#minknow — Public Fediverse posts

In order to enable #remote #access to #MinKNOW you need to

1. enable remote access on the sequencing computer using

```bash
sudo /opt/ont/minknow/bin/enable_remote_access.sh
sudo service minknow restart
```

2. install client on your personal computer

```bash
sudo apt install ont-kingfisher-ui-minion
```

3. log in in the client with your @nanopore account (it won't work for guest session).

4. and Add host using it's IP or (hostname if it's saved in `/etc/hosts`)

#bioinformatics #tips

In order to enable #remote #access to #MinKNOW you need to

1. enable remote access on the sequencing computer using

```bash
sudo /opt/ont/minknow/bin/enable_remote_access.sh
sudo service minknow restart
```

2. install client on your personal computer

```bash
sudo apt install ont-kingfisher-ui-minion
```

3. log in in the client with your @nanopore account (it won't work for guest session).

4. and Add host using it's IP or (hostname if it's saved in `/etc/hosts`)

#bioinformatics #tips

In order to enable #remote #access to #MinKNOW you need to

1. enable remote access on the sequencing computer using

```bash
sudo /opt/ont/minknow/bin/enable_remote_access.sh
sudo service minknow restart
```

2. install client on your personal computer

```bash
sudo apt install ont-kingfisher-ui-minion
```

3. log in in the client with your @nanopore account (it won't work for guest session).

4. and Add host using it's IP or (hostname if it's saved in `/etc/hosts`)

#bioinformatics #tips

In order to enable #remote #access to #MinKNOW you need to

1. enable remote access on the sequencing computer using

```bash
sudo /opt/ont/minknow/bin/enable_remote_access.sh
sudo service minknow restart
```

2. install client on your personal computer

```bash
sudo apt install ont-kingfisher-ui-minion
```

3. log in in the client with your @nanopore account (it won't work for guest session).

4. and Add host using it's IP or (hostname if it's saved in `/etc/hosts`)

#bioinformatics #tips

In order to enable #remote #access to #MinKNOW you need to

1. enable remote access on the sequencing computer using

```bash
sudo /opt/ont/minknow/bin/enable_remote_access.sh
sudo service minknow restart
```

2. install client on your personal computer

```bash
sudo apt install ont-kingfisher-ui-minion
```

3. log in in the client with your @nanopore account (it won't work for guest session).

4. and Add host using it's IP or (hostname if it's saved in `/etc/hosts`)

#bioinformatics #tips

[Перевод] Прочитал свой геном на кухне и превратил мазок щеки в 30 гигабаз данных ДНК

Автор прочитал свой геном прямо на кухонном столе. В статье — полный протокол без воды: какое железо купить, как не слить бюджет на проточную ячейку и как настроить таргетное обогащение, чтобы получить ответы именно по вашим вопросам. Переходите, если хотите понять, как устроена современная DIY-генетика и с чего начать свой первый прогон. Перейти к гайду

https://habr.com/ru/companies/flant/articles/1031594/

#секвенирование #геном #проточная_ячейка #minion #MinKNOW #лигирование #днк #спинколонка #Aтейлинг

[Перевод] Прочитал свой геном на кухне и превратил мазок щеки в 30 гигабаз данных ДНК

Автор прочитал свой геном прямо на кухонном столе. В статье — полный протокол без воды: какое железо купить, как не слить бюджет на проточную ячейку и как настроить таргетное обогащение, чтобы получить ответы именно по вашим вопросам. Переходите, если хотите понять, как устроена современная DIY-генетика и с чего начать свой первый прогон. Перейти к гайду

https://habr.com/ru/companies/flant/articles/1031594/

#секвенирование #геном #проточная_ячейка #minion #MinKNOW #лигирование #днк #спинколонка #Aтейлинг

[Перевод] Прочитал свой геном на кухне и превратил мазок щеки в 30 гигабаз данных ДНК

Автор прочитал свой геном прямо на кухонном столе. В статье — полный протокол без воды: какое железо купить, как не слить бюджет на проточную ячейку и как настроить таргетное обогащение, чтобы получить ответы именно по вашим вопросам. Переходите, если хотите понять, как устроена современная DIY-генетика и с чего начать свой первый прогон. Перейти к гайду

https://habr.com/ru/companies/flant/articles/1031594/

#секвенирование #геном #проточная_ячейка #minion #MinKNOW #лигирование #днк #спинколонка #Aтейлинг

@nanopore DRS sequencing temperature was lowered to 30/31C (#P2 / #MinION) in #minknow v24.06 (and all recent versions) slowing down RNA helicase from 130 bps to 100-110 bps, lowering yield per hour and affecting signal readouts.
In effect, recent and older #RNA004 runs differ in base- and modification calling quality, possibly pA tail estimates.
This change was not widely announced to the community apart from a brief mention in software release notes!
https://community.nanoporetech.com/posts/software-release-24-06-for
#academicChatter

@nanopore DRS sequencing temperature was lowered to 30/31C (#P2 / #MinION) in #minknow v24.06 (and all recent versions) slowing down RNA helicase from 130 bps to 100-110 bps, lowering yield per hour and affecting signal readouts.
In effect, recent and older #RNA004 runs differ in base- and modification calling quality, possibly pA tail estimates.
This change was not widely announced to the community apart from a brief mention in software release notes!
https://community.nanoporetech.com/posts/software-release-24-06-for
#academicChatter

@nanopore DRS sequencing temperature was lowered to 30/31C (#P2 / #MinION) in #minknow v24.06 (and all recent versions) slowing down RNA helicase from 130 bps to 100-110 bps, lowering yield per hour and affecting signal readouts.
In effect, recent and older #RNA004 runs differ in base- and modification calling quality, possibly pA tail estimates.
This change was not widely announced to the community apart from a brief mention in software release notes!
https://community.nanoporetech.com/posts/software-release-24-06-for
#academicChatter

@nanopore DRS sequencing temperature was lowered to 30/31C (#P2 / #MinION) in #minknow v24.06 (and all recent versions) slowing down RNA helicase from 130 bps to 100-110 bps, lowering yield per hour and affecting signal readouts.
In effect, recent and older #RNA004 runs differ in base- and modification calling quality, possibly pA tail estimates.
This change was not widely announced to the community apart from a brief mention in software release notes!
https://community.nanoporetech.com/posts/software-release-24-06-for
#academicChatter

@nanopore DRS sequencing temperature was lowered to 30/31C (#P2 / #MinION) in #minknow v24.06 (and all recent versions) slowing down RNA helicase from 130 bps to 100-110 bps, lowering yield per hour and affecting signal readouts.
In effect, recent and older #RNA004 runs differ in base- and modification calling quality, possibly pA tail estimates.
This change was not widely announced to the community apart from a brief mention in software release notes!
https://community.nanoporetech.com/posts/software-release-24-06-for
#academicChatter