#singlemolecule — Public Fediverse posts
Live and recent posts from across the Fediverse tagged #singlemolecule, aggregated by home.social.
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Our preprint is up! Take a look if you like kinesin, microtubule transport, single molecule FRET or biophysics. It’s a bit of shift to how we’ve been used to thinking about kinesin-1 regulation as we try to get a handle on its conformational dynamics. Super proud of to have this milestone out in the world and all the hard work it represents from everyone involved.
#kinesin #microtubules #biophys #smFRET #SingleMolecule #AxonalTransport https://biologists.social/@TwelvetreesLab/113691748206473977 -
Does anyone have strong opinions on what the best/most sensitive low volume spectrophotometer is? Looking to quantify low concentrations of fluorescently labelled protein, not just DNA etc
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Postdoc Fellow - Single-molecule biophysics at 2D material interfaces
TU Delft
Call for postdocs: Do you enjoy #nanoscience, #singlemolecule techniques and #2Dmaterials research? Check out our #ERC postdoc vacancy at #TUDelft
See the full job description on jobRxiv: https://jobrxiv.org/job/tu-delft-27778-postdoc-fellow-single-molecule-biophysics-at-2d-material-interfac...
https://jobrxiv.org/job/tu-delft-27778-postdoc-fellow-single-molecule-biophysics-at-2d-material-interfaces/?feed_id=79932 -
Our work has made the inside cover! Check out "#singlemolecule #electrochemical imaging resolves the midpoint potentials of individual fluorophores on nanoporous antimony-doped tin oxide" in the latest issue of @chemicalscience! https://doi.org/10.1039/d3sc05293a @washuengineers #WashUESE #WUSTL
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Congratulations to Prof. Jin Lu, now at the National Center for Nanoscience, whose work on #singlemolecule #electrochemical imaging is now online in Chemical Science. Happy New Year!
#SMLM #WashU #WUSTL @chemicalscience #ChemicalScience"Single-Molecule Electrochemical Imaging Resolves the Midpoint Potentials of Individual Fluorophores on Nanoporous Antimony-Doped Tin Oxide"
Chemical Science, 2024
https://doi.org/10.1039/D3SC05293A -
Great to hear W.E. on the Master Minds podcast with wisdom for young scientists and engineers! https://www.mastermindspod.com/episode-gallery/episode/e039f3bb/episode-23-peering-into-the-world-of-single-molecules-with-prof-we-moerner-phd #SingleMolecule @washuengineers
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new citation:
`Using salt tolerance compartmentalized self-replication (stCSR) and a #microfluidic platform, we obtained 11 mutant sites with enhanced salt tolerance attributes. #Sequencing and #biochemical analyses revealed that the substitution of conserved amino acids such as G197D, Y369E, T372N, and I378R plays a critical role in maintaining the #processivity of exonuclease-deficient #phi29 #polymerase under high salt conditions.`
https://www.frontiersin.org/articles/10.3389/fmicb.2023.1267196/full
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Join us for @TingtingWu 's PhD defense, "Information-Rich #6D #SingleMolecule Imaging Using Optimization," tomorrow at 1 PM CST in Rodin Auditorium! https://happenings.wustl.edu/event/ese_defense_tingting_wu #WashUESE #wuimgsci_stuco #wustlspectra #WashUengineers
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"The mechanism of mRNA activation"
A preprint looking at the role of eIF4F in translation initiation at single molecule resolution.
#mRNA #translation #ribosome #singlemolecule #microscopyhttps://www.biorxiv.org/content/10.1101/2023.11.15.567265v1?rss=1
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🎯🐠 I've been away for a while. But here:
I wrote a blog post on "Imaging mRNA modifications in situ"Featuring: m6AISH-PLA, ARPLA (on #glycoRNA) & DART-FISH.
#smFISH #m6A #RNA #mRNA #RNAmodifications #microscopy #imaging #singlemolecule
https://greenfluorescentblog.wordpress.com/2023/10/31/imaging-mrna-modifications-in-situ/
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Iva Lučić shows our students how to label CaMKII with fluorescent dye for #singleMolecule TIRF #microscopy #biophysics
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This is really cool- great to see #singlemolecule *molecular* scale behaviour inside #condensates: https://www.nature.com/articles/s41586-023-06329-5
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Hello! A new intro for a new instance.
I lead a research group trying to figure out axonal transport and neurodegeneration. A big emphasis on the microtubules and their motors, but really the transport of anything that doesn't have a membrane around it. For this we use a lot of single molecule methods and live cell imaging. I also post about research culture.
#introduction #kinesin #dynein #microtubules #neurodegeneration #AxonalTransport #neurobiology #SingleMolecule #CellBiology
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"By controlling the distance between the #nanopore and glass surface, we can actively select the region of interest on the molecule and scan it a controlled number of times and at a controlled velocity. Decreasing the velocity and averaging thousands of consecutive readings of the same molecule increases the signal-to-noise ratio by two orders of magnitude compared with free translocations."
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🔬 What are the next advancements in the field of #singlemolecule sequencing technologies? We talked about it with Francesco Nicassio (Istituto Italiano di Tecnologia)
“Only together we can really find new solutions”
#science #sciencemastodon #lifesciences #humantechnopole https://www.youtube.com/watch?v=JihuHbYmdYk
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Our NIH #R35 #MIRA has been awarded! I'm glad to be continuing collaborations within #WashU #McKelveyEngineering, and pushing our development of #6D #singlemolecule #nanoscopy.
https://engineering.wustl.edu/news/2023/Looking-deeper-with-adaptive-six-dimensional-nanoscopy.html
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Super-cool work from our clever colleagues at Cambridge -- whole-cell #3D #singlemolecule #lightfield imaging! #SMLM
High-density volumetric super-resolution microscopy
bioRxiv 2023.05.02.539032;
doi: https://doi.org/10.1101/2023.05.02.539032 -
PhD Position - Acoustofluidic Single-molecule Nanosystems
#PhDvacancy on #nanodevices and #singlemolecule biophysics. Join our interdisciplinary team at #TUDelft in 2023!
#ScienceJobs #job
Delft #Netherlands #PhDStudent
https://jobrxiv.org/job/tu-delft-27778-phd-position-acoustofluidic-single-molecule-nanosystems/?feed_id=44208 -
🔬 How can single molecule sequencing technologies change research on the human genome? Listen to Davide Bolognini, Senior Bioinformatician in our Genomics Research Centre.
“International collaborations, of course, are an essential part for developing new methods”
#lifesciences #humantechnopole #genomics #singlemolecule #sequencing #humangenome #science #sciencemastodon
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PhD Position - Acoustofluidic Single-molecule Nanosystems
#PhDvacancy on #nanodevices and #singlemolecule biophysics. Join our interdisciplinary team at #TUDelft in 2023!
#ScienceJobs #job
Delft #Netherlands #PhDStudent
https://jobrxiv.org/job/tu-delft-27778-phd-position-acoustofluidic-single-molecule-nanosystems/?feed_id=42570 -
https://www.nature.com/articles/s41587-023-01743-6
"We show that default nanopore sequencing settings discard the vast majority of tRNA reads, leading to poor sequencing yields and biased representations of tRNA abundances based on their transcript length. Re-processing of raw nanopore current intensity signals leads to a 12-fold increase in the number of recovered tRNA reads and enables recapitulation of accurate tRNA abundances"
#transferRNA #tRNA #nanopore #sequencing #singleMolecule #biosensor #nanosensor #nanotech
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PhD Position - Acoustofluidic Single-molecule Nanosystems
#PhDvacancy on #nanodevices and #singlemolecule biophysics. Join our interdisciplinary team at #TUDelft in 2023!
#ScienceJobs #job
Delft #Netherlands #PhDStudent
https://jobrxiv.org/job/tu-delft-27778-phd-position-acoustofluidic-single-molecule-nanosystems/?feed_id=41694 -
"Boosting the toolbox for live imaging of translation"
The authors developed a new set of #Suntag tools (differnt green fluorescent proteins, different promoter for scFv) - optimizing them and identifying possible artefacts. these are primarily for #Drosophila but I guess could be adapted to other organisms.
They also developed a new system called ALFA_array which they test in fly and mammalian cells.
#mRNA #translation #microscopy #LiveCellImaging #SingleMolecule
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#bps2023 Come see @Tingting_Wu_ tell a beautiful story on “Mapping Inhomogeneous Network Structures within Biomolecular Condensate using #SingleMolecule Imaging and Tracking of Fluorogenic Probes,” tomorrow at 9:15 AM in Room 6C, a collaboration with #PappulabWashU
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We'll also be presenting “A Multi-View Reflector Microscope Visualizes #SixDimensional #SingleMolecule Translational And Rotational Dynamics With Isotropic Nanoscale Resolution!” Poster 1351-Pos / B480 #bps2023 https://doi.org/10.1016/j.bpj.2022.11.1587
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#bps2023 Come hear Brian talk about “#SingleMolecule Orientation-Localization Microscopy Resolves How Amyloidophilic Dye Orientations are Correlated with Amyloid Fiber Growth and Disruption,” Poster 1339-Pos / B468 tomorrow at 1:45 PM! https://doi.org/10.1016/j.bpj.2022.11.1575
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#bps2023 Come visit Weiyan tomorrow as she presents “#SingleMolecule Orientation-Localization Microscopy Resolves the Nanoscale Organization of Self-Assembled Peptides,” Poster 627-Pos / B448 at 1:45 PM in Exhibit Hall BC https://doi.org/10.1016/j.bpj.2022.11.863
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This just published from Jeff Chao's lab
@MolecularCell"Single-molecule imaging reveals translation-dependent destabilization of mRNAs"
#mRNA #mRNADegradation #miRNA #translation #imaging #SingleMolecule #NMD
https://www.cell.com/molecular-cell/fulltext/S1097-2765(23)00033-3
When the bioRxiv came out (along with 2 others on similar topic), I wrote about it in my blog:
There are several additions in the print version, e.g. adding miR-21. but its just 1st impression. Have to read it.
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Happy to share new collaborative work on #singlemolecule imaging of biomolecular #condensates. They're less uniform than they seem! With #PappulabWashU @TingtingWu #MattKingLab #MinaFaragSci #WashUengineers
"Single fluorogen imaging reveals spatial inhomogeneities within biomolecular condensates"
https://doi.org/10.1101/2023.01.26.525727