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#cryoclem — Public Fediverse posts

Live and recent posts from across the Fediverse tagged #cryoclem, aggregated by home.social.

  1. #IINSPhD🧑‍🎓 Paul Lapios, PhD student in David Perrais' team, #MemTraS, defends his thesis: “Ultrastructural and molecular analysis of cortico-striatal dopamine hub synapses”

    He worked on the #synapse, #dopamine, synaptic plasticity, #striatum and #cryoCLEM

    📎bordeaux-neurocampus.fr/event/

  2. Turns out the postsynaptic densities (PSD) that we use in electron microscopy to identify synapses aren’t made of large protein aggregates but rather of specialised cellular organelles:

    “we combined mouse genetics and cryo-electron tomography to determine the 3D molecular architecture of fresh isolated and anatomically intact synapses in the adult forebrain. The native glutamatergic synapse did not consistently show a high density of proteins at the postsynaptic membrane thought to be characteristic of the PSD. Instead, a ‘synaptoplasm’ consisting of cytoskeletal elements, macromolecular complexes and membrane-bound organelles extended throughout the pre- and post-synaptic compartments.”

    Excellent work by Julia Peukes et al. 2024 elifesciences.org/reviewed-pre

    #neuroscience #synapses #CryoEM #CryoCLEM