#structbio — Public Fediverse posts

^ if this paper is of interest. There's a related preprint from van der Groot, Dal Peraro and D’Angelo labs:

https://www.biorxiv.org/content/10.1101/2025.06.26.661665v1

#MembraneTraffic #structbio

This is cool. CryoEM showing COPI coat containing GOLPH3, together with functional data suggests the mechanism for cargo retention/transport.

The mechanistic basis of cargo selection during Golgi maturation

Taylor, Zubkov, Ciazynska et al.

https://doi.org/10.1101/2025.06.26.661719

#MembraneTraffic #structbio

James and Selena's paper is out in JCB!

We present a structural model of the interaction between TACC3 and ch-TOG. Then we discover Affimers that can inhibit this interaction. Expression of the Affimers in cells uncovered a new role for TACC3-ch-TOG in maintaining the pericentriolar material during mitosis.

https://doi.org/10.1083/jcb.202407002

#CellBiology #StructBio #cytoskeleton

Looks like cryo-ET studies of synapses are hot right now (pardon the pun). Another preprint, this time on dopaminergic terminals, looking at vesicle structures in situ.

https://www.biorxiv.org/content/10.1101/2024.04.15.589543v1

#CellBiology #structbio

and while we are at it, this other preprint uses cryo-ET on *native* synapses to look at vesicles in the terminal

https://www.biorxiv.org/content/10.1101/2024.04.01.587595v1
#structuralbiology #structbio #cellbiology

Wow! Preprint from the Rubinstein lab shows the structure of V-ATPase in synaptic vesicles. Synaptophysin is part of the V-ATPase!

https://www.biorxiv.org/content/10.1101/2024.04.01.587493v1

#structuralbiology #structbio #cellbiology

We have a #PhD available!! Are you interested in #cryoEM 🔬and #RNAstructure🧬? Then this might be for you: tinyurl.com/4uecm38r It's a 4-year funded position through @BBSRCWhiteRose; DM or email me for more details. #RNA #CryoEM #molecularbio #generegulation #Structbio #structuralbiology

We have a #PhD available!! Are you interested in #cryoEM 🔬and #RNAstructure🧬? Then this might be for you: tinyurl.com/4uecm38r It's a 4-year funded position through @BBSRCWhiteRose; DM or email me for more details. #RNA #CryoEM #molecularbio #generegulation #Structbio #structuralbiology

We have a #PhD available!! Are you interested in #cryoEM 🔬and #RNAstructure🧬? Then this might be for you: tinyurl.com/4uecm38r It's a 4-year funded position through @BBSRCWhiteRose; DM or email me for more details. #RNA #CryoEM #molecularbio #generegulation #Structbio #structuralbiology

We have a #PhD available!! Are you interested in #cryoEM 🔬and #RNAstructure🧬? Then this might be for you: tinyurl.com/4uecm38r It's a 4-year funded position through @BBSRCWhiteRose; DM or email me for more details. #RNA #CryoEM #molecularbio #generegulation #Structbio #structuralbiology

We have a #PhD available!! Are you interested in #cryoEM 🔬and #RNAstructure🧬? Then this might be for you: tinyurl.com/4uecm38r It's a 4-year funded position through @BBSRCWhiteRose; DM or email me for more details. #RNA #CryoEM #molecularbio #generegulation #Structbio #structuralbiology

Viral evolution and host co-evolution is my vice. I will go down that rabbit hole aimlessly off topic from what I need to be doing and come back none the wiser, just more questions and a bigger picture of things I do not know. #HIV # research #science #structbio #evolution

Negative stain: Wow, this protein is great! It's like shooting fish in a barrel

Cryo: Where did all the fish go? I swear I stocked all 6 barrels, 6 different ways, and then did that twice just to be sure. Not a single fish! I even used 3 different types of barrel

#structbio #cryoEM #gradschool #2lit2quit #virus

The best days #inthelab are when things don't work as expected and the result is even more interesting. I do not have time for this right now, but things are about to get real. #structbio
Side projects that become main projects are the best

Coming protein #macromolecular #crystallography #workshop in #Dubrovnik Coast (Croatia) April 16 to 21, 2023. https://htcc5.org/ 🇭🇷
Still participants slots available => do not hesitate to apply !
https://htcc5.org/2022/06/experimental-advances-in-macromolecular-crystallography/
#structbio #ccp4 #StrucuralBiology

New package! MRCFile.jl is a #JuliaLang parser for the MRC file format commonly used to store data like that produced by electron microscopy. #StructBio https://github.com/sethaxen/MRCFile.jl

I wrote this package for a project during my PhD and just now got around to registering it. I don't work with MRC files anymore, so if you do and are interested in co-maintaining, let me know!

Inhouse #structbio #xray #diffraction this afternoon. Three projects.
1) Hmm, those "spots" are very smeary, as before
2) A couple of interesting, but probably protein, spots from @xtaldave 's small crystals, so off to #DLS_MX #synchrotron they go.
3) Ohhh, better than 1.9 Å, so lets collect a quick dataset before sending off to #synchrotron.

#elonmusk may be a duchecanoe but #tesla does make some good batteries.

Unexpected power outage (no inclement weather) today, but the #structbio server stayed live!

#homelab

I have migrated my #Mastodon account to the new #structbio server:

https://struct.bio/@rdrighetto

Thanks @cryo2go for setting up the old and new servers and for the help with migration!

(also, testing cross-posting)

A quick look at the relentless pile of e-mails from during the end of year break.

You might think that the user, who was told that the function they wished to use in the crystal imaging software was broken, wouldn't try to use it on Boxing Day and then again on the New Year's BH but actually wait until being told it was fixed.

Problem is down to synchronisation issues migrating to new storage hardware so is unlikely to magically get better.

#Users #Christmas #IT #Structbio

It's not #structbio and it certainly isn't #cryoEM, but it's structure for sure and its cold, 3°C in this newly insulated and drywalled room. Hoping to start putting in HVAC asap
#weekendproject