#tetrodes — Public Fediverse posts

#NeuroMethods question:
What do you all (experimentalists or computational people) prefer as a format to store and share #Electrophysiology data? Either for Tetrodes or Probes? And why? 👀

Is NWB (Neuroscience Without Borders) the gold standard?

#Neuroscience #Tetrodes #Probes #DataManagement

@elduvelle_neuro

I don't think it would be possible to record a single neuron on multiple tetrodes. What is the separation between your tetrodes?

#NeuroMethods #Ephys #SpikeSorting #Tetrodes

If you do need cross-correlation code, there is mex'd cross-correlation code in MClust.

Ok maybe this is the best cluster space ever
(also, from an implant that’s almost 4 months old!)
#Tetrodes #NeuroRat #Hippocampus #PlaceCells

Upping my shuttle-making game with 3D-printed shuttle molds 😁 💪
#DriveBuilding #Tetrodes #Ephys

Do I know anyone using the #NeuroMouse version of the #ShuttleDrive?
(Small, 32 (or 16?) tetrodes version)

If so can you tell me what you think in terms of stability, recording quality etc?
https://open-ephys.org/shuttledrive

Edit: still looking for labs using this in 2025!

#OpenEphys #HyperDrive #Tetrodes #NeuroMethods

My #NeuroRat implanted… 7 months ago still has nice clusters!!
#TetrodePower
#Ephys #Neuroscience #Tetrodes #Hippocampus

Hmm maybe this one is better… not sure 🤔 😁
#Tetrode #Tetrodes #Hippocampus #Screening

#Neuroscience #Ephys question: anyone recognize these signals? The wavy thing? It’s supposedly in rat cortex (above hippocampus), when the rat is immobile, possibly sleepy. Recorded with #Tetrodes.
The x-axis scale for each of the two columns is 1s.
#Electrophysiology

What tags are you all following?
Here are mine but I’d like to add some more, particularly science-related:

#FediTips #Hippocampus #PlaceCells #Tetrodes #NatureIsBeautiful (yes… I’m the only one using it for now) #NeuroPaper #NeuroPaperThread #Neuroscience

Spike-sorting, before /after

This uses the automated method Klustakwick and then some manual refinement

#PlaceCells #SpikeSorting #Tetrodes #Neuroscience

#SpikeSorting : where would be the best place (or handle / hashtag) to ask questions about Phy? 🙏​
And if you use it, can you let me know, maybe we can help each other?
(This Phy: https://phy.readthedocs.io/en/latest/)
*Edit: added these hashtags: #Electrophysiology #Neuroscience #Tetrodes *

⤵️​ Giving the final touches to the #HybridDrive

In more details:

1. Adding the cone
   (painted with conductive epoxy, connected to the drive’s ground, to (try and) shield it from electrical interference, but it’s the first time I do this so I don’t know if it will work. Once on the rat, it will have a cap as well, except when plugged into the recording system.)

2. #GoldPlating
   This drive has 2 sets of tetrodes: 13 microns Nichrome wire and 17 microns Platinum-Iridium wire (testing which is best). Due to different wire material and diameter, they get gold-plated slightly differently; see this for a good plating tutorial for 12-13 um Nichrome, generously shared by John Bladon: https://github.com/elduvelle/ephys_tutorials/blob/main/4_gold_plating_Nichrome_12um.md

3. View of the finished drive.
   I am also testing the effects of implanting the tetrodes directly in the brain during surgery, or keeping them retracted in the drive and only lower them at the end of the surgery. This is for a bilateral dorsal #Hippocampus implant so it has two groups of tetrodes. I personally do not understand the point of not implanting directly in brain…

4. The guide/outer cannulas and their #Tetrodes
   Each guide cannula holds 4 tetrodes which move together, excepted 1 cannula that holds a single tetrode - to be left in the corpus callosum and used for reference. If everything works as planned. I used 27 gauge (thin walled) inner cannulas to guide the tetrodes - the smaller diameter that you can see); these do not move, only the shuttles and the tetrodes move.

Next step is implantation surgery!

2/
Here are some interesting tables from it...
I wonder how well it works with #Tetrodes and in the #Hippocampus given the special shape of #ComplexSpikes 🤔​

If you try it please let us know what you think!!

#Kilosort #Electrophysiology #SpikeSorting
2/

@chrisXrodgers
Very good question!
I have most recently used MountainSort + Phy (via SpikeInterface) and I quite like Phy (for manual refinement of clusters) but not so much Mountainsort - it seemed very conservative. I’ll go back to it soon and tweak the settings a bit. If you want to try it out we have some shared code with the Genzel lab here: https://github.com/elduvelle/SpikeinterfaceMS4_GenzelLab

But I was also going to try out Kilosort. Do you really not like it?
Before that KlustaKwik + Tint has been the gold standard for me :) … but maybe it’s time to move on!
#SpikeSorting #Tetrodes #Electrophysiology