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#vem — Public Fediverse posts

Live and recent posts from across the Fediverse tagged #vem, aggregated by home.social.

  1. @innuendo @jernej__s @ZachWeinersmith

    Our eFIB-SEM (as per Xu et al. 2017 elifesciences.org/articles/259 ) runs 24/7 for up to 8 weeks, when it runs out of source. And it's essential that it does so, uninterrupted, since it's imaging at nanometre resolution, milling 2 nm slices at a time with an ion beam and then repeatedly imaging the block face. The process is unsurprisingly very sensitive; was hard enough to overcome the reheat cycle of the instrument itself (every 60 hours or so) by detecting it, de-engaging the beams and then seamlessly re-engaging with the block face.

    A brief video of the kind of data we acquire:
    youtu.be/p4MH4-I-P5I

    The MS Windows computer deciding to reboot on its own is unacceptable.

    #ElectronMicroscopy #Zeiss #FIBSEM #VolumeEM #vEM #Drosophila

  2. @innuendo @jernej__s @ZachWeinersmith

    Our eFIB-SEM (as per Xu et al. 2017 elifesciences.org/articles/259 ) runs 24/7 for up to 8 weeks, when it runs out of source. And it's essential that it does so, uninterrupted, since it's imaging at nanometre resolution, milling 2 nm slices at a time with an ion beam and then repeatedly imaging the block face. The process is unsurprisingly very sensitive; was hard enough to overcome the reheat cycle of the instrument itself (every 60 hours or so) by detecting it, de-engaging the beams and then seamlessly re-engaging with the block face.

    A brief video of the kind of data we acquire:
    youtu.be/p4MH4-I-P5I

    The MS Windows computer deciding to reboot on its own is unacceptable.

    #ElectronMicroscopy #Zeiss #FIBSEM #VolumeEM #vEM #Drosophila

  3. @innuendo @jernej__s @ZachWeinersmith

    Our eFIB-SEM (as per Xu et al. 2017 elifesciences.org/articles/259 ) runs 24/7 for up to 8 weeks, when it runs out of source. And it's essential that it does so, uninterrupted, since it's imaging at nanometre resolution, milling 2 nm slices at a time with an ion beam and then repeatedly imaging the block face. The process is unsurprisingly very sensitive; was hard enough to overcome the reheat cycle of the instrument itself (every 60 hours or so) by detecting it, de-engaging the beams and then seamlessly re-engaging with the block face.

    A brief video of the kind of data we acquire:
    youtu.be/p4MH4-I-P5I

    The MS Windows computer deciding to reboot on its own is unacceptable.

    #ElectronMicroscopy #Zeiss #FIBSEM #VolumeEM #vEM #Drosophila

  4. @innuendo @jernej__s @ZachWeinersmith

    Our eFIB-SEM (as per Xu et al. 2017 elifesciences.org/articles/259 ) runs 24/7 for up to 8 weeks, when it runs out of source. And it's essential that it does so, uninterrupted, since it's imaging at nanometre resolution, milling 2 nm slices at a time with an ion beam and then repeatedly imaging the block face. The process is unsurprisingly very sensitive; was hard enough to overcome the reheat cycle of the instrument itself (every 60 hours or so) by detecting it, de-engaging the beams and then seamlessly re-engaging with the block face.

    A brief video of the kind of data we acquire:
    youtu.be/p4MH4-I-P5I

    The MS Windows computer deciding to reboot on its own is unacceptable.

    #ElectronMicroscopy #Zeiss #FIBSEM #VolumeEM #vEM #Drosophila

  5. @innuendo @jernej__s @ZachWeinersmith

    Our eFIB-SEM (as per Xu et al. 2017 elifesciences.org/articles/259 ) runs 24/7 for up to 8 weeks, when it runs out of source. And it's essential that it does so, uninterrupted, since it's imaging at nanometre resolution, milling 2 nm slices at a time with an ion beam and then repeatedly imaging the block face. The process is unsurprisingly very sensitive; was hard enough to overcome the reheat cycle of the instrument itself (every 60 hours or so) by detecting it, de-engaging the beams and then seamlessly re-engaging with the block face.

    A brief video of the kind of data we acquire:
    youtu.be/p4MH4-I-P5I

    The MS Windows computer deciding to reboot on its own is unacceptable.

    #ElectronMicroscopy #Zeiss #FIBSEM #VolumeEM #vEM #Drosophila

  6. Nuno da Costa at the Allen Institute for Brain Research is hiring:

    "We are seeking an exceptional individual to develop innovative sectioning techniques for a large scale Electron Microscopy pipeline."

    alleninstitute.hrmdirect.com/e

    #PhDJobs #neuroscience #vEM #ElectronMicroscopy

  7. Nuno da Costa at the Allen Institute for Brain Research is hiring:

    "We are seeking an exceptional individual to develop innovative sectioning techniques for a large scale Electron Microscopy pipeline."

    alleninstitute.hrmdirect.com/e

    #PhDJobs #neuroscience #vEM #ElectronMicroscopy

  8. Nuno da Costa at the Allen Institute for Brain Research is hiring:

    "We are seeking an exceptional individual to develop innovative sectioning techniques for a large scale Electron Microscopy pipeline."

    alleninstitute.hrmdirect.com/e

    #PhDJobs #neuroscience #vEM #ElectronMicroscopy

  9. Nuno da Costa at the Allen Institute for Brain Research is hiring:

    "We are seeking an exceptional individual to develop innovative sectioning techniques for a large scale Electron Microscopy pipeline."

    alleninstitute.hrmdirect.com/e

    #PhDJobs #neuroscience #vEM #ElectronMicroscopy

  10. Nuno da Costa at the Allen Institute for Brain Research is hiring:

    "We are seeking an exceptional individual to develop innovative sectioning techniques for a large scale Electron Microscopy pipeline."

    alleninstitute.hrmdirect.com/e

    #PhDJobs #neuroscience #vEM #ElectronMicroscopy

  11. Das #Lichtsteuergerät SL 3930 wurde um 1988 im VEB Starkstrom-Anlagenbau #Leipzig #Halle #VEM als Ergänzung zur gleichnamigen #HiFi Kombination produziert. Das Gerät kombiniert ein Lauflicht-Steuergerät mit einer klassischen #Lichtorgel. Über die rückseitigen Schuko-Steckdosen können insgesamt 4 Strahler angeschlossen werden. Das #RFT SL 3930 folgte dem bereits in #Berlin produzierten #AKA #Lichteffektgerät.
    Die ausführlichen Beschreibungen habe ich unter 🏳️‍🌈 rk7.de/hifi 🏳️‍🌈 hinterlegt.😊

  12. Happy to be involved in UNICIL, our new Wellcome Trust Discovery project coordinated by @micromotility.bsky.social

    We will study ciliary dynamics across scales and organisms. A long-term #postdoc position will be available at Heidelberg University @uniheidelberg shortly.

    @uniofexeter press release:

    news.exeter.ac.uk/living-syste

    #biology #microscopy #vEM #cilia #platynereis

  13. Arrived at the Volume Electron Microscopy
    Gordon Research Conference

    grc.org/volume-electron-micros

    near Barcelona. I will speak about Volume EM and #connectomics in marine zoomplankton.

    #vEM #microscopy #connectomics

  14. "Comparative connectomics of Drosophila descending and ascending neurons", Tomke Stürner et al. 2025 (Greg Jefferis and Katharina Eichler's labs).
    nature.com/articles/s41586-025

    Compares between males and females.

    #neuroscience #Drosophila #connectomics #vEM #volumeEM

  15. The revised version of our #Platynereis #connectome paper is now out:

    elifesciences.org/reviewed-pre

    Cell-type-level annotation of the whole organisms, including synaptic and desmosomal connectomes. Can be explored with CATMAID here:
    catmaid-jekelylab.cos.uni-heid
    #larva #marine #neuroscience #vEM

  16. The revised version of our #Platynereis #connectome paper is now out:

    elifesciences.org/reviewed-pre

    Cell-type-level annotation of the whole organisms, including synaptic and desmosomal connectomes. Can be explored with CATMAID here:
    catmaid-jekelylab.cos.uni-heid
    #larva #marine #neuroscience #vEM

  17. The revised version of our #Platynereis #connectome paper is now out:

    elifesciences.org/reviewed-pre

    Cell-type-level annotation of the whole organisms, including synaptic and desmosomal connectomes. Can be explored with CATMAID here:
    catmaid-jekelylab.cos.uni-heid
    #larva #marine #neuroscience #vEM

  18. GridTape TEM with beam deflection to reduce stage movements and increase imaging throughput:

    "Fast imaging of millimeter-scale areas with beam deflection transmission electron microscopy", Zhao et al. 2024

    nature.com/articles/s41467-024

    #TEM #GridTapeTEM #vEM #VolumeEM #connectomics #neuroscience #microscopy #ElectronMicroscopy

  19. @nanographs

    Thanks for sharing this – will have to think about it. In our case, we built in-house the gridtape reel holders which amount to a custom stage with a mini-camera in it for identifying slots and controlling imaging. So we don't use the factory-supplied stage.

    biorxiv.org/content/10.1101/65

    #vEM #volumeEM #TEM

  20. True as always that the way to make software run faster is to make it do less operations. After all, CPUs can only execute a fixed number of operations per unit of time.

    Here, I tweaked code for serial section registration that drops execution time from 27 seconds to 100 milliseconds: a 270x speed up.

    All it had to do is to search for matching SIFT features in one image only within a predetermined radius centered on one SIFT feature in another image. Extremely effective for when e.g., the maximum translation is known.

    The matching code using a KDTree:
    github.com/acardona/scripts/bl

    The test script:
    github.com/acardona/scripts/bl

    #FijiSc #java #jython #volumeEM #vEM

  21. New paper from Ikeda et al. on the biogenesis of chitin bristles in the annelid #Platynereis with nice #vEM reconstructions and a chitin synthase knockout.
    Bristles are formed in a process of biological 3D printing. @biology
    #microscopy
    nature.com/articles/s41467-024

  22. From Moritz Helmstaedter on #RoboEM:

    "In today’s AI research, any project lasting longer than 6 months is considered slow, if not outdated and overrun by history. RoboEM, with a concept and an approach that seem quite plausible, still took 5 years from idea to the fully evaluated tool reported here. This was only possible with the patient support of the Max Planck Society, which encourages long-term projects, and the tenacity of the first author. Details made all the difference. And, as often, dead ends had to be avoided efficiently. We had to abandon, for example, the idea of using steering variability to indicate branch points — a nice analogy to road intersections in car steering, but too far-fetched to work well enough in brain tissue data. Sometimes giving up beloved ideas is as important as following through on others. M.H."

    nature.com/articles/s41592-024

    #connectomics #volumeEM #vEM #neuroscience #MaxPlanckSociety

  23. “Perception: How larvae feel the world around them” by Jimena Berni elifesciences.org/articles/967

    … an insight piece on Andreas Thum’s lab work on mapping the sensory organs of the #Drosophila larva with electron microscopy:

    “Morphology and ultrastructure of external sense organs of Drosophila larvae”
    Richter et al. 2024 elifesciences.org/articles/967

    #neuroscience #vEM #VolumeEM

  24. "DeepFocus: fast focus and astigmatism correction for electron microscopy", Schubert et al. 2024 (Kornfeld lab)

    nature.com/articles/s41467-024

    #neuroscience #vEM #ElectronMicroscopy

  25. A review of the current state of multimodal bioimaging:

    "Integrating cellular electron microscopy with multimodal data to explore biology across space and time", McCafferty et al. 2024 cell.com/cell/fulltext/S0092-8

    #vEM #cryoEM #bioimaging

  26. "SmartEM: machine-learning guided electron microscopy" by Meirovitch et al 2023 (Nir Shavit and Aravi Samuel labs)
    biorxiv.org/content/10.1101/20

    Turn up the imaging rate of your scanning electron microscope by only imaging at high resolution the areas of the image necessary to resolve ambiguities in the low-res image. Motivated by the need for high-throughput but inexpensive imaging for #connectomics.

    #neuroscience #vEM #ElectronMicroscopy

  27. Ich hab was für #StachelUndHerz gespendet, weil ich die Arbeit so wertvoll , wichtig und gut finde!

    betterplace.org/de/projects/12

    (Via Betterplace für bessere Sichtbarkeit - hab ich vorher via Mail abgeklärt) .

    #vem

  28. Ich hab was für #StachelUndHerz gespendet, weil ich die Arbeit so wertvoll , wichtig und gut finde!

    betterplace.org/de/projects/12

    (Via Betterplace für bessere Sichtbarkeit - hab ich vorher via Mail abgeklärt) .

    #vem

  29. Ich hab was für #StachelUndHerz gespendet, weil ich die Arbeit so wertvoll , wichtig und gut finde!

    betterplace.org/de/projects/12

    (Via Betterplace für bessere Sichtbarkeit - hab ich vorher via Mail abgeklärt) .

    #vem