#fibsem — Public Fediverse posts

@innuendo @jernej__s @ZachWeinersmith

Our eFIB-SEM (as per Xu et al. 2017 https://elifesciences.org/articles/25916 ) runs 24/7 for up to 8 weeks, when it runs out of source. And it's essential that it does so, uninterrupted, since it's imaging at nanometre resolution, milling 2 nm slices at a time with an ion beam and then repeatedly imaging the block face. The process is unsurprisingly very sensitive; was hard enough to overcome the reheat cycle of the instrument itself (every 60 hours or so) by detecting it, de-engaging the beams and then seamlessly re-engaging with the block face.

A brief video of the kind of data we acquire:
https://youtu.be/p4MH4-I-P5I

The MS Windows computer deciding to reboot on its own is unacceptable.

#ElectronMicroscopy #Zeiss #FIBSEM #VolumeEM #vEM #Drosophila

Postdoc position at the lab of Michael Winding at #TheCrick in London:

https://crick.wd3.myworkdayjobs.com/External/job/London/Postdoctoral-Fellow---M-Winding-Social-Circuits-and-Connectomics-Laboratory_R1447-1

... on neuronal circuits that drive social interactions between animals.

#neuroscience #connectomics #Drosophila #vEM #FIBSEM

#introduction

Hi fellow humans, I am Laura (zie/zir if possible, otherwise she/her), a senior staff scientist at #LSFOctopus advanced #LightMicroscopy and #CLEM facility at #HarwellCampus in the #UK, right next to #DiamondLightSource.

My science interest center around #EGFRSignalling and #RTKSignalling and deregulation in #CancerStudies in general. My tools of the trade are #singleparticletracking #singlemoleculelocalisation #CLEM #FIBSEM #VolumeEM and #CryoMicroscopy

HMU for science chat