#smfish — Public Fediverse posts
Live and recent posts from across the Fediverse tagged #smfish, aggregated by home.social.
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🎯🐠 I've been away for a while. But here:
I wrote a blog post on "Imaging mRNA modifications in situ"Featuring: m6AISH-PLA, ARPLA (on #glycoRNA) & DART-FISH.
#smFISH #m6A #RNA #mRNA #RNAmodifications #microscopy #imaging #singlemolecule
https://greenfluorescentblog.wordpress.com/2023/10/31/imaging-mrna-modifications-in-situ/
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🎯🐠 I've been away for a while. But here:
I wrote a blog post on "Imaging mRNA modifications in situ"Featuring: m6AISH-PLA, ARPLA (on #glycoRNA) & DART-FISH.
#smFISH #m6A #RNA #mRNA #RNAmodifications #microscopy #imaging #singlemolecule
https://greenfluorescentblog.wordpress.com/2023/10/31/imaging-mrna-modifications-in-situ/
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🎯🐠 I've been away for a while. But here:
I wrote a blog post on "Imaging mRNA modifications in situ"Featuring: m6AISH-PLA, ARPLA (on #glycoRNA) & DART-FISH.
#smFISH #m6A #RNA #mRNA #RNAmodifications #microscopy #imaging #singlemolecule
https://greenfluorescentblog.wordpress.com/2023/10/31/imaging-mrna-modifications-in-situ/
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🎯🐠 I've been away for a while. But here:
I wrote a blog post on "Imaging mRNA modifications in situ"Featuring: m6AISH-PLA, ARPLA (on #glycoRNA) & DART-FISH.
#smFISH #m6A #RNA #mRNA #RNAmodifications #microscopy #imaging #singlemolecule
https://greenfluorescentblog.wordpress.com/2023/10/31/imaging-mrna-modifications-in-situ/
-
🎯🐠 I've been away for a while. But here:
I wrote a blog post on "Imaging mRNA modifications in situ"Featuring: m6AISH-PLA, ARPLA (on #glycoRNA) & DART-FISH.
#smFISH #m6A #RNA #mRNA #RNAmodifications #microscopy #imaging #singlemolecule
https://greenfluorescentblog.wordpress.com/2023/10/31/imaging-mrna-modifications-in-situ/
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Wow! 💥 🔥 🔥 🔥
This is so cool!
In this paper,
"Spatially resolved single-cell translatomics at molecular resolution", they used a tri-probe FISH system combined with in situ amplification and in situ sequencing to detect multiplex translating mRNAs at sub-cellular resolution in cell culture (981 genes) and mouse brain tissue slices (5413 genes!). -
Wow! 💥 🔥 🔥 🔥
This is so cool!
In this paper,
"Spatially resolved single-cell translatomics at molecular resolution", they used a tri-probe FISH system combined with in situ amplification and in situ sequencing to detect multiplex translating mRNAs at sub-cellular resolution in cell culture (981 genes) and mouse brain tissue slices (5413 genes!). -
Wow! 💥 🔥 🔥 🔥
This is so cool!
In this paper,
"Spatially resolved single-cell translatomics at molecular resolution", they used a tri-probe FISH system combined with in situ amplification and in situ sequencing to detect multiplex translating mRNAs at sub-cellular resolution in cell culture (981 genes) and mouse brain tissue slices (5413 genes!). -
Wow! 💥 🔥 🔥 🔥
This is so cool!
In this paper,
"Spatially resolved single-cell translatomics at molecular resolution", they used a tri-probe FISH system combined with in situ amplification and in situ sequencing to detect multiplex translating mRNAs at sub-cellular resolution in cell culture (981 genes) and mouse brain tissue slices (5413 genes!). -
Wow! 💥 🔥 🔥 🔥
This is so cool!
In this paper,
"Spatially resolved single-cell translatomics at molecular resolution", they used a tri-probe FISH system combined with in situ amplification and in situ sequencing to detect multiplex translating mRNAs at sub-cellular resolution in cell culture (981 genes) and mouse brain tissue slices (5413 genes!). -
Wow!
looks like super awesome method from Long Cai lab"High-resolution spatial multi-omics reveals cell-type specific nuclear compartments"
They did mRNA #seqFISH+ (1200 genes, 17K introns), DNA seqFISH+ (100K loci) and #immunofluorescence (up to 65 antibodies) all in the same experiment!
200+ rounds of #imaging! That's incredible.#mRNA #mRNALocalization #smFISH #DNA #3D_genome
#transcription #microscopy -
Wow!
looks like super awesome method from Long Cai lab"High-resolution spatial multi-omics reveals cell-type specific nuclear compartments"
They did mRNA #seqFISH+ (1200 genes, 17K introns), DNA seqFISH+ (100K loci) and #immunofluorescence (up to 65 antibodies) all in the same experiment!
200+ rounds of #imaging! That's incredible.#mRNA #mRNALocalization #smFISH #DNA #3D_genome
#transcription #microscopy -
Wow!
looks like super awesome method from Long Cai lab"High-resolution spatial multi-omics reveals cell-type specific nuclear compartments"
They did mRNA #seqFISH+ (1200 genes, 17K introns), DNA seqFISH+ (100K loci) and #immunofluorescence (up to 65 antibodies) all in the same experiment!
200+ rounds of #imaging! That's incredible.#mRNA #mRNALocalization #smFISH #DNA #3D_genome
#transcription #microscopy -
Wow!
looks like super awesome method from Long Cai lab"High-resolution spatial multi-omics reveals cell-type specific nuclear compartments"
They did mRNA #seqFISH+ (1200 genes, 17K introns), DNA seqFISH+ (100K loci) and #immunofluorescence (up to 65 antibodies) all in the same experiment!
200+ rounds of #imaging! That's incredible.#mRNA #mRNALocalization #smFISH #DNA #3D_genome
#transcription #microscopy -
Wow!
looks like super awesome method from Long Cai lab"High-resolution spatial multi-omics reveals cell-type specific nuclear compartments"
They did mRNA #seqFISH+ (1200 genes, 17K introns), DNA seqFISH+ (100K loci) and #immunofluorescence (up to 65 antibodies) all in the same experiment!
200+ rounds of #imaging! That's incredible.#mRNA #mRNALocalization #smFISH #DNA #3D_genome
#transcription #microscopy -
This week's #FluorescenceFriday user is Jasmin Camacho, a postdoctoral research associate in the Rohner Lab at the Stowers Institute for Medical Research! Jasmin appreciates HCR RNA-FISH's ability to visualize multiple genes in a single sample, especially since she works with precious bat samples that are hard to acquire. Check out some of her stunning single molecule FISH images of the small intestinal villi and crypts of a nectar bat here:
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"HT-smFISH: a cost-effective and flexible workflow for high-throughput single-molecule RNA imaging"
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"HT-smFISH: a cost-effective and flexible workflow for high-throughput single-molecule RNA imaging"
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"HT-smFISH: a cost-effective and flexible workflow for high-throughput single-molecule RNA imaging"
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"HT-smFISH: a cost-effective and flexible workflow for high-throughput single-molecule RNA imaging"
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I tried the original protocol a few years ago and didn't work well. inconsistent labeling (some good, some poor) and wasn't *that* cheap.
but maybe time to re-visit this protocol with this improved version:"Improved enzymatic labeling of fluorescent in situ hybridization probes applied to the visualization of retained introns in cells"
@biorxivpreprint -
I tried the original protocol a few years ago and didn't work well. inconsistent labeling (some good, some poor) and wasn't *that* cheap.
but maybe time to re-visit this protocol with this improved version:"Improved enzymatic labeling of fluorescent in situ hybridization probes applied to the visualization of retained introns in cells"
@biorxivpreprint -
I tried the original protocol a few years ago and didn't work well. inconsistent labeling (some good, some poor) and wasn't *that* cheap.
but maybe time to re-visit this protocol with this improved version:"Improved enzymatic labeling of fluorescent in situ hybridization probes applied to the visualization of retained introns in cells"
@biorxivpreprint -
I tried the original protocol a few years ago and didn't work well. inconsistent labeling (some good, some poor) and wasn't *that* cheap.
but maybe time to re-visit this protocol with this improved version:"Improved enzymatic labeling of fluorescent in situ hybridization probes applied to the visualization of retained introns in cells"
@biorxivpreprint -
CW: Trying RS-FISH plugin for FISH spot detection
RS-FISH is a new #FIJI plugin for single-molecule FISH analysis
https://www.nature.com/articles/s41592-022-01669-y
I spent an hour playing with it. Installation was very easy & instructions of use are clear. I got nice spot detection on a not-so-nice FISH image.
I need to learn more about this tool, particularly using with masks & batch processing - but seems very nice & easy to use.
@preibischs @successprocess
#mRNA #fluorescence #smFISH #SingleMolecule #microscopy #imaging -
CW: Trying RS-FISH plugin for FISH spot detection
RS-FISH is a new #FIJI plugin for single-molecule FISH analysis
https://www.nature.com/articles/s41592-022-01669-y
I spent an hour playing with it. Installation was very easy & instructions of use are clear. I got nice spot detection on a not-so-nice FISH image.
I need to learn more about this tool, particularly using with masks & batch processing - but seems very nice & easy to use.
@preibischs @successprocess
#mRNA #fluorescence #smFISH #SingleMolecule #microscopy #imaging -
CW: Trying RS-FISH plugin for FISH spot detection
RS-FISH is a new #FIJI plugin for single-molecule FISH analysis
https://www.nature.com/articles/s41592-022-01669-y
I spent an hour playing with it. Installation was very easy & instructions of use are clear. I got nice spot detection on a not-so-nice FISH image.
I need to learn more about this tool, particularly using with masks & batch processing - but seems very nice & easy to use.
@preibischs @successprocess
#mRNA #fluorescence #smFISH #SingleMolecule #microscopy #imaging -
CW: Trying RS-FISH plugin for FISH spot detection
RS-FISH is a new #FIJI plugin for single-molecule FISH analysis
https://www.nature.com/articles/s41592-022-01669-y
I spent an hour playing with it. Installation was very easy & instructions of use are clear. I got nice spot detection on a not-so-nice FISH image.
I need to learn more about this tool, particularly using with masks & batch processing - but seems very nice & easy to use.
@preibischs @successprocess
#mRNA #fluorescence #smFISH #SingleMolecule #microscopy #imaging -
"RS-FISH: precise, interactive, fast, and scalable FISH spot detection"
New, user-friendly, FISH spot detection software; runs as a #Fiji plugin.
From Stephen Preibisch lab with @successprocess and Kyle Harrington
Out in @naturemethods
#mRNA #fluorescence #smFISH #SingleMolecule #microscopy #imaging -
"RS-FISH: precise, interactive, fast, and scalable FISH spot detection"
New, user-friendly, FISH spot detection software; runs as a #Fiji plugin.
From Stephen Preibisch lab with @successprocess and Kyle Harrington
Out in @naturemethods
#mRNA #fluorescence #smFISH #SingleMolecule #microscopy #imaging -
"RS-FISH: precise, interactive, fast, and scalable FISH spot detection"
New, user-friendly, FISH spot detection software; runs as a #Fiji plugin.
From Stephen Preibisch lab with @successprocess and Kyle Harrington
Out in @naturemethods
#mRNA #fluorescence #smFISH #SingleMolecule #microscopy #imaging -
"RS-FISH: precise, interactive, fast, and scalable FISH spot detection"
New, user-friendly, FISH spot detection software; runs as a #Fiji plugin.
From Stephen Preibisch lab with @successprocess and Kyle Harrington
Out in @naturemethods
#mRNA #fluorescence #smFISH #SingleMolecule #microscopy #imaging