#exm — Public Fediverse posts

Expansion microscopy with sub-molecular resolution:

"we present 1000ExM, a four-network IPN (interpenetrating polymer network) formed by an initial ionic gel and three successive ionic-in-ionic casting steps. This process yields ~1000-fold expansion in each dimension while preserving three-dimensional (3D) molecular geometry."

"Physical expansion converts sub-nanometer-scale molecular distances into micrometer-scale separations that are resolvable using standard light microscopy, enabling direct readout of 3D amino acid positions without the need for averaging."

"Thousandfold Expansion Microscopy", Hu et al. 2026 (Boyden lab)
https://www.biorxiv.org/content/10.64898/2026.05.31.729018v1

#ExM #microscopy

"Charting the landscape of cytoskeletal diversity in microbial eukaryotes", Mikus et al. 2025 (Dudin lab)
https://www.cell.com/cell/fulltext/S0092-8674(25)01127-4

CC @davi

#CellBiology #cytoskeleton #ExM

“Expansion microscopy gives you opportunities that aren’t available with anything else.” New @nature, Elie Dolgin reports on a decade of "expansion microscopy", #ExM, published a decade ago this week. https://www.nature.com/articles/d41586-025-00059-6

Today I feel like a plant, static but alive

#ArabidopsisRoot #microscopy #ExM

Good morning!! Cell biology rocks, isn't it?

#microscopy #exm #cellbiology

There some of my specialities, enjoy!

#imaging #microscopy #neuroscience #superesolution #expansion #exm

#ExM #microscopy #artinscience

#ExIGS: Combining #expansion #microscopy (#ExM) & in situ #genome #sequencing (#IGS) enables sequencing of #genomic #DNA & #superresolution localization of #nuclear #proteins in single #cells:

Preprint: https://doi.org/10.1101/2024.09.24.614614
#DIYbio #lab #instruments #NGS #fluorescence #imaging

#fluorescent #microscopy #ExM #celldivision

Have a nice weekend!
#fluorescentfriday #expansionmicroscopy #ExM

Image registration for light-microscopy at petabyte scale, an update of the #BigSticher for #FijiSc by @preibischs

https://github.com/JaneliaSciComp/BigStitcher-Spark

Ready for expansion microscopy #ExM approaches to mapping neural circuits and more.

#BioimageInformatics