#immunofluorescence — Public Fediverse posts

#Methode
Fluoreszenz-Marathonläufer vs. Sprinter: Forschende der @unigoettingen
nutzen #FluoreszenzlebensdauerMikroskopie & HaloTag-Nanobodies, um Zielproteine zu detektieren und trotz spektraler Überlappung exakt auseinander zu halten.

Mehr im Artikel von Andrea Pitzschke: https://www.laborjournal.de/editorials/3450.php

#Laborjournal #LifeSci #FLIM #Nanobodies #HaloTag #Mikroskopie #Zellbiologie #MultiplexImaging #Immunofluorescence

#Methode
Fluoreszenz-Marathonläufer vs. Sprinter: Forschende der @unigoettingen
nutzen #FluoreszenzlebensdauerMikroskopie & HaloTag-Nanobodies, um Zielproteine zu detektieren und trotz spektraler Überlappung exakt auseinander zu halten.

Mehr im Artikel von Andrea Pitzschke: https://www.laborjournal.de/editorials/3450.php

#Laborjournal #LifeSci #FLIM #Nanobodies #HaloTag #Mikroskopie #Zellbiologie #MultiplexImaging #Immunofluorescence

The IBEX Knowledge-Base is setting a new standard for reproducibility in #immunofluorescence imaging: open, community-driven, and with ‘exceptional’ evidence of its utility.

It’s now up to the community to contribute to its growth.

🔗 https://elifesciences.org/reviewed-preprints/105737?utm_source=mastodon&utm_medium=social&utm_campaign=organic

The IBEX Knowledge-Base is setting a new standard for reproducibility in #immunofluorescence imaging: open, community-driven, and with ‘exceptional’ evidence of its utility.

It’s now up to the community to contribute to its growth.

🔗 https://elifesciences.org/reviewed-preprints/105737?utm_source=mastodon&utm_medium=social&utm_campaign=organic

This is an #immunofluorescence image of a section of mouse testis spermatocytes, Sertoli cells, and GPX4. Researchers investigated the impact of mitochondrial defects on sperm cells. #Mitochondria #12DaysOfeLife https://elifesciences.org/articles/84710?utm_source=mastodon&utm_medium=social&utm_campaign=organic_12Days

This is an #immunofluorescence image of a section of mouse testis spermatocytes, Sertoli cells, and GPX4. Researchers investigated the impact of mitochondrial defects on sperm cells. #Mitochondria #12DaysOfeLife https://elifesciences.org/articles/84710?utm_source=mastodon&utm_medium=social&utm_campaign=organic_12Days

@domhenri Wow, those numbers are depressing.

Figure 3 should scare everyone who uses antibodies (and hopefully spur them to sanity-check their stainings with additional techniques).

https://elifesciences.org/reviewed-preprints/91645v1#fig3

#bioscience #immunostaining #immunofluorescence

Wow!
looks like super awesome method from Long Cai lab

"High-resolution spatial multi-omics reveals cell-type specific nuclear compartments"

They did mRNA #seqFISH+ (1200 genes, 17K introns), DNA seqFISH+ (100K loci) and #immunofluorescence (up to 65 antibodies) all in the same experiment!
200+ rounds of #imaging! That's incredible.

#mRNA #mRNALocalization #smFISH #DNA #3D_genome
#transcription #microscopy

https://www.biorxiv.org/content/10.1101/2023.05.07.539762v1

Fluorescein isothiocyanate (FITC) is an organic fluorescent dye and probably one of the most commonly used in #immunofluorescence and flow cytometry.
Learn more about Fluorescent Dyes: https://fcld.ly/b5zd1oh

🖼️ Drosophila embryo development, Green: FITC, Red: TRITC.

#fluorescencefriday #fluorescence #dyes #microscopy #science #sciencemastodon