#mrnalocalization — Public Fediverse posts

Wow! 💥 🔥 🔥 🔥
This is so cool!
In this paper,
"Spatially resolved single-cell translatomics at molecular resolution", they used a tri-probe FISH system combined with in situ amplification and in situ sequencing to detect multiplex translating mRNAs at sub-cellular resolution in cell culture (981 genes) and mouse brain tissue slices (5413 genes!).

#mRNALocalization #mRNA #translation #smFISH #FISHseq

https://www.science.org/doi/10.1126/science.add3067

Interesting paper from Tatjana Trcek lab.
She & her team looked at fly #GermGranules nucleation and, well... the title says it all:

"Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila "

#mRNA #mRNALocalization #Drosophila #LLPS

It is also her 1st paper as #NewPI so congrats are in order.

https://www.sciencedirect.com/science/article/pii/S2211124723007349

Wow!
looks like super awesome method from Long Cai lab

"High-resolution spatial multi-omics reveals cell-type specific nuclear compartments"

They did mRNA #seqFISH+ (1200 genes, 17K introns), DNA seqFISH+ (100K loci) and #immunofluorescence (up to 65 antibodies) all in the same experiment!
200+ rounds of #imaging! That's incredible.

#mRNA #mRNALocalization #smFISH #DNA #3D_genome
#transcription #microscopy

https://www.biorxiv.org/content/10.1101/2023.05.07.539762v1

"HT-smFISH: a cost-effective and flexible workflow for high-throughput single-molecule RNA imaging"

#mRNA #mRNALocalization #smFISH

https://www.nature.com/articles/s41596-022-00750-2

I tried the original protocol a few years ago and didn't work well. inconsistent labeling (some good, some poor) and wasn't *that* cheap.
but maybe time to re-visit this protocol with this improved version:

"Improved enzymatic labeling of fluorescent in situ hybridization probes applied to the visualization of retained introns in cells"
@biorxivpreprint

#mRNA #mRNALocalization #smFISH

https://www.biorxiv.org/content/10.1101/2023.01.10.523484v1

So, after lurking here for a couple of days, I realized that hashtags are important here. So here are a few of my interests:
#RNA
#mRNA
#mRNALocalization
#ribosome #P_bodies #StressGranules
#Transcription #Translation #mRNADegradation
#CellBiology #MolecularBiology
#TunnelingNanotubes
#EndoplasmicReticulum #mitochondria #Peroxisome
#Microscopy #fluorescent #FluorescentProteins
#PeerReview #StaffScientist
#Reproducibility
#LabLife