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#tissueclearing — Public Fediverse posts

Live and recent posts from across the Fediverse tagged #tissueclearing, aggregated by home.social.

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  1. Do you already have cleared-brain image stacks, but don't know what to do with them? Or maybe you want to learn which parameters are important before embarking on an imaging campaign? In this intensive 2-day hands-on workshop you will learn how to handle 3D #lightsheetmicroscopy data, how to work with the DELiVR pipeline, and how to create your own annotations in #VR.
    September 9-10th, at the Ertürk lab at @HelmholtzMunich in Munich, Germany.
    (1/2)
    #neuroscience #tissueclearing #imageanalysis

  2. Do you already have cleared-brain image stacks, but don't know what to do with them? Or maybe you want to learn which parameters are important before embarking on an imaging campaign? In this intensive 2-day hands-on workshop you will learn how to handle 3D #lightsheetmicroscopy data, how to work with the DELiVR pipeline, and how to create your own annotations in #VR.
    September 9-10th, at the Ertürk lab at @HelmholtzMunich in Munich, Germany.
    (1/2)
    #neuroscience #tissueclearing #imageanalysis

  3. Do you already have cleared-brain image stacks, but don't know what to do with them? Or maybe you want to learn which parameters are important before embarking on an imaging campaign? In this intensive 2-day hands-on workshop you will learn how to handle 3D #lightsheetmicroscopy data, how to work with the DELiVR pipeline, and how to create your own annotations in #VR.
    September 9-10th, at the Ertürk lab at @HelmholtzMunich in Munich, Germany.
    (1/2)
    #neuroscience #tissueclearing #imageanalysis

  4. Do you already have cleared-brain image stacks, but don't know what to do with them? Or maybe you want to learn which parameters are important before embarking on an imaging campaign? In this intensive 2-day hands-on workshop you will learn how to handle 3D #lightsheetmicroscopy data, how to work with the DELiVR pipeline, and how to create your own annotations in #VR.
    September 9-10th, at the Ertürk lab at @HelmholtzMunich in Munich, Germany.
    (1/2)
    #neuroscience #tissueclearing #imageanalysis

  5. Do you already have cleared-brain image stacks, but don't know what to do with them? Or maybe you want to learn which parameters are important before embarking on an imaging campaign? In this intensive 2-day hands-on workshop you will learn how to handle 3D #lightsheetmicroscopy data, how to work with the DELiVR pipeline, and how to create your own annotations in #VR.
    September 9-10th, at the Ertürk lab at @HelmholtzMunich in Munich, Germany.
    (1/2)
    #neuroscience #tissueclearing #imageanalysis

  6. @brainglobe @SWC_Neuro This is excellent news! We're currently piloting rat brain clearing & light-sheet imaging, so this atlas will be very welcome.
    #lightsheetmicroscopy #tissueclearing #rodent #neuroanatomy #neuroscience

  7. @brainglobe @SWC_Neuro This is excellent news! We're currently piloting rat brain clearing & light-sheet imaging, so this atlas will be very welcome.
    #lightsheetmicroscopy #tissueclearing #rodent #neuroanatomy #neuroscience

  8. @brainglobe @SWC_Neuro This is excellent news! We're currently piloting rat brain clearing & light-sheet imaging, so this atlas will be very welcome.
    #lightsheetmicroscopy #tissueclearing #rodent #neuroanatomy #neuroscience

  9. @brainglobe @SWC_Neuro This is excellent news! We're currently piloting rat brain clearing & light-sheet imaging, so this atlas will be very welcome.
    #lightsheetmicroscopy #tissueclearing #rodent #neuroanatomy #neuroscience

  10. @brainglobe @SWC_Neuro This is excellent news! We're currently piloting rat brain clearing & light-sheet imaging, so this atlas will be very welcome.
    #lightsheetmicroscopy #tissueclearing #rodent #neuroanatomy #neuroscience

  11. This week I had the pleasure of attending the #MesoSPIM Symposium in Zürich, Switzerland - and what an experience it's been! Top-notch presentations, great posters and great chats with my fellow #lightsheet #microscopy and #tissueclearing nerds. You know it's a good conference when you come back with a full notebook and a head fizzing with ideas on what to do next.
    I could make the night train journey there thanks to @Co_Biologists's DMM Conference Travel Grant - much appreciated!

  12. This week I had the pleasure of attending the #MesoSPIM Symposium in Zürich, Switzerland - and what an experience it's been! Top-notch presentations, great posters and great chats with my fellow #lightsheet #microscopy and #tissueclearing nerds. You know it's a good conference when you come back with a full notebook and a head fizzing with ideas on what to do next.
    I could make the night train journey there thanks to @Co_Biologists's DMM Conference Travel Grant - much appreciated!

  13. This week I had the pleasure of attending the #MesoSPIM Symposium in Zürich, Switzerland - and what an experience it's been! Top-notch presentations, great posters and great chats with my fellow #lightsheet #microscopy and #tissueclearing nerds. You know it's a good conference when you come back with a full notebook and a head fizzing with ideas on what to do next.
    I could make the night train journey there thanks to @Co_Biologists's DMM Conference Travel Grant - much appreciated!

  14. This week I had the pleasure of attending the #MesoSPIM Symposium in Zürich, Switzerland - and what an experience it's been! Top-notch presentations, great posters and great chats with my fellow #lightsheet #microscopy and #tissueclearing nerds. You know it's a good conference when you come back with a full notebook and a head fizzing with ideas on what to do next.
    I could make the night train journey there thanks to @Co_Biologists's DMM Conference Travel Grant - much appreciated!

  15. This week I had the pleasure of attending the #MesoSPIM Symposium in Zürich, Switzerland - and what an experience it's been! Top-notch presentations, great posters and great chats with my fellow #lightsheet #microscopy and #tissueclearing nerds. You know it's a good conference when you come back with a full notebook and a head fizzing with ideas on what to do next.
    I could make the night train journey there thanks to @Co_Biologists's DMM Conference Travel Grant - much appreciated!

  16. 🔬📄 'Efficacy and Feasibility of Tissue-Clearing Technique and Three-Dimensional Imaging in the Human Gastrointestinal Tissues Using Illuminate Cleared Organs to Identify Target Molecules' - a Karger: #Gastroenterology article on #ScienceOpen -

    🔗 scienceopen.com/document?vid=0

    #TissueClearing #3DImaging #LUCIDProtocol #Pathology #Microscopy

  17. 🔬📄 'Efficacy and Feasibility of Tissue-Clearing Technique and Three-Dimensional Imaging in the Human Gastrointestinal Tissues Using Illuminate Cleared Organs to Identify Target Molecules' - a Karger: #Gastroenterology article on #ScienceOpen -

    🔗 scienceopen.com/document?vid=0

    #TissueClearing #3DImaging #LUCIDProtocol #Pathology #Microscopy

  18. 🔬📄 'Efficacy and Feasibility of Tissue-Clearing Technique and Three-Dimensional Imaging in the Human Gastrointestinal Tissues Using Illuminate Cleared Organs to Identify Target Molecules' - a Karger: #Gastroenterology article on #ScienceOpen -

    🔗 scienceopen.com/document?vid=0

    #TissueClearing #3DImaging #LUCIDProtocol #Pathology #Microscopy

  19. Here's an interesting way to do photobleaching with cleared tissue: Add 0.1% H2O2 to the clearing medium and blast with LEDs for 24h at RT. Also includes very detailed instructions for building an LED photobleaching setup - the samples ate placed between two PCBs with LEDs in a ventilated box.

    Open-source Photobleacher for Fluorescent Imaging of Large Pigment-Rich Tissues
    Murakami et al., preprint at biorxiv 2025
    biorxiv.org/content/10.1101/20

    #tissueclearing #fluorescence #microscopy #neuroscience

  20. Here's an interesting way to do photobleaching with cleared tissue: Add 0.1% H2O2 to the clearing medium and blast with LEDs for 24h at RT. Also includes very detailed instructions for building an LED photobleaching setup - the samples ate placed between two PCBs with LEDs in a ventilated box.

    Open-source Photobleacher for Fluorescent Imaging of Large Pigment-Rich Tissues
    Murakami et al., preprint at biorxiv 2025
    biorxiv.org/content/10.1101/20

    #tissueclearing #fluorescence #microscopy #neuroscience

  21. Here's an interesting way to do photobleaching with cleared tissue: Add 0.1% H2O2 to the clearing medium and blast with LEDs for 24h at RT. Also includes very detailed instructions for building an LED photobleaching setup - the samples ate placed between two PCBs with LEDs in a ventilated box.

    Open-source Photobleacher for Fluorescent Imaging of Large Pigment-Rich Tissues
    Murakami et al., preprint at biorxiv 2025
    biorxiv.org/content/10.1101/20

    #tissueclearing #fluorescence #microscopy #neuroscience

  22. Here's an interesting way to do photobleaching with cleared tissue: Add 0.1% H2O2 to the clearing medium and blast with LEDs for 24h at RT. Also includes very detailed instructions for building an LED photobleaching setup - the samples ate placed between two PCBs with LEDs in a ventilated box.

    Open-source Photobleacher for Fluorescent Imaging of Large Pigment-Rich Tissues
    Murakami et al., preprint at biorxiv 2025
    biorxiv.org/content/10.1101/20

    #tissueclearing #fluorescence #microscopy #neuroscience

  23. Here's an interesting way to do photobleaching with cleared tissue: Add 0.1% H2O2 to the clearing medium and blast with LEDs for 24h at RT. Also includes very detailed instructions for building an LED photobleaching setup - the samples ate placed between two PCBs with LEDs in a ventilated box.

    Open-source Photobleacher for Fluorescent Imaging of Large Pigment-Rich Tissues
    Murakami et al., preprint at biorxiv 2025
    biorxiv.org/content/10.1101/20

    #tissueclearing #fluorescence #microscopy #neuroscience

  24. New publication from Kwanghun Chung lab at MIT improves on their SWITCH approach and eFLASH stochastic electrotransport to improve antibody labeling by gradually shifting the microenvironment. Looks interesting, I wonder how well it can be adapted to other labeling protocols.

    Uniform volumetric single-cell processing for organ-scale molecular phenotyping
    Yun et al., Nature Biotechnology 2025
    doi.org/10.1038/s41587-024-025

    #neuroscience #tissueclearing #lightsheet #microscopy #fluorescenceFriday

  25. New publication from Kwanghun Chung lab at MIT improves on their SWITCH approach and eFLASH stochastic electrotransport to improve antibody labeling by gradually shifting the microenvironment. Looks interesting, I wonder how well it can be adapted to other labeling protocols.

    Uniform volumetric single-cell processing for organ-scale molecular phenotyping
    Yun et al., Nature Biotechnology 2025
    doi.org/10.1038/s41587-024-025

    #neuroscience #tissueclearing #lightsheet #microscopy #fluorescenceFriday

  26. New publication from Kwanghun Chung lab at MIT improves on their SWITCH approach and eFLASH stochastic electrotransport to improve antibody labeling by gradually shifting the microenvironment. Looks interesting, I wonder how well it can be adapted to other labeling protocols.

    Uniform volumetric single-cell processing for organ-scale molecular phenotyping
    Yun et al., Nature Biotechnology 2025
    doi.org/10.1038/s41587-024-025

    #neuroscience #tissueclearing #lightsheet #microscopy #fluorescenceFriday

  27. New publication from Kwanghun Chung lab at MIT improves on their SWITCH approach and eFLASH stochastic electrotransport to improve antibody labeling by gradually shifting the microenvironment. Looks interesting, I wonder how well it can be adapted to other labeling protocols.

    Uniform volumetric single-cell processing for organ-scale molecular phenotyping
    Yun et al., Nature Biotechnology 2025
    doi.org/10.1038/s41587-024-025

    #neuroscience #tissueclearing #lightsheet #microscopy #fluorescenceFriday

  28. New publication from Kwanghun Chung lab at MIT improves on their SWITCH approach and eFLASH stochastic electrotransport to improve antibody labeling by gradually shifting the microenvironment. Looks interesting, I wonder how well it can be adapted to other labeling protocols.

    Uniform volumetric single-cell processing for organ-scale molecular phenotyping
    Yun et al., Nature Biotechnology 2025
    doi.org/10.1038/s41587-024-025

    #neuroscience #tissueclearing #lightsheet #microscopy #fluorescenceFriday

  29. Wow, TRISCO (née TRIC-DISCO) is out in Science! This cool approach allows imaging of mRNA transcripts throughout the cleared mouse brain. It uses a signal amplification step with in situ Hybridization Chain Reaction (isHCR) and combines it with DISCO-style solvent-based #tissueclearing to make the brain transparent.

    Whole-brain spatial transcriptional analysis at cellular resolution
    Kanatani et al., Science 2024
    doi.org/10.1126/science.adn994

    #neuroscience #lightsheet #microscopy #isHCR #mRNA #TRISCO

  30. Wow, TRISCO (née TRIC-DISCO) is out in Science! This cool approach allows imaging of mRNA transcripts throughout the cleared mouse brain. It uses a signal amplification step with in situ Hybridization Chain Reaction (isHCR) and combines it with DISCO-style solvent-based #tissueclearing to make the brain transparent.

    Whole-brain spatial transcriptional analysis at cellular resolution
    Kanatani et al., Science 2024
    doi.org/10.1126/science.adn994

    #neuroscience #lightsheet #microscopy #isHCR #mRNA #TRISCO

  31. Wow, TRISCO (née TRIC-DISCO) is out in Science! This cool approach allows imaging of mRNA transcripts throughout the cleared mouse brain. It uses a signal amplification step with in situ Hybridization Chain Reaction (isHCR) and combines it with DISCO-style solvent-based #tissueclearing to make the brain transparent.

    Whole-brain spatial transcriptional analysis at cellular resolution
    Kanatani et al., Science 2024
    doi.org/10.1126/science.adn994

    #neuroscience #lightsheet #microscopy #isHCR #mRNA #TRISCO

  32. Wow, TRISCO (née TRIC-DISCO) is out in Science! This cool approach allows imaging of mRNA transcripts throughout the cleared mouse brain. It uses a signal amplification step with in situ Hybridization Chain Reaction (isHCR) and combines it with DISCO-style solvent-based #tissueclearing to make the brain transparent.

    Whole-brain spatial transcriptional analysis at cellular resolution
    Kanatani et al., Science 2024
    doi.org/10.1126/science.adn994

    #neuroscience #lightsheet #microscopy #isHCR #mRNA #TRISCO

  33. Wow, TRISCO (née TRIC-DISCO) is out in Science! This cool approach allows imaging of mRNA transcripts throughout the cleared mouse brain. It uses a signal amplification step with in situ Hybridization Chain Reaction (isHCR) and combines it with DISCO-style solvent-based #tissueclearing to make the brain transparent.

    Whole-brain spatial transcriptional analysis at cellular resolution
    Kanatani et al., Science 2024
    doi.org/10.1126/science.adn994

    #neuroscience #lightsheet #microscopy #isHCR #mRNA #TRISCO

  34. Clever stacking of labels allows the retention of cytoskeleton in tissues (here: Shark skin):

    Phalloidin-Alexa488 -> Anti-Alexa488 primary antibody -> Secondary antibody -> Hydrogel crosslinking -> ProtK tissue digestion -> Clearing

    Whole Tissue Imaging of Cellular Boundaries at Sub-Micron Resolutions for Automatic Cell Segmentation: Applications in Epithelial Bending of Ectodermal Appendages
    Norris et al., preprint at biorxiv 2024
    doi.org/10.1101/2024.06.26.600

    #tissueclearing #microscopy #sharks

  35. Clever stacking of labels allows the retention of cytoskeleton in tissues (here: Shark skin):

    Phalloidin-Alexa488 -> Anti-Alexa488 primary antibody -> Secondary antibody -> Hydrogel crosslinking -> ProtK tissue digestion -> Clearing

    Whole Tissue Imaging of Cellular Boundaries at Sub-Micron Resolutions for Automatic Cell Segmentation: Applications in Epithelial Bending of Ectodermal Appendages
    Norris et al., preprint at biorxiv 2024
    doi.org/10.1101/2024.06.26.600

    #tissueclearing #microscopy #sharks

  36. Clever stacking of labels allows the retention of cytoskeleton in tissues (here: Shark skin):

    Phalloidin-Alexa488 -> Anti-Alexa488 primary antibody -> Secondary antibody -> Hydrogel crosslinking -> ProtK tissue digestion -> Clearing

    Whole Tissue Imaging of Cellular Boundaries at Sub-Micron Resolutions for Automatic Cell Segmentation: Applications in Epithelial Bending of Ectodermal Appendages
    Norris et al., preprint at biorxiv 2024
    doi.org/10.1101/2024.06.26.600

    #tissueclearing #microscopy #sharks

  37. Clever stacking of labels allows the retention of cytoskeleton in tissues (here: Shark skin):

    Phalloidin-Alexa488 -> Anti-Alexa488 primary antibody -> Secondary antibody -> Hydrogel crosslinking -> ProtK tissue digestion -> Clearing

    Whole Tissue Imaging of Cellular Boundaries at Sub-Micron Resolutions for Automatic Cell Segmentation: Applications in Epithelial Bending of Ectodermal Appendages
    Norris et al., preprint at biorxiv 2024
    doi.org/10.1101/2024.06.26.600

    #tissueclearing #microscopy #sharks

  38. Clever stacking of labels allows the retention of cytoskeleton in tissues (here: Shark skin):

    Phalloidin-Alexa488 -> Anti-Alexa488 primary antibody -> Secondary antibody -> Hydrogel crosslinking -> ProtK tissue digestion -> Clearing

    Whole Tissue Imaging of Cellular Boundaries at Sub-Micron Resolutions for Automatic Cell Segmentation: Applications in Epithelial Bending of Ectodermal Appendages
    Norris et al., preprint at biorxiv 2024
    doi.org/10.1101/2024.06.26.600

    #tissueclearing #microscopy #sharks

  39. Interesting, though complex, signal amplification strategy using Nanobodies coupled to peroxidase -> added Glucose Peroxidase -> (reaction glucose -> H2O2) -> tyramide-based signal amplification -> fluorophore deposition.

    A Three Dimensional Immunolabeling Method with Peroxidase-fused Nanobodies and Fluorochromized Tyramide-Glucose Oxidase Signal Amplification
    Yamauchi et al., preprint at biorxiv 2024
    doi.org/10.1101/2024.10.25.620

    #tissueclearing #immunostaining #neuroscience #microscopy

  40. Interesting, though complex, signal amplification strategy using Nanobodies coupled to peroxidase -> added Glucose Peroxidase -> (reaction glucose -> H2O2) -> tyramide-based signal amplification -> fluorophore deposition.

    A Three Dimensional Immunolabeling Method with Peroxidase-fused Nanobodies and Fluorochromized Tyramide-Glucose Oxidase Signal Amplification
    Yamauchi et al., preprint at biorxiv 2024
    doi.org/10.1101/2024.10.25.620

    #tissueclearing #immunostaining #neuroscience #microscopy

  41. Interesting, though complex, signal amplification strategy using Nanobodies coupled to peroxidase -> added Glucose Peroxidase -> (reaction glucose -> H2O2) -> tyramide-based signal amplification -> fluorophore deposition.

    A Three Dimensional Immunolabeling Method with Peroxidase-fused Nanobodies and Fluorochromized Tyramide-Glucose Oxidase Signal Amplification
    Yamauchi et al., preprint at biorxiv 2024
    doi.org/10.1101/2024.10.25.620

    #tissueclearing #immunostaining #neuroscience #microscopy

  42. Interesting, though complex, signal amplification strategy using Nanobodies coupled to peroxidase -> added Glucose Peroxidase -> (reaction glucose -> H2O2) -> tyramide-based signal amplification -> fluorophore deposition.

    A Three Dimensional Immunolabeling Method with Peroxidase-fused Nanobodies and Fluorochromized Tyramide-Glucose Oxidase Signal Amplification
    Yamauchi et al., preprint at biorxiv 2024
    doi.org/10.1101/2024.10.25.620

    #tissueclearing #immunostaining #neuroscience #microscopy

  43. Interesting, though complex, signal amplification strategy using Nanobodies coupled to peroxidase -> added Glucose Peroxidase -> (reaction glucose -> H2O2) -> tyramide-based signal amplification -> fluorophore deposition.

    A Three Dimensional Immunolabeling Method with Peroxidase-fused Nanobodies and Fluorochromized Tyramide-Glucose Oxidase Signal Amplification
    Yamauchi et al., preprint at biorxiv 2024
    doi.org/10.1101/2024.10.25.620

    #tissueclearing #immunostaining #neuroscience #microscopy

  44. In case anyone was following the recent Science paper about using Tartrazine for #tissueclearing, apparently there is at least one (competing) group that couldn't reproduce it and wrote a preprint about it:

    Tartrazine cannot make live tissues transparent
    doi.org/10.1101/2024.09.29.615

    Discussion on Pubpeer, including the Tartrazine paper's author's response:
    pubpeer.com/publications/81314

    #lightsheet #microscopy #tartrazine

  45. In case anyone was following the recent Science paper about using Tartrazine for #tissueclearing, apparently there is at least one (competing) group that couldn't reproduce it and wrote a preprint about it:

    Tartrazine cannot make live tissues transparent
    doi.org/10.1101/2024.09.29.615

    Discussion on Pubpeer, including the Tartrazine paper's author's response:
    pubpeer.com/publications/81314

    #lightsheet #microscopy #tartrazine

  46. In case anyone was following the recent Science paper about using Tartrazine for #tissueclearing, apparently there is at least one (competing) group that couldn't reproduce it and wrote a preprint about it:

    Tartrazine cannot make live tissues transparent
    doi.org/10.1101/2024.09.29.615

    Discussion on Pubpeer, including the Tartrazine paper's author's response:
    pubpeer.com/publications/81314

    #lightsheet #microscopy #tartrazine

  47. In case anyone was following the recent Science paper about using Tartrazine for #tissueclearing, apparently there is at least one (competing) group that couldn't reproduce it and wrote a preprint about it:

    Tartrazine cannot make live tissues transparent
    doi.org/10.1101/2024.09.29.615

    Discussion on Pubpeer, including the Tartrazine paper's author's response:
    pubpeer.com/publications/81314

    #lightsheet #microscopy #tartrazine

  48. In case anyone was following the recent Science paper about using Tartrazine for #tissueclearing, apparently there is at least one (competing) group that couldn't reproduce it and wrote a preprint about it:

    Tartrazine cannot make live tissues transparent
    doi.org/10.1101/2024.09.29.615

    Discussion on Pubpeer, including the Tartrazine paper's author's response:
    pubpeer.com/publications/81314

    #lightsheet #microscopy #tartrazine

  49. New solvent-based #tissueclearing protocol claims reduced tissue distortion: Dehydration/delipidation with Hexanediol, tert-Butanol and N-butyldiethanolamine, and clearing with Benzyl Benzoate / PEGMA / N-butyldiethanolamine.

    SOLID: minimizing tissue distortion for brain-wide profiling of diverse architectures
    Zhu et al., Nature Comms 2024
    doi.org/10.1038/s41467-024-525

    #tissueclearing #neuroscience #lightsheet #microscopy

  50. New solvent-based #tissueclearing protocol claims reduced tissue distortion: Dehydration/delipidation with Hexanediol, tert-Butanol and N-butyldiethanolamine, and clearing with Benzyl Benzoate / PEGMA / N-butyldiethanolamine.

    SOLID: minimizing tissue distortion for brain-wide profiling of diverse architectures
    Zhu et al., Nature Comms 2024
    doi.org/10.1038/s41467-024-525

    #tissueclearing #neuroscience #lightsheet #microscopy

  51. New solvent-based #tissueclearing protocol claims reduced tissue distortion: Dehydration/delipidation with Hexanediol, tert-Butanol and N-butyldiethanolamine, and clearing with Benzyl Benzoate / PEGMA / N-butyldiethanolamine.

    SOLID: minimizing tissue distortion for brain-wide profiling of diverse architectures
    Zhu et al., Nature Comms 2024
    doi.org/10.1038/s41467-024-525

    #tissueclearing #neuroscience #lightsheet #microscopy

  52. New solvent-based #tissueclearing protocol claims reduced tissue distortion: Dehydration/delipidation with Hexanediol, tert-Butanol and N-butyldiethanolamine, and clearing with Benzyl Benzoate / PEGMA / N-butyldiethanolamine.

    SOLID: minimizing tissue distortion for brain-wide profiling of diverse architectures
    Zhu et al., Nature Comms 2024
    doi.org/10.1038/s41467-024-525

    #tissueclearing #neuroscience #lightsheet #microscopy

  53. New solvent-based #tissueclearing protocol claims reduced tissue distortion: Dehydration/delipidation with Hexanediol, tert-Butanol and N-butyldiethanolamine, and clearing with Benzyl Benzoate / PEGMA / N-butyldiethanolamine.

    SOLID: minimizing tissue distortion for brain-wide profiling of diverse architectures
    Zhu et al., Nature Comms 2024
    doi.org/10.1038/s41467-024-525

    #tissueclearing #neuroscience #lightsheet #microscopy

  54. Adding Dextran to ACSF helps with making slices (and living mouse brains in vivo) translucent and allows deeper and more precise imaging.

    In Vivo Optical Clearing of Mammalian Brain
    Franzesi et al., preprint at biorxiv 2024
    doi.org/10.1101/2024.09.05.611

    #neuroscience #invivo #tissueclearing #microscopy

  55. Adding Dextran to ACSF helps with making slices (and living mouse brains in vivo) translucent and allows deeper and more precise imaging.

    In Vivo Optical Clearing of Mammalian Brain
    Franzesi et al., preprint at biorxiv 2024
    doi.org/10.1101/2024.09.05.611

    #neuroscience #invivo #tissueclearing #microscopy

  56. Adding Dextran to ACSF helps with making slices (and living mouse brains in vivo) translucent and allows deeper and more precise imaging.

    In Vivo Optical Clearing of Mammalian Brain
    Franzesi et al., preprint at biorxiv 2024
    doi.org/10.1101/2024.09.05.611

    #neuroscience #invivo #tissueclearing #microscopy

  57. Adding Dextran to ACSF helps with making slices (and living mouse brains in vivo) translucent and allows deeper and more precise imaging.

    In Vivo Optical Clearing of Mammalian Brain
    Franzesi et al., preprint at biorxiv 2024
    doi.org/10.1101/2024.09.05.611

    #neuroscience #invivo #tissueclearing #microscopy

  58. Adding Dextran to ACSF helps with making slices (and living mouse brains in vivo) translucent and allows deeper and more precise imaging.

    In Vivo Optical Clearing of Mammalian Brain
    Franzesi et al., preprint at biorxiv 2024
    doi.org/10.1101/2024.09.05.611

    #neuroscience #invivo #tissueclearing #microscopy

  59. New #tissueclearing #preprint uses a custom mouse behaviour + deep-learning c-Fos analysis pipeline to compare several different Serotonin receptor agonists / antagonists in mice.

    Concerted modulation of spontaneous behavior and time-integrated whole-brain neuronal activity by serotonin receptors
    Friedmann et al., preprint at biorxiv 2024
    doi.org/10.1101/2024.08.02.606

    #lightsheet #microscopy #serotonin #neuroscience #preprint #brainmapping

  60. New #tissueclearing #preprint uses a custom mouse behaviour + deep-learning c-Fos analysis pipeline to compare several different Serotonin receptor agonists / antagonists in mice.

    Concerted modulation of spontaneous behavior and time-integrated whole-brain neuronal activity by serotonin receptors
    Friedmann et al., preprint at biorxiv 2024
    doi.org/10.1101/2024.08.02.606

    #lightsheet #microscopy #serotonin #neuroscience #preprint #brainmapping