#mrnalocalization — Public Fediverse posts
Live and recent posts from across the Fediverse tagged #mrnalocalization, aggregated by home.social.
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🔥 📣 This is probably bad timing, with all the political chaos in Israel, but we're looking for a PhD student to continue a grad student's project on mRNA transfer through tunneling nanotubes.
Start date ~Jan 2024.
We are looking for someone interested in deciphering the molecular mechanism of mRNA transfer, by performing a CRISPR/siRNA/drug screen, and using molecular biology & biochemistry tools.
(more next post >>) -
🔥 📣 This is probably bad timing, with all the political chaos in Israel, but we're looking for a PhD student to continue a grad student's project on mRNA transfer through tunneling nanotubes.
Start date ~Jan 2024.
We are looking for someone interested in deciphering the molecular mechanism of mRNA transfer, by performing a CRISPR/siRNA/drug screen, and using molecular biology & biochemistry tools.
(more next post >>) -
🔥 📣 This is probably bad timing, with all the political chaos in Israel, but we're looking for a PhD student to continue a grad student's project on mRNA transfer through tunneling nanotubes.
Start date ~Jan 2024.
We are looking for someone interested in deciphering the molecular mechanism of mRNA transfer, by performing a CRISPR/siRNA/drug screen, and using molecular biology & biochemistry tools.
(more next post >>) -
🔥 📣 This is probably bad timing, with all the political chaos in Israel, but we're looking for a PhD student to continue a grad student's project on mRNA transfer through tunneling nanotubes.
Start date ~Jan 2024.
We are looking for someone interested in deciphering the molecular mechanism of mRNA transfer, by performing a CRISPR/siRNA/drug screen, and using molecular biology & biochemistry tools.
(more next post >>) -
🔥 📣 This is probably bad timing, with all the political chaos in Israel, but we're looking for a PhD student to continue a grad student's project on mRNA transfer through tunneling nanotubes.
Start date ~Jan 2024.
We are looking for someone interested in deciphering the molecular mechanism of mRNA transfer, by performing a CRISPR/siRNA/drug screen, and using molecular biology & biochemistry tools.
(more next post >>) -
Wow! 💥 🔥 🔥 🔥
This is so cool!
In this paper,
"Spatially resolved single-cell translatomics at molecular resolution", they used a tri-probe FISH system combined with in situ amplification and in situ sequencing to detect multiplex translating mRNAs at sub-cellular resolution in cell culture (981 genes) and mouse brain tissue slices (5413 genes!). -
Wow! 💥 🔥 🔥 🔥
This is so cool!
In this paper,
"Spatially resolved single-cell translatomics at molecular resolution", they used a tri-probe FISH system combined with in situ amplification and in situ sequencing to detect multiplex translating mRNAs at sub-cellular resolution in cell culture (981 genes) and mouse brain tissue slices (5413 genes!). -
Wow! 💥 🔥 🔥 🔥
This is so cool!
In this paper,
"Spatially resolved single-cell translatomics at molecular resolution", they used a tri-probe FISH system combined with in situ amplification and in situ sequencing to detect multiplex translating mRNAs at sub-cellular resolution in cell culture (981 genes) and mouse brain tissue slices (5413 genes!). -
Wow! 💥 🔥 🔥 🔥
This is so cool!
In this paper,
"Spatially resolved single-cell translatomics at molecular resolution", they used a tri-probe FISH system combined with in situ amplification and in situ sequencing to detect multiplex translating mRNAs at sub-cellular resolution in cell culture (981 genes) and mouse brain tissue slices (5413 genes!). -
Wow! 💥 🔥 🔥 🔥
This is so cool!
In this paper,
"Spatially resolved single-cell translatomics at molecular resolution", they used a tri-probe FISH system combined with in situ amplification and in situ sequencing to detect multiplex translating mRNAs at sub-cellular resolution in cell culture (981 genes) and mouse brain tissue slices (5413 genes!). -
Interesting paper from Tatjana Trcek lab.
She & her team looked at fly #GermGranules nucleation and, well... the title says it all:"Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila "
#mRNA #mRNALocalization #Drosophila #LLPS
It is also her 1st paper as #NewPI so congrats are in order.
https://www.sciencedirect.com/science/article/pii/S2211124723007349
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Interesting paper from Tatjana Trcek lab.
She & her team looked at fly #GermGranules nucleation and, well... the title says it all:"Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila "
#mRNA #mRNALocalization #Drosophila #LLPS
It is also her 1st paper as #NewPI so congrats are in order.
https://www.sciencedirect.com/science/article/pii/S2211124723007349
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Interesting paper from Tatjana Trcek lab.
She & her team looked at fly #GermGranules nucleation and, well... the title says it all:"Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila "
#mRNA #mRNALocalization #Drosophila #LLPS
It is also her 1st paper as #NewPI so congrats are in order.
https://www.sciencedirect.com/science/article/pii/S2211124723007349
-
Interesting paper from Tatjana Trcek lab.
She & her team looked at fly #GermGranules nucleation and, well... the title says it all:"Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila "
#mRNA #mRNALocalization #Drosophila #LLPS
It is also her 1st paper as #NewPI so congrats are in order.
https://www.sciencedirect.com/science/article/pii/S2211124723007349
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Wow!
looks like super awesome method from Long Cai lab"High-resolution spatial multi-omics reveals cell-type specific nuclear compartments"
They did mRNA #seqFISH+ (1200 genes, 17K introns), DNA seqFISH+ (100K loci) and #immunofluorescence (up to 65 antibodies) all in the same experiment!
200+ rounds of #imaging! That's incredible.#mRNA #mRNALocalization #smFISH #DNA #3D_genome
#transcription #microscopy -
Wow!
looks like super awesome method from Long Cai lab"High-resolution spatial multi-omics reveals cell-type specific nuclear compartments"
They did mRNA #seqFISH+ (1200 genes, 17K introns), DNA seqFISH+ (100K loci) and #immunofluorescence (up to 65 antibodies) all in the same experiment!
200+ rounds of #imaging! That's incredible.#mRNA #mRNALocalization #smFISH #DNA #3D_genome
#transcription #microscopy -
Wow!
looks like super awesome method from Long Cai lab"High-resolution spatial multi-omics reveals cell-type specific nuclear compartments"
They did mRNA #seqFISH+ (1200 genes, 17K introns), DNA seqFISH+ (100K loci) and #immunofluorescence (up to 65 antibodies) all in the same experiment!
200+ rounds of #imaging! That's incredible.#mRNA #mRNALocalization #smFISH #DNA #3D_genome
#transcription #microscopy -
Wow!
looks like super awesome method from Long Cai lab"High-resolution spatial multi-omics reveals cell-type specific nuclear compartments"
They did mRNA #seqFISH+ (1200 genes, 17K introns), DNA seqFISH+ (100K loci) and #immunofluorescence (up to 65 antibodies) all in the same experiment!
200+ rounds of #imaging! That's incredible.#mRNA #mRNALocalization #smFISH #DNA #3D_genome
#transcription #microscopy -
Wow!
looks like super awesome method from Long Cai lab"High-resolution spatial multi-omics reveals cell-type specific nuclear compartments"
They did mRNA #seqFISH+ (1200 genes, 17K introns), DNA seqFISH+ (100K loci) and #immunofluorescence (up to 65 antibodies) all in the same experiment!
200+ rounds of #imaging! That's incredible.#mRNA #mRNALocalization #smFISH #DNA #3D_genome
#transcription #microscopy -
New pre-print from Voula Mili lab.
she suggests that mRNA localization and translation rate determine the localization of a protein which is found both in nucleus and cytoplasm.
I'll need to find time to dive it and read in detail. -
Wow, from the abstract this paper from Christine Mayr lab looks super interesting!
"mRNA interactions with disordered regions control protein activity"
#mRNA #translation #mRNALocalization #ProteinStructure #RNAbindinghttps://www.biorxiv.org/content/10.1101/2023.02.18.529068v1?med=mas
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"HT-smFISH: a cost-effective and flexible workflow for high-throughput single-molecule RNA imaging"
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"HT-smFISH: a cost-effective and flexible workflow for high-throughput single-molecule RNA imaging"
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"HT-smFISH: a cost-effective and flexible workflow for high-throughput single-molecule RNA imaging"
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"HT-smFISH: a cost-effective and flexible workflow for high-throughput single-molecule RNA imaging"
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I tried the original protocol a few years ago and didn't work well. inconsistent labeling (some good, some poor) and wasn't *that* cheap.
but maybe time to re-visit this protocol with this improved version:"Improved enzymatic labeling of fluorescent in situ hybridization probes applied to the visualization of retained introns in cells"
@biorxivpreprint -
I tried the original protocol a few years ago and didn't work well. inconsistent labeling (some good, some poor) and wasn't *that* cheap.
but maybe time to re-visit this protocol with this improved version:"Improved enzymatic labeling of fluorescent in situ hybridization probes applied to the visualization of retained introns in cells"
@biorxivpreprint -
I tried the original protocol a few years ago and didn't work well. inconsistent labeling (some good, some poor) and wasn't *that* cheap.
but maybe time to re-visit this protocol with this improved version:"Improved enzymatic labeling of fluorescent in situ hybridization probes applied to the visualization of retained introns in cells"
@biorxivpreprint -
I tried the original protocol a few years ago and didn't work well. inconsistent labeling (some good, some poor) and wasn't *that* cheap.
but maybe time to re-visit this protocol with this improved version:"Improved enzymatic labeling of fluorescent in situ hybridization probes applied to the visualization of retained introns in cells"
@biorxivpreprint -
So, after lurking here for a couple of days, I realized that hashtags are important here. So here are a few of my interests:
#RNA
#mRNA
#mRNALocalization
#ribosome #P_bodies #StressGranules
#Transcription #Translation #mRNADegradation
#CellBiology #MolecularBiology
#TunnelingNanotubes
#EndoplasmicReticulum #mitochondria #Peroxisome
#Microscopy #fluorescent #FluorescentProteins
#PeerReview #StaffScientist
#Reproducibility
#LabLife -
So, after lurking here for a couple of days, I realized that hashtags are important here. So here are a few of my interests:
#RNA
#mRNA
#mRNALocalization
#ribosome #P_bodies #StressGranules
#Transcription #Translation #mRNADegradation
#CellBiology #MolecularBiology
#TunnelingNanotubes
#EndoplasmicReticulum #mitochondria #Peroxisome
#Microscopy #fluorescent #FluorescentProteins
#PeerReview #StaffScientist
#Reproducibility
#LabLife -
So, after lurking here for a couple of days, I realized that hashtags are important here. So here are a few of my interests:
#RNA
#mRNA
#mRNALocalization
#ribosome #P_bodies #StressGranules
#Transcription #Translation #mRNADegradation
#CellBiology #MolecularBiology
#TunnelingNanotubes
#EndoplasmicReticulum #mitochondria #Peroxisome
#Microscopy #fluorescent #FluorescentProteins
#PeerReview #StaffScientist
#Reproducibility
#LabLife -
So, after lurking here for a couple of days, I realized that hashtags are important here. So here are a few of my interests:
#RNA
#mRNA
#mRNALocalization
#ribosome #P_bodies #StressGranules
#Transcription #Translation #mRNADegradation
#CellBiology #MolecularBiology
#TunnelingNanotubes
#EndoplasmicReticulum #mitochondria #Peroxisome
#Microscopy #fluorescent #FluorescentProteins
#PeerReview #StaffScientist
#Reproducibility
#LabLife -
So, after lurking here for a couple of days, I realized that hashtags are important here. So here are a few of my interests:
#RNA
#mRNA
#mRNALocalization
#ribosome #P_bodies #StressGranules
#Transcription #Translation #mRNADegradation
#CellBiology #MolecularBiology
#TunnelingNanotubes
#EndoplasmicReticulum #mitochondria #Peroxisome
#Microscopy #fluorescent #FluorescentProteins
#PeerReview #StaffScientist
#Reproducibility
#LabLife