#intron — Public Fediverse posts
Live and recent posts from across the Fediverse tagged #intron, aggregated by home.social.
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"Most importantly, by proving that the evolutionarily-conserved RNA core of splicing machines can recognize small molecules specifically, our work provides a solid basis for the rational design of splicing modulators not only against bacterial and organellar introns, but also against the human spliceosome, which is a validated drug target for the treatment of congenital diseases and cancers." #spliceosome #splicing #intron #introns #transcription #ribonucleicAcid #RNA
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"Evidence implicates..catalytic activity of the intron lariat spliceosome (ILS) in which the 3′-OH of the lariat tail... attacks the branch, joining the intron 3′ end to the 5′ splice site in a 3′–5′ linked circle. Human U2 and U12 spliceosomes produce analogous full-length and processed circles. Postsplicing catalytic activity of the #spliceosome may promote #intron transposition during eukaryotic #genome #evolution."
http://m.genesdev.cshlp.org/content/early/2024/05/09/gad.351764.124
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A new paper from @wu_xuebing 's lab just published @Nature
"Noncoding translation mitigation"https://www.nature.com/articles/s41586-023-05946-4
Here are my thoughts:
It is a very interesting paper, and important to anyone working on #translation, particularly from #ncRNAs, but not only.
In brief, they screened short sequences originating from #lncRNA or from non coding regions ( #UTR #intron) (and #mRNA CDS as control) which they fused to GFP at the C-term and looked at GFP expression level.
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Have any antisense oligonucleotide #ASO experts made it onto mastodon?
I'm trying to find evidence on how much of a role #intron secondary structure remediation plays in splice-switching ASO design. A presentation by an ASO researcher from a biotech stated that the #spliceosome recognizes intron #secstruct, particularly the intron 5' structure, but haven't found published evidence or hypothesis.
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Long-standing #genomics mystery about the origins of #introns explained in new study
https://phys.org/news/2022-11-long-standing-genomic-mystery-introns.html#TransposableElements drive #intron gain in diverse eukaryotes https://www.pnas.org/doi/10.1073/pnas.2209766119
The researchers believe that #introners (specialized #transposons) are the only likely explanation for intron burst events, in which thousands of introns show up in a #genome seemingly all at once, and they find evidence of this in species across the eukaryotic tree of life.