#singlemolecule — Public Fediverse posts

Does anyone have strong opinions on what the best/most sensitive low volume spectrophotometer is? Looking to quantify low concentrations of fluorescently labelled protein, not just DNA etc

#biochem #biophys #singlemolecule

Postdoc Fellow - Single-molecule biophysics at 2D material interfaces

TU Delft

Call for postdocs: Do you enjoy #nanoscience, #singlemolecule techniques and #2Dmaterials research? Check out our #ERC postdoc vacancy at #TUDelft

See the full job description on jobRxiv: https://jobrxiv.org/job/tu-delft-27778-postdoc-fellow-single-molecule-biophysics-at-2d-material-interfac...
https://jobrxiv.org/job/tu-delft-27778-postdoc-fellow-single-molecule-biophysics-at-2d-material-interfaces/?feed_id=79932

Our work has made the inside cover! Check out "#singlemolecule #electrochemical imaging resolves the midpoint potentials of individual fluorophores on nanoporous antimony-doped tin oxide" in the latest issue of @chemicalscience! https://doi.org/10.1039/d3sc05293a @washuengineers #WashUESE #WUSTL

Congratulations to Prof. Jin Lu, now at the National Center for Nanoscience, whose work on #singlemolecule #electrochemical imaging is now online in Chemical Science. Happy New Year!
#SMLM #WashU #WUSTL @chemicalscience #ChemicalScience

"Single-Molecule Electrochemical Imaging Resolves the Midpoint Potentials of Individual Fluorophores on Nanoporous Antimony-Doped Tin Oxide"
Chemical Science, 2024
https://doi.org/10.1039/D3SC05293A

new citation:

`Using salt tolerance compartmentalized self-replication (stCSR) and a #microfluidic platform, we obtained 11 mutant sites with enhanced salt tolerance attributes. #Sequencing and #biochemical analyses revealed that the substitution of conserved amino acids such as G197D, Y369E, T372N, and I378R plays a critical role in maintaining the #processivity of exonuclease-deficient #phi29 #polymerase under high salt conditions.`

https://www.frontiersin.org/articles/10.3389/fmicb.2023.1267196/full

#nanopore #DNA #singleMolecule

Join us for @TingtingWu 's PhD defense, "Information-Rich #6D #SingleMolecule Imaging Using Optimization," tomorrow at 1 PM CST in Rodin Auditorium! https://happenings.wustl.edu/event/ese_defense_tingting_wu #WashUESE #wuimgsci_stuco #wustlspectra #WashUengineers

"The mechanism of mRNA activation"
A preprint looking at the role of eIF4F in translation initiation at single molecule resolution.
#mRNA #translation #ribosome #singlemolecule #microscopy

https://www.biorxiv.org/content/10.1101/2023.11.15.567265v1?rss=1

"By controlling the distance between the #nanopore and glass surface, we can actively select the region of interest on the molecule and scan it a controlled number of times and at a controlled velocity. Decreasing the velocity and averaging thousands of consecutive readings of the same molecule increases the signal-to-noise ratio by two orders of magnitude compared with free translocations."

https://www.nature.com/articles/s41565-023-01412-4

#DNA #singleMolecule #biosensor

Our NIH #R35 #MIRA has been awarded! I'm glad to be continuing collaborations within #WashU #McKelveyEngineering, and pushing our development of #6D #singlemolecule #nanoscopy.

https://engineering.wustl.edu/news/2023/Looking-deeper-with-adaptive-six-dimensional-nanoscopy.html

Our NIH #R35 #MIRA has been awarded! I'm glad to be continuing collaborations within #WashU #McKelveyEngineering, and pushing our development of #6D #singlemolecule #nanoscopy.

https://engineering.wustl.edu/news/2023/Looking-deeper-with-adaptive-six-dimensional-nanoscopy.html

Our NIH #R35 #MIRA has been awarded! I'm glad to be continuing collaborations within #WashU #McKelveyEngineering, and pushing our development of #6D #singlemolecule #nanoscopy.

https://engineering.wustl.edu/news/2023/Looking-deeper-with-adaptive-six-dimensional-nanoscopy.html

Our NIH #R35 #MIRA has been awarded! I'm glad to be continuing collaborations within #WashU #McKelveyEngineering, and pushing our development of #6D #singlemolecule #nanoscopy.

https://engineering.wustl.edu/news/2023/Looking-deeper-with-adaptive-six-dimensional-nanoscopy.html

Super-cool work from our clever colleagues at Cambridge -- whole-cell #3D #singlemolecule #lightfield imaging! #SMLM

@samofmidworld

High-density volumetric super-resolution microscopy
bioRxiv 2023.05.02.539032;
doi: https://doi.org/10.1101/2023.05.02.539032

https://www.nature.com/articles/s41587-023-01743-6

"We show that default nanopore sequencing settings discard the vast majority of tRNA reads, leading to poor sequencing yields and biased representations of tRNA abundances based on their transcript length. Re-processing of raw nanopore current intensity signals leads to a 12-fold increase in the number of recovered tRNA reads and enables recapitulation of accurate tRNA abundances"

#transferRNA #tRNA #nanopore #sequencing #singleMolecule #biosensor #nanosensor #nanotech

Excited to share that our #6D #singlemolecule #superresolution #microscope, which we uses a multi-view reflector architecture, is now online in #NaturePhotonics! Congratulations #oumeng_zhang! https://doi.org/10.1038/s41566

A big thanks to Michael Vahey's group in #WashUBME
#WashUengineers #WashU

Full-text access here: https://rdcu.be/c02le