of its mathematical outcomes.
“I like things that have some precision,” he says. “You do calculations and things turn out as you calculate it.”
Arıkan entered the electrical engineering program at Middle East Technical University. But in 1977, partway through his first year, the country was gripped by political violence, and students boycotted the university.
Arıkan wanted to study, and because of his excellent test scores he managed to transfer to #CalTech, one of the world's top science-oriented institutions, in Pasadena, California.
He found the US to be a strange and wonderful country. Within his first few days, he was in an orientation session addressed by legendary physicist #Richard #Feynman. It was like being blessed by a saint.
Arıkan devoured his courses, especially in #information #theory.
The field was still young, launched in 1948 by #Claude #Shannon, who wrote its seminal paper while he was at Bell Labs;
he would later become a revered MIT professor.Shannon's achievement was to understand how the hitherto fuzzy concept of information could be quantified, creating a discipline that expanded the view of communication and data storage.
By publishing a general mathematical theory of information
—almost as if Einstein had invented physics and come up with relativity in one swoop
—Shannon set a foundation for the internet, mobile communications, and everything else in the digital age.The subject fascinated Arıkan, who chose #MIT for graduate studies.
There was one reason: “#Bob #Gallager was there,” he says.
Robert Gallager had written the textbook on information theory. He had also been mentored by Shannon's successor.
In the metrics of the field, that put him two steps from God.
“So I said, if I am going to do information theory,” Arıkan says, “MIT is the place to go.”
By the time Arıkan arrived at MIT, in 1981, Gallager had shifted his focus and was concentrating on how data networks operated.
Arıkan was trembling when he went to Gallager's office for the first time. The professor gave him a paper about packet radio networks.
“I was pushing him to move from strict information theory to looking at network problems,” Gallager says.
“It was becoming very obvious to everyone that sending data from one place to another was not the whole story
—you really had to have a system.”#Guo #Ping #Huawei #Ren #Zhengfei #gold #medal #honored #guest #Erdal #Arıkan #5G #technology #daughter
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ERDAL ARIKAN WAS born in 1958 and grew up in Western Turkey, the son of a doctor and a homemaker.
He loved science.
When he was a teenager, his father remarked that, in his profession, two plus two did not always equal four.
This fuzziness disturbed young Erdal; he decided against a career in medicine. He found comfort in engineering and the certainty of its mathematical outcomes.
“I like things that have some precision,” he says. “You do calculations and things turn out as you calculate it.”
Arıkan entered the electrical engineering program at Middle East Technical University. But in 1977, partway through his first year, the country was gripped by political violence, and students boycotted the university.
Arıkan wanted to study, and because of his excellent test scores he managed to transfer to #CalTech, one of the world's top science-oriented institutions, in Pasadena, California.
He found the US to be a strange and wonderful country. Within his first few days, he was in an orientation session addressed by legendary physicist #Richard #Feynman. It was like being blessed by a saint.
Arıkan devoured his courses, especially in #information #theory.
The field was still young, launched in 1948 by #Claude #Shannon, who wrote its seminal paper while he was at Bell Labs;
he would later become a revered MIT professor.Shannon's achievement was to understand how the hitherto fuzzy concept of information could be quantified, creating a discipline that expanded the view of communication and data storage.
By publishing a general mathematical theory of information
—almost as if Einstein had invented physics and come up with relativity in one swoop
—Shannon set a foundation for the internet, mobile communications, and everything else in the digital age.The subject fascinated Arıkan, who chose #MIT for graduate studies.
There was one reason: “#Bob #Gallager was there,” he says.
Robert Gallager had written the textbook on information theory. He had also been mentored by Shannon's successor.
In the metrics of the field, that put him two steps from God.
“So I said, if I am going to do information theory,” Arıkan says, “MIT is the place to go.”
By the time Arıkan arrived at MIT, in 1981, Gallager had shifted his focus and was concentrating on how data networks operated.
Arıkan was trembling when he went to Gallager's office for the first time. The professor gave him a paper about packet radio networks.
“I was pushing him to move from strict information theory to looking at network problems,” Gallager says.
“It was becoming very obvious to everyone that sending data from one place to another was not the whole story
—you really had to have a system.”#Guo #Ping #Huawei #Ren #Zhengfei #gold #medal #honored #guest #Erdal #Arıkan #5G #technology #daughter
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ERDAL ARIKAN WAS born in 1958 and grew up in Western Turkey, the son of a doctor and a homemaker.
He loved science.
When he was a teenager, his father remarked that, in his profession, two plus two did not always equal four.
This fuzziness disturbed young Erdal; he decided against a career in medicine. He found comfort in engineering and the certainty of its mathematical outcomes.
“I like things that have some precision,” he says. “You do calculations and things turn out as you calculate it.”
Arıkan entered the electrical engineering program at Middle East Technical University. But in 1977, partway through his first year, the country was gripped by political violence, and students boycotted the university.
Arıkan wanted to study, and because of his excellent test scores he managed to transfer to #CalTech, one of the world's top science-oriented institutions, in Pasadena, California.
He found the US to be a strange and wonderful country. Within his first few days, he was in an orientation session addressed by legendary physicist #Richard #Feynman. It was like being blessed by a saint.
Arıkan devoured his courses, especially in #information #theory.
The field was still young, launched in 1948 by #Claude #Shannon, who wrote its seminal paper while he was at Bell Labs;
he would later become a revered MIT professor.Shannon's achievement was to understand how the hitherto fuzzy concept of information could be quantified, creating a discipline that expanded the view of communication and data storage.
By publishing a general mathematical theory of information
—almost as if Einstein had invented physics and come up with relativity in one swoop
—Shannon set a foundation for the internet, mobile communications, and everything else in the digital age.The subject fascinated Arıkan, who chose #MIT for graduate studies.
There was one reason: “#Bob #Gallager was there,” he says.
Robert Gallager had written the textbook on information theory. He had also been mentored by Shannon's successor.
In the metrics of the field, that put him two steps from God.
“So I said, if I am going to do information theory,” Arıkan says, “MIT is the place to go.”
By the time Arıkan arrived at MIT, in 1981, Gallager had shifted his focus and was concentrating on how data networks operated.
Arıkan was trembling when he went to Gallager's office for the first time. The professor gave him a paper about packet radio networks.
“I was pushing him to move from strict information theory to looking at network problems,” Gallager says.
“It was becoming very obvious to everyone that sending data from one place to another was not the whole story
—you really had to have a system.”#Guo #Ping #Huawei #Ren #Zhengfei #gold #medal #honored #guest #Erdal #Arıkan #5G #technology #daughter
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Estimate the mKBO (multi-group Kitagawa-Blinder-Oaxaca) decomposition Use mkbo (mKBO) With (In) R Software
https://s.id/_mkbo
Quantile approach by Combes et al. (2012) Use estquant With STATA 19
https://s.id/estquant
#rstudio #rsoftware #softwarer #stata #stata19 #olahdatasemarang #programming -
After quite some weeks of work I have quantified the #numerical error of several #FFT implementations compared to my own.
This was done by implementing an arbitrary precision FFT to compute the true value.
For each implementation we compare the L1, L2 and Linfinity norms as well as #rms
The theoretical l2 bound is derived by Nicholas J.Higham in his famous book 'accuracy and stability of numerical algorithms'
The Linf bound from P. Henrici 'Applied and Computational Complex Analysis'
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After quite some weeks of work I have quantified the #numerical error of several #FFT implementations compared to my own.
This was done by implementing an arbitrary precision FFT to compute the true value.
For each implementation we compare the L1, L2 and Linfinity norms as well as #rms
The theoretical l2 bound is derived by Nicholas J.Higham in his famous book 'accuracy and stability of numerical algorithms'
The Linf bound from P. Henrici 'Applied and Computational Complex Analysis'
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Tout le monde pète un plomb avec le prêt à porter. Les tailles ne conviennent à personne, si c’est serré sur les fesses ça flotte à la taille, ça flotte aux seins mais ça moule le ventre, c’est un enfer, ces tailles standardisées, ça n’existe que pour un type de physique qui est malheureusement imaginaire, et en ce qui concerne le corps des femmes qui varie très fort d’un individu à l’autre, ça oblige à cliver le monde entre taille normale et grande taille, ce qui est super pour l’ego, d’autant que les coupes grandes tailles consistent souvent à recouvrir le corps concerné pour le faire disparaître le plus possible au regard.
On voudrait bien pouvoir se faire un vêtement soi-même, durable, joli, et dans lequel on se sent parfaitement bien, seulement tout le monde n’est pas en mesure de tracer soi-même son patron, c’est une compétence qui demande tout de même un certain nombre de connaissances et qui prend du temps à mettre en œuvre.
Il y a bien une offre de patrons de couture, mais qui eux aussi correspondent à des tailles normalisées. C’est pas vraiment de la faute aux fournisseurs. Avant l’avènement du logiciel, tous les patrons étaient tracés manuellement, c’était impossible d’anticiper chaque variation corporelle. Mais l’informatique change beaucoup la donne : grâce aux logiciels de modélisme de mode, on peut tracer un patron en lui donnant suffisamment de repères (c’est le principe de la conception paramétrique ou CAD) pour qu’il s’adapte tout seul quand les variables désignées changent (par exemple, les tailles).
Seulement la formation de modélisme de mode et celle de design paramétrique c’est encore deux choses distinctes, et la plupart des concepteurs se contentent d’exploiter les extraordinaires possibilités de ces programmes pour se simplifier le travail de gradation (porter le même patron dans plusieurs tailles différentes)
Moi, ce que je voulais, c’est un patron qui ne se contente pas d’augmenter ou de diminuer de taille, mais où tout se modifierait en fonction d’un seul paramètre modifié. Ton tour de bras augmente? Hop, tu le rentres dans un formulaire, et tout le patron suit : le dessin complet de la manche, de l’emmanchure, les pinces, etc.
Il existe déjà des initiatives qui vont dans ce sens : freesewing propose une série de patrons intégralement gratuits avec un éditeur en ligne, barapatron , sewist CAD ou paternette par exemple sont autant de shops qui se proposent de générer un patron selon vos désirs.
C’est pas pour taper sur la concurrence, mais aucune de ces solutions n’a rencontré mes grâces. Dans le cas des shops, parce que la plupart se contentent de combiner différents designs et de baser leurs déclinaisons sur le principe de la gradation encore une fois (donc y a un tableau des tailles, en clair…)
Dans le cas de freesewing, parce que d’une part la quantité de modèles est limitée et pensée d’une façon particulière (on voit que les designs ont été créés avec la simplicité en tête pour s’assurer du taux de réussite du modèle) et d’autre part parce que la plupart… finissent par casser.
J’en suis venue à cette idée de patron paramétrique avec une raison bien précise en tête : j’avais une pathologie aux deux bras qui les a rendus très volumineux. Plus la maladie avançait, plus il m’était difficile de trouver des vêtements portables. Au dernier stade que j’ai connu, c’en était devenu ridicule : il aurait fallu que les manches de mon manteau soient en taille 60 ou plus, quand mon tour de taille était en 40 ou 42 tout au plus. Je te raconte pas les hivers dans ma montagne où il y avait du blizzard et on tapait parfois dans les -20 degrés Celsius, avec mes bras engoncés, mes mouvements limités, et le vent et la glace qui s’engouffraient contre mon ventre. Et impossible de trouver plus performant qu’une veste de bûcheron à carreaux doublé de pilou synthétique pour y caser mes invraisemblables œdèmes. Ça aurait même fini par constituer un risque, à la longue, et ça n’avait pas un effet merveilleux sur ma psyché de m’emballer dans des fringues quatre ou cinq fois trop grandes.
Donc il fallait que je me résigne à retourner à tracer mes patrons, ce que je savais faire mais n’avais plus fait depuis une petite éternité. Et connaissant par ailleurs le design paramétrique via le design d’objets pour l’impression 3D ou la découpe CNC, j’étais sûre qu’il y avait de quoi se faciliter considérablement la vie. C’est en cherchant dans ce sens que je suis tombée sur freesewing, où j’ai religieusement pété les limites de tous les designs avec ma circonférence de bras insensée. J’aurais bien pu contribuer à créer des designs davantage paramétriques pour cette plateforme, vu que c’est open source et basé sur la contribution, mais je n’ai malheureusement pas les connaissances pour la partie « patterns as code » et si j’ai cassé tous les modèles de la plateforme avec un seul paramètre, c’est peut-être que leur logiciel a cette limite qui ne me permettrait pas de réaliser ce que j’ai en tête.
Et donc j’ai continué à explorer mes possibilités et je suis tombée sur Seamly2d, un logiciel FOSS (gratuit et open source) forké de Valentina, de dessin paramétrique destiné spécifiquement au modélisme de mode, qui prend en charge suffisamment de fonctions trigonométriques pour permettre de contraindre intégralement un modèle avec beaucoup de souplesse. Je me suis donc basée sur ce support pour élaborer un bloc de base – le patron duquel découlent tous les patrons – qu’idéalement je voudrais universel et à partir duquel on pourrait, justement, développer tous les patrons. Et comme je me suis dit que les femmes, en population diverse et hétéroclite, doivent être nombreuses à rencontrer des problèmes similaires ou du moins analogues au miens, j’ai récolté un max de mensurations de contributrices anonymes et je me suis mise au boulot.
J’ai dû mettre ce projet en pause, ironiquement, parce que je me suis enfin fait opérer des bras, desquels on a sorti plus de six kilos de tissu néoplasique et fibrosé, des nodules, un cauchemar, troqué contre deux cicatrices qui vont de l’aisselle au coude et que je trouve plutôt badass, et à l’instant où je peux enfin me resservir correctement de mes bras et amener ce projet à son terme, l’oedème a fini de se résorber et je peux de nouveau renter mes petits bras de poulet dans toutes les coquettes petites merdes de prêt-à-porter qui me faisaient tellement déprimer il y a encore quelques semaines.
Heureusement, ou malheureusement, ce projet reste pertinent puisqu’il peut potentiellement répondre à d’autres problématiques de corps non-standards, et présenter une alternative viable et bon marché à certains vêtements spécialisés – d’ailleurs le patron de juste après (le fameux patron secret, pour ceux qui ont suivi) a aussi une destination utilitaire et sera également gratuit.
J’ai terminé le patron avant-hier, il est en ligne sur mon shop ko-fi où je trouve important de le mettre à disposition gratuitement (votre contribution volontaire à mon travail est bien sûr bienvenue) et où vous pouvez le trouver en plusieurs saveurs : tailles normalisées du 34 au 60 pour ceux qui voilà hein voudraient juste un patron gratos pour se faire un fond de base et / ou se fabriquer un mannequin sans trop s’emmerder, sur mesure à la demande contre une petite contribution pour ceux qui n’ont pas envie de mettre les mains dans le cambouis, et les fichiers à utiliser directement dans Seamly pour les bricoleurs qui ont envie de le mettre à leur taille et d’itérer directement à partir de là. Le tout est sous licence CC-BY SA, ce qui signifie que si vous souhaitez vendre un vêtement produit à partir de ce matériel, vous y êtes autorisé, à condition de créditer mon travail.
Patron bloc de base universel femme by Morayner’s Workshop is licensed under Creative Commons Attribution-ShareAlike 4.0 International
Pour cette dernière option, on va filer dans un autre article dans la section tutos & templates où je ne vais pas vous donner un cours complet d’utilisation du logiciel, mais vous montrer vite fait comment importer vos mesures sur le patron et comment exporter le patron à vos mesures pour l’imprimer et l’assembler.
Ah oui, dernier détail : Je me suis attaquée en premier au patron pour les femmes parce que pour une fois ça fait pas de mal que les femmes passent en premier quelque part – et aussi accessoirement parce que j’en suis une – mais un patron homme est prévu, il arrivera tôt ou tard.
Merci pour ta lecture, des bisous, et à tout de suite dans le tuto! Tu peux le trouver ici
Si tu veux soutenir mon travail, tu peux me payer un café ici Merci beaucoup!
https://morayner.org/un-patron-numerique-sur-mesure-1-le-bloc-de-base/
#CAD #couture #handicap #parametricDesign #patronDeCouture #seamly2d
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Edit: C'est bon, j'ai une solution ! Merci beaucoup !
Appel à la générosité, je cherche une loupe binoculaire (utilisée entre autre pour souder les petits composants, ou dans les laboratoires).
Je fais rarement ce type d'appel, mais malheureusement, l'État belge a décidé de réduire fortement les aides au handicap. J'ai une acuité visuelle faible, un trentième environ. Il me faut plus de 5mn pour lire la plus grosse des lettres lors des tests ophtalmologiques. Autrement dit, il me faut grossir énormément les choses pour bien voir.
Et en parallèle de ça, je fais du prototypage de choses en DIY. J'ai créé des jeux de go accessibles aux personnes déficientes visuelles. Et rien que cet été, j'en ai imprimé quelques-uns, ainsi qu'une grande quantité de dés de JDR. Je fais un peu de couture, j'essaie de souder des composants traversants sur des plaques électroniques (mais ça ne marche pas vraiment, je ne vois pas le résultat final, c'est trop compliqué !). Et puis j'ai besoin de faire du post-traitement sur mes impressions 3D. Enfin bref, je bricole beaucoup de petites choses sur table. Mon prochain objectif, ce sera de proposer un kit de mise en accessibilité du jeu de rôle convenant au maximum de situations de handicap combinées : autisme, aphantasie, POC, cécité, TDAH, etc. Combiner les handicaps autour d'une table est d'une complexité infernale, et il faut des outils adaptés.
Et avec la réduction des aides, il va falloir penser aussi à préparer notre avenir, quand on ne pourra strictement plus rien se payer pour rendre notre quotidien adapté. Je compte tenter de concevoir quelques aides pour pallier ça.
Malheureusement, une simple caméra connectée à un écran a un certain lag, et ça devient dangereux ou compliqué quand je manipule des éléments toxiques ou chauds. Je ne peux pas envisager ce type de matériel pour cet usage.
Si vous disposez de ce type de matériel et que vous le donnez, je serais très intéressé, et reconnaissant ! Je peux participer aux frais de port, et dédommager un peu, à hauteur de 200 euros maximum. On peut faire le déplacement pour récupérer le matériel sur toute la #Belgique, sud des #Pays-Bas, #Luxembourg, autour de #Lille et même au-delà.
Un tout grand merci pour votre soutien et votre partage !
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A new framework, consisting of seven components, is proposed for evaluating the trustworthiness of scientific research. Some components have a quantitative basis but need criteria to be established; others are not so readily quantified or assessed.
Original paper: https://www.pnas.org/doi/10.1073/pnas.2536736123
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Research and advocacy for a simplified FAFSA becomes reality
Early in her career, Professor Susan Dynarski set her sights on simplifying the Free Application for Federal Student Aid (#FAFSA), a form any student must file to receive a federal loan or Pell grant.
She and colleague Judith Scott-Clayton first quantified the unnecessary complexity of the FAFSA in a 2006 paper. They found that if you asked just four questions—including one on income and another on family structure—the answers could explain 90 percent of the variation in financial aid eligibility.
“Literally the thing that had the greatest impact was showing that there were more questions in the FAFSA than in the 1040 Tax form,” said Dynarski in a 2018 interviewl.Since 2006, Dynarski has revisited the research, written opinion articles, and testified before Congress about her findings.
Lamar Alexander, the now-retired Republican U.S. Senator from Tennessee, and former U.S. Secretary of Education took notice. Citing Dynarski’s research, Alexander made the case “to reduce the federal financial aid application to a form the size of a postcard.” He introduced a bill and then continued to push for legislation in the following years to simplify the form.
In the end, the FAFSA will be a bit longer than Dynarski or Alexander’s ideal, but it will still be shortened significantly.
Starting in 2023, the FAFSA will include just 33 questions from the original 108, and applicants will also be able to directly transfer tax information from the IRS.
👏👍#fafsa #studentaid #governmentforms #accessible #streamline #simplify 💪💕
https://fordschool.umich.edu/news/2021/dynarskis-research-and-advocacy-simplified-fafsa-becomes-reality -
CW: Using Human Pluripotent Stem Cells to Create Human Skeletal Muscle Organoids for Repair and Regeneration
Skeletal #muscle is a type of tissue that makes up a large part of the human body. It is made up of many different cells that are able to contract and move. Skeletal muscle has the ability to #repair itself when it is damaged due to #aging, exercise, or diseases like #MuscularDystrophy. A small group of cells called #SatelliteCell s help with the repair process. Scientists have been trying to create models to study how #Skeletalmuscle develops and regenerates. Recently, they have been using human pluripotent #StemCell to create 3D models of skeletal muscle tissue. However, these models have not been able to recreate the full process of muscle regeneration. In this research paper, the authors introduce a new method of using human pluripotent stem cells to create 3D models of skeletal muscle tissue that can retain the ability to repair itself.
Over the past decades, scientists have used #animalmodel to study #muscleregeneration, which is regulated by #stemcell s. These animal models have been very helpful in understanding the mechanisms of muscle #regeneration, but they don't always accurately reflect the same range of diseases that humans experience. Therefore, researchers have suggested creating reliable in vitro models using human muscle cells. ( #hPSC s) could be used to create 3D human skeletal muscle #organoid s ( #hSkMO s) that contain sustainable #stemcell and distinct myofibers with the same proteins and structure as adult muscles. Previous approaches to skeletal muscle differentiation have been developed using 2D #culture systems, but these lack the natural environment and #StemCell niche that are necessary to model adult #myogenesis and muscle #regeneration.
#Stemcell s ( #SC s) can be used to repair damaged muscle tissue. They explain that SCs can be activated in response to muscle injuries and that other #cell types can contribute to the process of #myogenesis. The author then goes on to explain that #cytokine s, such as IL-4, can influence the #InflammatorySystem and promote SCs differentiation, which helps with muscle regeneration. While #organoid s generated from #hPSC s have potential, they do not fully replicate the in vivo native microenvironment. To address this, treat the #hSkMO s with extrinsic #cytokine s to promote #muscle #regeneration . #hSkMO s might then be used to study aspects of human muscle #biology and to identify novel #therapeutic candidates for muscle-wasting disorders.
To create a 3D structure of muscle tissue. They used #WNT activator and #BMP inhibitors at the beginning of the differentiation process to induce paraxial #mesodermal #cell s. They then added #FGF2 to the Matrigel to promote the 3D structure. #HGF and IGF1 were added later to accelerate the #myogenic specification and further #myofiber differentiation. They optimized the timing of the Matrigel embedding to day seven. After this, they observed #neuralcell s and withdrew FGF2 to focus on muscle tissue development. They then prolonged the HGF and IGF1 treatment to propagate #myogenic #progenitor s. They found that 62% of the #tissue was #skeletalmuscle tissue and that it contained PAX7+ #myogenic #stem / #progenitor cells, MYOD+ activated/committed #myoblast s, and MYOG+ #myocyte s. They also found that 31% of PAX7+/Ki67− and 29% of MYOD−/PAX7+ non-dividing quiescent SCs were present in the mature #hSkMO s. This indicates that the #hSkMO s were able to effectively recreate #embryo nic #myogenesis and have regenerative potential. Future studies using #singlecell #RNA sequencing may be necessary to further characterize the different types of cells in #hSkMO s.
The stepwise process to generate human skeletal muscle organoid s (hSkMOs) from human pluripotent stem cells (hPSCs)
The process begins with dissociating #hPSC s into #singlecell s and allowing them to form #embryoid bodies ( #EB s) in low-attachment V-shaped 96-well plates. Then, paraxial #mesodermal differentiation is promoted with #WNT activation, BMP inhibition, and FGF2 signaling. The expression of pluripotency markers OCT4 and NANOG decreases, and the expression of #mesoderm markers Brachyury, T-Box transcription factor 6 (TBX6), and mesogenin 1 (MSGN1) increases. To further characterize paraxial #mesoderm al differentiation, TBX6 is #immunostain ed. After paraxial #mesodermal induction, the #organoid s are embedded with growth factor-reduced Matrigel and transferred to a six-well plate on an orbital shaker. Growth factors are then added to the #myogenic specification media, and #hSkMO s are cultured until the day of analysis. The orbital shaker improves the viability, survival, and differentiation of hSkMOs by increasing the penetration rate of oxygen and nutrients into the core area of hSkMOs. The #hSkMOs gradually grow to more than 1.5 mm in diameter by day 60, appearing round-shaped, uniformly sized, and having relatively homogenous morphology. PAX3 and PAX7 are #myogenic progenitor markers, and their expression is verified by qRT-PCR and #cryo sections. The #myogenic cells appear as clusters, and approximately 9% of PAX7+ cells are double-positive for Ki67 at day 30, demonstrating that proliferating cells are #myogenic #progenitor s in hSkMOs. This indicates that the in vitro #hSkMO #culturesystem is able to recapitulate the features of embryonic skeletal #muscle development.
The different types of #SkeletalMuscle stem/progenitor cells that are involved in myogenesis, the process of muscle formation.
The researchers used qRT-PCR analysis and #immunohistochemistry to identify and characterize the different types of cells. They found that PAX3 and PAX7 (SC markers) were the major population during the early stage of #myogenesis, and that MYOD (proliferating and activated SC marker) and MYOG (differentiated myocyte marker) increased over time. They also observed that MYOD−/PAX7+, MYOD+/PAX7+, and MYOD+/Ki67+ cells accounted for 29%, 6%, and 8% of the putative quiescent, activated, and proliferating #SC s, respectively. MYOD+/PAX7− cells constituted 39% of differentiating myoblasts, and MYOG−/PAX7+ cells constituted 23% of putative quiescent SCs. MYOG+/PAX7− cells accounted for 30% of differentiated #myocyte s, and 8% and 6% of the MYOG+ cells in #hSkMO s co-expressed PAX7 and Ki67, respectively. This data shows that the researchers were able to identify and characterize different types of skeletal muscle stem/progenitor cells during #myogenesis.
The text is discussing the results of a research study that used hSkMOs (human skeletal muscle #organoid s) to study the development of skeletal muscle #tissue. The study found that the #hSkMO s grew exponentially in size within two months, and the growth rate then steadily decreased. The researchers then used scanning electron microscopy (SEM) imaging and confocal microscopy to examine the cytoarchitecture of the hSkMOs. They found that the hSkMOs contained a large population of terminally differentiated #myogenic cells and a small population of preserved myogenic stem/progenitor cells. They also found that the hSkMOs contained a substantial proportion of TITIN+ muscle cells and MAP2-positive #neuron s. To further characterize the presence of sustainable stem cells within the mature hSkMOs, they quantified the amount of dormant stem cells by #confocal #microscopy imaging. The results showed that approximately 56%, 31%, and 5% of PAX7+/Ki67- putative dormant stem cells existed throughout the differentiation of hSkMOs at days 30, 70, and 130, respectively. This indicates that the hSkMOs contained mature skeletal muscle properties and had the potential for #regeneration .
The researchers wanted to see if the #hSkMO s (human #skeletal muscle #organoid s) had the ability to regenerate #muscle #tissue after damage. To test this, they treated the hSkMOs with a cardiotoxin (CTX) which is known to induce muscle inflammation and damage. They then observed a decrease in PAX7+ and MYOD+ cells in the hSkMOs. To further test the #regenerative potential of the #hSkMO s, they added interleukin-4 (IL-4) to the medium to promote #muscleregeneration. After 14 days, they observed a significant increase in MYOG+ myocytes in the CTX-injured hSkMOs with the treatment of IL-4 compared to the CTX-injured hSkMOs without the treatment. This suggests that the hSkMOs have the potential to regenerate muscle tissue after damage.
Generation of Skeletal Muscle Organoids from Human Pluripotent Stem Cells to Model Myogenesis and Muscle Regeneration
Authors :
Min-Kyoung Shin , Jin Seok Bang , Jeoung Eun Lee , Hoang-Dai Tran , Genehong Park , Dong Ryul Lee and Junghyun Jo
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(18/24) ... abattu vol #MH17 Malaysia Airlines assassinant 298 personnes à bord.
- Inde : usine embouteillage Coca Cola Uttar Pradesh fermée par Justice nouvelles accusée extraire quantité excessive #eau nappe phréatique 300 000 l eau/jour (cf 1...) -> Coca fait appel car investissement futur 5 milliards $ jusqu'en 2020 + puits asséchés pompes à main ...#year2014 #anthropocene #climat #climate #climatechange #climatecrisis #art #photography #photo #NickBrandt
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Du plomb en quantité dans les écoles parisiennes, mais un suivi de la mairie défaillant
Depuis le scandale sanitaire qui a suivi l’incendie de Notre-Dame, la ville de Paris contrôle la pollution au #plomb en milieu scolaire. Mais sans toujours prendre les mesures nécessaires face à des résultats parfois alarmants. Plusieurs cas de saturnisme posent question.
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Conditional deep generative models as surrogates for spatial field solution reconstruction with quantified uncertainty in Structural Health Monitoring applications
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L'enseignement ressemble beaucoup à ce qu'Ellul appelle la "pré-propagande" - le conditionnement des esprits avec de vastes quantités d'informations incohérentes, déjà diffusées à des fins inavouées et présentées comme des "faits" et de "l'enseignement". Ellul poursuit en désignant les intellectuel·les comme étant virtuellement les plus vulnérables de tou·tes à la propagande moderne, et ce pour trois raisons :
1. iels absorbent la plus grande quantité d'informations de seconde main, invérifiables ;
2. iels ressentent le besoin impérieux d'avoir une opinion sur toutes les questions importantes de notre époque, et succombent donc facilement aux opinions que leur propose la propagande sur toutes ces informations indigestes ;
3. iels s'estiment capables de "juger par soi-même". Iels ont littéralement besoin de la propagande.
En fait, le besoin de propagande de la part du "propagandé" est l'un des éléments les plus puissants de la thèse d'Ellul.(en) https://archive.org/details/propaganda-jacques-ellul
#enseignement #propagande #Ellul #philosophie #éthique #confiance #inclusion #sureté #sociologie #conformisme #intellectuels #université #postFactuel
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Au passage, ces deux entreprises américaines que les banques vous imposent pour établir votre cote de crédit et qui collectent d’immenses quantité de données personnelles sur vous devraient être interdites. #transunion
https://mastodon.social/@WarnerCrocker/115106308835835022 -
CW: ptpol debates IL x CDU
5) #salários: IL deixou cair a subida do salário médio no programa. CDU quer SMN 1000€ em Julho.
6) #economia: CDU quer aumentar produção nacional, diversificar relações comerciais e mercado interno. Não ir ao beija-mão ao #Trump. É preciso ajudar sectores mais prejudicados (ex. vinho). Vem aí um défice diz a jornalista, mas IL quer investir na defesa sem fazer cortes, fazendo crescer a economia (rejeitando a ideia de que vem aí défice) baixando impostos.
As últimas palavras que se ouvem neste debate são de #Raimundo a dizer "Fica-lhe muito mal acabar o debate nestes termos, Rui Rocha." Porquê? Bem, Rocha ao ver que o debate estava a chegar ao fim, deixou a sua resposta sobre a economia a meio para debitar uma quantidade de acusações ao #PCP sobre a #Ucrânia não dado espaço a Raimindo para as rebater.
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Amazon a lancé #RxPass, un abonnement (aux USA), qui permet pour 5 $ par mois de se procurer des médicaments génériques sur ordonnance en quantité illimitée et sans frais supplémentaires https://www.bfmtv.com/economie/entreprises/amazon-propose-des-medicaments-generiques-en-illimite-pour-5-dollars-par-mois_AD-202301240583.html (BFMTV) https://www.bfmtv.com/economie/entreprises/amazon-propose-des-medicaments-generiques-en-illimite-pour-5-dollars-par-mois_AD-202301240583.html
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TikTok vai permitir reduzir a quantidade de vídeos gerados por IA no seu feed
🔗 https://tugatech.com.pt/t74496-tiktok-vai-permitir-reduzir-a-quantidade-de-videos-gerados-por-ia-no-seu-feed#artificial #computador #comunidade #controlo #empresa #extra #falha #feed #ferramenta #funcionalidade #ia #objetivo #olho #origem #os #pinterest #plataforma #rede #sistema #social #tecnologia #tiktok
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- "La vérité, c'est qu'il y a une quantité impressionnantes de gouttes qui ne font pas déborder le vase.", Romain Gary
- photo : William Cheh -
"Le système algébrique des symboles est un calcul, c'est-à-dire un langage pour raisonner. Par conséquent, s'il est parfaitement approprié de définir une dette comme un bien négatif, expliquer ce qu'est une quantité négative en disant qu'elle est ce qu'est une dette à un bien, c'est mettre la charrue avant les bœufs, et expliquer le plus intelligible par le moins intelligible. [...]" – Charles Sanders Peirce (1839-1914)
#citation #mathématiques #maths #math -
CW: Alcohol // Alcool
The Liquor Control Board of Ontario refuses to publish the quantity of alcohol they have in their inventory, citing “cabinet confidence”
https://www.cbc.ca/news/canada/ontario-lcbo-us-alcohol-inventory-9.6961672
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LCBO refuse de publier la quantité d'alcool qu'il a dans son inventaire, invoquant la « confiance du cabinet »// Article en anglais //
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#Réchauffement #climatique «12 #bombes #atomiques explosant #chaque #seconde de #chaque #jour de l’année» : les #océans ont absorbé une #quantité #record de #chaleur en 2025 www.liberation.fr/environnemen...
«12 bombes atomiques explosant... -
CW: Taxation
Cities covering the cost of social services is being labeled as “bad news.”
That label is doing a lot of ideological work.
In reality, this may be the beginning of a long-overdue shift: moving the cost of running a city away from workers, and toward those who own the land.
This shift is good news, not bad news!
https://www.cbc.ca/news/canada/british-columbia/bc-municipal-downloading-quantified-9.7032840
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CW: Taxation
Cities covering the cost of social services is being labeled as “bad news.”
That label is doing a lot of ideological work.
In reality, this may be the beginning of a long-overdue shift: moving the cost of running a city away from workers, and toward those who own the land.
This shift is good news, not bad news!
https://www.cbc.ca/news/canada/british-columbia/bc-municipal-downloading-quantified-9.7032840
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CW: Taxation
Cities covering the cost of social services is being labeled as “bad news.”
That label is doing a lot of ideological work.
In reality, this may be the beginning of a long-overdue shift: moving the cost of running a city away from workers, and toward those who own the land.
This shift is good news, not bad news!
https://www.cbc.ca/news/canada/british-columbia/bc-municipal-downloading-quantified-9.7032840
-
CW: Taxation
Cities covering the cost of social services is being labeled as “bad news.”
That label is doing a lot of ideological work.
In reality, this may be the beginning of a long-overdue shift: moving the cost of running a city away from workers, and toward those who own the land.
This shift is good news, not bad news!
https://www.cbc.ca/news/canada/british-columbia/bc-municipal-downloading-quantified-9.7032840