#minion — Public Fediverse posts
Live and recent posts from across the Fediverse tagged #minion, aggregated by home.social.
-
Und hier sieht #Donald aus wie Auric #Goldfinger plus Blofeld/Infantino als #Minion.
-
Und hier sieht #Donald aus wie Auric #Goldfinger plus Blofeld/Infantino als #Minion.
-
Und hier sieht #Donald aus wie Auric #Goldfinger plus Blofeld/Infantino als #Minion.
-
Und hier sieht #Donald aus wie Auric #Goldfinger plus Blofeld/Infantino als #Minion.
-
Und hier sieht #Donald aus wie Auric #Goldfinger plus Blofeld/Infantino als #Minion.
-
-
-
-
-
Due to lack of popular demand, I've removed the dependency on "fish shell" from my #commandLine #journal tools.
If interested, check out
https://codeberg.org/EdTheDev/minionNow anyone using Bash shell can just clone #minion and add it to the path and start writing and searching for notes.
If you're curious, contrast it with my previous version written in #Python, and then #PowerShell and #goLang.https://github.com/edthedev/minion
As I've gained expeirence, I can accomplish roughly the same outcomes with less lines of code and fewer dependencies.
-
Due to lack of popular demand, I've removed the dependency on "fish shell" from my #commandLine #journal tools.
If interested, check out
https://codeberg.org/EdTheDev/minionNow anyone using Bash shell can just clone #minion and add it to the path and start writing and searching for notes.
If you're curious, contrast it with my previous version written in #Python, and then #PowerShell and #goLang.https://github.com/edthedev/minion
As I've gained expeirence, I can accomplish roughly the same outcomes with less lines of code and fewer dependencies.
-
[Перевод] Прочитал свой геном на кухне и превратил мазок щеки в 30 гигабаз данных ДНК
Автор прочитал свой геном прямо на кухонном столе. В статье — полный протокол без воды: какое железо купить, как не слить бюджет на проточную ячейку и как настроить таргетное обогащение, чтобы получить ответы именно по вашим вопросам. Переходите, если хотите понять, как устроена современная DIY-генетика и с чего начать свой первый прогон. Перейти к гайду
https://habr.com/ru/companies/flant/articles/1031594/
#секвенирование #геном #проточная_ячейка #minion #MinKNOW #лигирование #днк #спинколонка #Aтейлинг
-
[Перевод] Прочитал свой геном на кухне и превратил мазок щеки в 30 гигабаз данных ДНК
Автор прочитал свой геном прямо на кухонном столе. В статье — полный протокол без воды: какое железо купить, как не слить бюджет на проточную ячейку и как настроить таргетное обогащение, чтобы получить ответы именно по вашим вопросам. Переходите, если хотите понять, как устроена современная DIY-генетика и с чего начать свой первый прогон. Перейти к гайду
https://habr.com/ru/companies/flant/articles/1031594/
#секвенирование #геном #проточная_ячейка #minion #MinKNOW #лигирование #днк #спинколонка #Aтейлинг
-
[Перевод] Прочитал свой геном на кухне и превратил мазок щеки в 30 гигабаз данных ДНК
Автор прочитал свой геном прямо на кухонном столе. В статье — полный протокол без воды: какое железо купить, как не слить бюджет на проточную ячейку и как настроить таргетное обогащение, чтобы получить ответы именно по вашим вопросам. Переходите, если хотите понять, как устроена современная DIY-генетика и с чего начать свой первый прогон. Перейти к гайду
https://habr.com/ru/companies/flant/articles/1031594/
#секвенирование #геном #проточная_ячейка #minion #MinKNOW #лигирование #днк #спинколонка #Aтейлинг
-
@aaron.rupar It isn't easy to be a #Trump #Minion, you have to throw under the bus for him.
-
Installing a Minion (How Hard Can it be?) — super-clear OpenNMS walkthrough for setting up remote monitoring agents. Step-by-step, practical, and ideal for sysadmins & network engineers deploying at scale. Quick to follow, big payoff! #OpenNMS #Minion #Installation #Tutorial #Networking #SysAdmin #DevOps #English
https://media.no42.org/videos/watch/35d3ec73-d3a6-4558-98d6-2a2285cbc821 -
-
-
I am working on my first #Minion metabarcoding run. I've got a library of dual-indexed amplicons (ITS2). Ran it through the Minion, base called, and now I'm working on demultiplexing.
I have discovered that some of my reads contain multiple amplicons. i.e., a single line in the fastq file includes index1-primer-read1-primer-index2-index3-primer-read2-primer-index4
Is that a problem with my library, the sequencing process, or is it something I can fix at this stage (i.e., computationally)?
-
I am working on my first #Minion metabarcoding run. I've got a library of dual-indexed amplicons (ITS2). Ran it through the Minion, base called, and now I'm working on demultiplexing.
I have discovered that some of my reads contain multiple amplicons. i.e., a single line in the fastq file includes index1-primer-read1-primer-index2-index3-primer-read2-primer-index4
Is that a problem with my library, the sequencing process, or is it something I can fix at this stage (i.e., computationally)?
-
Respect to whoever went the extra mile to make this Minion letterbox.
I stopped to photograph it at the entrance to a rural driveway in Lyttelton Harbour. I like how yellow reflectors have been added to it can be seen by car traffic.
Some skilled parent must be very popular with their children. 😀
#letterbox #Minion #DespicableMe #GovernorsBay #LytteltonHarbour #NZ #Christchurch #MetalWork
-
Respect to whoever went the extra mile to make this Minion letterbox.
I stopped to photograph it at the entrance to a rural driveway in Lyttelton Harbour. I like how yellow reflectors have been added to it can be seen by car traffic.
Some skilled parent must be very popular with their children. 😀
#letterbox #Minion #DespicableMe #GovernorsBay #LytteltonHarbour #NZ #Christchurch #MetalWork
-
Oh I'm one of those, it's called operation often now??? This is Lunarbase Dagon facility shit... It's the Andromedan virus creation is fighting against... the virus vs. all creation. #viy #vey #lunarbase #hasidic #munchkin #minion #israeli #arachne #operationoften
https://www.facebook.com/share/r/1FAQPa8F96/ -
@nanopore DRS sequencing temperature was lowered to 30/31C (#P2 / #MinION) in #minknow v24.06 (and all recent versions) slowing down RNA helicase from 130 bps to 100-110 bps, lowering yield per hour and affecting signal readouts.
In effect, recent and older #RNA004 runs differ in base- and modification calling quality, possibly pA tail estimates.
This change was not widely announced to the community apart from a brief mention in software release notes!
https://community.nanoporetech.com/posts/software-release-24-06-for
#academicChatter -
@nanopore DRS sequencing temperature was lowered to 30/31C (#P2 / #MinION) in #minknow v24.06 (and all recent versions) slowing down RNA helicase from 130 bps to 100-110 bps, lowering yield per hour and affecting signal readouts.
In effect, recent and older #RNA004 runs differ in base- and modification calling quality, possibly pA tail estimates.
This change was not widely announced to the community apart from a brief mention in software release notes!
https://community.nanoporetech.com/posts/software-release-24-06-for
#academicChatter -
@nanopore DRS sequencing temperature was lowered to 30/31C (#P2 / #MinION) in #minknow v24.06 (and all recent versions) slowing down RNA helicase from 130 bps to 100-110 bps, lowering yield per hour and affecting signal readouts.
In effect, recent and older #RNA004 runs differ in base- and modification calling quality, possibly pA tail estimates.
This change was not widely announced to the community apart from a brief mention in software release notes!
https://community.nanoporetech.com/posts/software-release-24-06-for
#academicChatter -
@nanopore DRS sequencing temperature was lowered to 30/31C (#P2 / #MinION) in #minknow v24.06 (and all recent versions) slowing down RNA helicase from 130 bps to 100-110 bps, lowering yield per hour and affecting signal readouts.
In effect, recent and older #RNA004 runs differ in base- and modification calling quality, possibly pA tail estimates.
This change was not widely announced to the community apart from a brief mention in software release notes!
https://community.nanoporetech.com/posts/software-release-24-06-for
#academicChatter -
@nanopore DRS sequencing temperature was lowered to 30/31C (#P2 / #MinION) in #minknow v24.06 (and all recent versions) slowing down RNA helicase from 130 bps to 100-110 bps, lowering yield per hour and affecting signal readouts.
In effect, recent and older #RNA004 runs differ in base- and modification calling quality, possibly pA tail estimates.
This change was not widely announced to the community apart from a brief mention in software release notes!
https://community.nanoporetech.com/posts/software-release-24-06-for
#academicChatter -
A most excellent #minion #pool #birthdayCake 🤓
(made by very talented daughter 👍) -
Eu quando o aplicativo me bloqueia após eu mudar minha foto de perfil e nome de usuário: #medesbloqueia #porque #meme #minion #poxa
-
Eu quando o aplicativo me bloqueia após eu mudar minha foto de perfil e nome de usuário: #medesbloqueia #porque #meme #minion #poxa
-
Eu quando o aplicativo me bloqueia após eu mudar minha foto de perfil e nome de usuário: #medesbloqueia #porque #meme #minion #poxa
-
https://www.theverge.com/news/658829/elizabeth-warren-jeff-bezos-amazon-trump-tariffs
Elizabeth Warren asks Jeff Bezos if he got any favors for his ‘subservience’ to Trump
#warren #amazon #AMZN #Bezos #favors #trump #tariffs #customers #consumers #sycophant #fawner #flatterer #toadie #techbros #oligarchy #oligarch #obsequious #minion #kowtow #bootlicker #brownnoser #kissup #suckup #sellout #retail #shopping #consumer #spending #kakistocracy
-
Australia's Prime #Minion, Anthony Albanese (aka #Albo) 2023 historic $368 billion deal with #AUKUS for eight USA nuclear-powered submarines in “three decade” … hard to swallow crunchy sub.
https://streetartcities.com/markers/69248109-d10e-44ee-9f91-3bf9921c8699
#AUKUS #AusPol #MilitaryIndustrialComplex #NuclearSubmarines #SubWay
#art #illustration #Mural #VanNishing #VanTRudd -
Australia's Prime #Minion, Anthony Albanese (aka #Albo) 2023 historic $368 billion deal with #AUKUS for eight USA nuclear-powered submarines in “three decade” … hard to swallow crunchy sub.
https://streetartcities.com/markers/69248109-d10e-44ee-9f91-3bf9921c8699
#AUKUS #AusPol #MilitaryIndustrialComplex #NuclearSubmarines #SubWay
#art #illustration #Mural #VanNishing #VanTRudd -
I have accepted the fact that that I don't have profiles on #spotify that it is broken as hell, but now that I see my #letterboxd also has "most watched actors" filled with people I did not know were the voices of the #Minion movies, I really need to push them to add a feature that makes it easy to separate "watched with kid" vs "watched with grown ups" 😅🤣
-
I have accepted the fact that that I don't have profiles on #spotify that it is broken as hell, but now that I see my #letterboxd also has "most watched actors" filled with people I did not know were the voices of the #Minion movies, I really need to push them to add a feature that makes it easy to separate "watched with kid" vs "watched with grown ups" 😅🤣
-
Meine #Minion -Lampe leuchtet jetzt am Fenster.
Gibt bestimmt missbilligende Blicke von der Weihnachtsbeleuchtungsmafia der Vororte :-D -
🏴☠️ Dank unseres 1. Vorsitzenden in #Niedersachsen bin ich im Besitz einer #Minion-#Piraten-Tasse … und ich benutze sie!
(englisch) »Walk The Plank« = (deutsch) „über die Planke gehen“
