#m6a — Public Fediverse posts

Post-transcriptional #GeneRegulation through #m6A deposition is dysregulated in many diseases. In this #PLOSBiology Unsolved Mystery, Samie Jaffrey &co investigate why the m6A writer complex requires multiple subunits and what roles its individual proteins might have in m6A regulation.
https://plos.io/3VjfjWI

beloved #Mettl3 is involved in DNA repair by placing #m6A in #DNA
#modifications
https://elifesciences.org/articles/101626

An #m6A-independent function of #YTHDC1: its expression delays cellular #senescence and pulmonary #fibrosis by interacting with #TopBP1 to activate ATR
https://www.embopress.org/doi/full/10.1038/s44318-023-00003-2

Can you imagine calling #m6A modifications from raw #RNA #nanopore reads just with #basecalling?

Let me introduce you to #m6ABasecaller

https://doi.org/10.1101/2023.11.13.566801

Can you imagine calling #m6A modifications from raw #RNA #nanopore reads just with #basecalling?

Let me introduce you to #m6ABasecaller

https://doi.org/10.1101/2023.11.13.566801

Can you imagine calling #m6A modifications from raw #RNA #nanopore reads just with #basecalling?

Let me introduce you to #m6ABasecaller

https://doi.org/10.1101/2023.11.13.566801

Can you imagine calling #m6A modifications from raw #RNA #nanopore reads just with #basecalling?

Let me introduce you to #m6ABasecaller

https://doi.org/10.1101/2023.11.13.566801

Can you imagine calling #m6A modifications from raw #RNA #nanopore reads just with #basecalling?

Let me introduce you to #m6ABasecaller

https://doi.org/10.1101/2023.11.13.566801

🎯🐠 I've been away for a while. But here:
I wrote a blog post on "Imaging mRNA modifications in situ"

Featuring: m6AISH-PLA, ARPLA (on #glycoRNA) & DART-FISH.

#smFISH #m6A #RNA #mRNA #RNAmodifications #microscopy #imaging #singlemolecule

https://greenfluorescentblog.wordpress.com/2023/10/31/imaging-mrna-modifications-in-situ/

In our issue 18:
Reviews on ubiquitin-targeted bacterial effectors, and on satellite RNAs

Highlights on #ribonucleotide salvage for #mitochondrial #transcription, and plant defense against #m6A-RNA-containing viruses

Further articles on #intraflagellar transport, #replication initiation within #chromatin, #integrin-dependent cancer cell invasion…

https://www.embopress.org/toc/14602075/2023/42/18

Nice!
Another "uncharacterized" gene (YGL036W) in yeast is now characterized - as encoding a protein (re-named Vir1) that is part of the mRNA m6A methyltransferase complex required during meiosis.

The deletion mutant is defective in meiosis and has zero m6A, just like ime4 deletion.

"Vir1p, the Yeast Homolog of Virilizer, is Required for mRNA m6A Methylation and Meiosis"

#mRNA #m6A #yeast #meiosis

@biorxivpreprint

https://www.biorxiv.org/content/10.1101/2023.02.07.527493v1

Very interesting paper from Schraga Schwartz (in our department) and his student Anna Uzonyi (who won best talk at the latest Israeli #RNA society meeting for presenting this work).
#m6A is an #RNAmodification regulating #mRNADegradation.

They show that m6A is deposited on all #mRNA molecules by default, but is excluded in the vicinity of splice junctions by EJC.
So m6A or lack of it is a memory of nuclear processing.

@MolecularCell

https://www.cell.com/molecular-cell/fulltext/S1097-2765(22)01205-9

How does N6-methylA (m6A) #RNAmodification influence lncRNAs? @DrPorman @ChromaTownie &co show that the #lncRNA HOTAIR represses or activates genes, depending on a single #m6A modification, to affect #BreastCancer cell biology @rnabioco #PLOSBiology https://plos.io/3VzO5ZT

How does N6-methylA (m6A) #RNAmodification influence lncRNAs? @DrPorman @ChromaTownie &co show that the #lncRNA HOTAIR represses or activates genes, depending on a single #m6A modification, to affect #BreastCancer cell biology @rnabioco #PLOSBiology https://plos.io/3VzO5ZT

How does N6-methylA (m6A) #RNAmodification influence lncRNAs? @DrPorman @ChromaTownie &co show that the #lncRNA HOTAIR represses or activates genes, depending on a single #m6A modification, to affect #BreastCancer cell biology @rnabioco #PLOSBiology https://plos.io/3VzO5ZT