#smfish — Public Fediverse posts
Live and recent posts from across the Fediverse tagged #smfish, aggregated by home.social.
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🎯🐠 I've been away for a while. But here:
I wrote a blog post on "Imaging mRNA modifications in situ"Featuring: m6AISH-PLA, ARPLA (on #glycoRNA) & DART-FISH.
#smFISH #m6A #RNA #mRNA #RNAmodifications #microscopy #imaging #singlemolecule
https://greenfluorescentblog.wordpress.com/2023/10/31/imaging-mrna-modifications-in-situ/
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Wow!
looks like super awesome method from Long Cai lab"High-resolution spatial multi-omics reveals cell-type specific nuclear compartments"
They did mRNA #seqFISH+ (1200 genes, 17K introns), DNA seqFISH+ (100K loci) and #immunofluorescence (up to 65 antibodies) all in the same experiment!
200+ rounds of #imaging! That's incredible.#mRNA #mRNALocalization #smFISH #DNA #3D_genome
#transcription #microscopy