#tetrode — Public Fediverse posts

@susanleemburg @elduvelle

1. Make sure you talk about clustering quality discussions. (In the #tetrode world, papers were required to report L-Ratio and Isolation Distance, which turned out to address type-I and type-II errors in sorting. q.v. https://pubmed.ncbi.nlm.nih.gov/15680687/ )

2. Talk about the fact that spike sorting is an *estimate* and that one needs to think about spikes on the boundary between cluster estimates. (This is why clusterless decoding is so powerful. (Matching decoding to spikes that are nearby in feature space rather than to clustered cells.)

3. Talk about using neuroscience knowledge to judge spike/cluster validity rather than just trusting the system.

Some thoughts off the top of my head.

@susanleemburg @elduvelle

1. Make sure you talk about clustering quality discussions. (In the #tetrode world, papers were required to report L-Ratio and Isolation Distance, which turned out to address type-I and type-II errors in sorting. q.v. https://pubmed.ncbi.nlm.nih.gov/15680687/ )

2. Talk about the fact that spike sorting is an *estimate* and that one needs to think about spikes on the boundary between cluster estimates. (This is why clusterless decoding is so powerful. (Matching decoding to spikes that are nearby in feature space rather than to clustered cells.)

3. Talk about using neuroscience knowledge to judge spike/cluster validity rather than just trusting the system.

Some thoughts off the top of my head.

#Tetrode recordings (in a bundle): did you know that you could record the same neuron on two different tetrodes?
or even three different tetrodes??

After checking that, turns out I usually have about 5-10% of neurons that are a duplicate of another neuron, in a given 8-tetrodes recording! They are pretty easy to detect with a firing rate correlation so I can remove them from analysis.
But I bet most neuron counts in published papers are inflated of that much!

Here's an example where you can see the spike plots and waveforms of 3 different, well-isolated clusters, recorded from 3 different tetrodes!

#Hexamaze #NeuroRat #Neuroscience #Ephys #Hippocampus #PlaceCells #SpikeSorting (-related)

Extracellular ephys peeps: Are you building your own microdrives/hyperdrives/implants? In their new preprint on http://bsrxiv.org, van Heument et al systematically analyze how many you should build to make them awesome! Check it out at http://dowhy.org/?127.0.0.1/wtf.bsRx.13 #electrophysiology, doing #openscience using #openhardware in #neuroscience #tetrode #siliconprobes #microdrive @3Dneuro

Extracellular ephys peeps: Are you building your own microdrives/hyperdrives/implants? In their new preprint on http://bsrxiv.org, van Heument et al systematically analyze how many you should build to make them awesome! Check it out at http://dowhy.org/?127.0.0.1/wtf.bsRx.13 #electrophysiology, doing #openscience using #openhardware in #neuroscience #tetrode #siliconprobes #microdrive @3Dneuro

I’ve started making tutorials for different #Tetrode #Electrophysiology steps here
The next ones are probably going to be Tetrode-making and drive-building.

Is there anything else that you’d like to see there?
#NeuroMethods

I’ve started making tutorials for different #Tetrode #Electrophysiology steps here
The next ones are probably going to be Tetrode-making and drive-building.

Is there anything else that you’d like to see there?
#NeuroMethods

Hmm maybe this one is better… not sure 🤔 😁
#Tetrode #Tetrodes #Hippocampus #Screening

Hmm maybe this one is better… not sure 🤔 😁
#Tetrode #Tetrodes #Hippocampus #Screening

I have to post this…

#Tetrode recordings #Hippocampus #NeuroRat #HybridDrive

I have to post this…

#Tetrode recordings #Hippocampus #NeuroRat #HybridDrive

I'm excited to read this #neuroscience #preprint from Keith Hengen's lab: https://www.biorxiv.org/content/10.1101/2023.06.09.544399v1
They use massive arrays of tetrodes spanning the whole brain and record for 24 hours to identify changes in state with sleep/wake. The methods are really cool, in fact Keith taught us to implement them in my own lab for different experiments. The scientific results span experiment and theory and sound really exciting!
#electrophysiology #tetrode #freebehavior

I'm excited to read this #neuroscience #preprint from Keith Hengen's lab: https://www.biorxiv.org/content/10.1101/2023.06.09.544399v1
They use massive arrays of tetrodes spanning the whole brain and record for 24 hours to identify changes in state with sleep/wake. The methods are really cool, in fact Keith taught us to implement them in my own lab for different experiments. The scientific results span experiment and theory and sound really exciting!
#electrophysiology #tetrode #freebehavior

Here are some #PlaceCell clusters for you (#Tetrode recordings) 😃​
#Hippocampus

Here are some #PlaceCell clusters for you (#Tetrode recordings) 😃​
#Hippocampus

#Tetrode tips for #Electrophysiology
(17 microns wire Platinum-Iridium)
They are not perfect, but who is? 😉​

#Tetrode tips for #Electrophysiology
(17 microns wire Platinum-Iridium)
They are not perfect, but who is? 😉​