#radseq — Public Fediverse posts
Live and recent posts from across the Fediverse tagged #radseq, aggregated by home.social.
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At the end of a long day of #bioinformatics I've confirmed that I can cut my sequencing depth by half in the next round of sampling. Which means costs drop by half as well!
By tomorrow we'll see if we can cut by another half again.
Motivated by wanting to do #RADseq / #GBS population #genetics work on an octoploid plant. ugh!
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What is the key element in a successful #RADseq experiment and analysis? How can you avoid the dreaded "missing data"?
tldr; it's the quality of input DNA, and controlling the resulting levels of PCR duplicates that matters.
Our latest work, led by Nicolas Rochette and Angel Rivera-Colón, (and in collaboration with Shane Campbell-Staton and Floragenex) shows this in great detail via modeling and experimental analysis. Now published in Molecular Ecology Resources:
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🥁Registration is now open for the next edition of the #RADseq #dataanalysis course with @naiara_re @TerezaManousaki & @NataliaDA10
Have a look at it: https://physalia-courses.org/courses-workshops/course16/
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To get the ball rolling, I'd like to share a recent paper! Genotyping-by-sequencing for biogeography: https://doi.org/10.1111/jbi.14516
We provide a practical guide for #biogeography using #GBS or #RADseq, which should particularly assist new users or students. There's a wealth of research exploring differences in reduced-representation sequencing (RRS) methods, sources of genotyping error, and much more. Our aim was to guide new users through this exciting literature. #genomics #ecology #evolution #DNA
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PhD position available at Lund University on conservation genomics of orchids in Sweden. Apply here: https://lu.varbi.com/en/what:job/jobID:557391/type:job/where:4/apply:1