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#minion — Public Fediverse posts

Live and recent posts from across the Fediverse tagged #minion, aggregated by home.social.

  1. Due to lack of popular demand, I've removed the dependency on "fish shell" from my #commandLine #journal tools.

    If interested, check out
    codeberg.org/EdTheDev/minion

    Now anyone using Bash shell can just clone #minion and add it to the path and start writing and searching for notes.

    If you're curious, contrast it with my previous version written in #Python, and then #PowerShell and #goLang.

    github.com/edthedev/minion

    As I've gained expeirence, I can accomplish roughly the same outcomes with less lines of code and fewer dependencies.

  2. Due to lack of popular demand, I've removed the dependency on "fish shell" from my #commandLine #journal tools.

    If interested, check out
    codeberg.org/EdTheDev/minion

    Now anyone using Bash shell can just clone #minion and add it to the path and start writing and searching for notes.

    If you're curious, contrast it with my previous version written in #Python, and then #PowerShell and #goLang.

    github.com/edthedev/minion

    As I've gained expeirence, I can accomplish roughly the same outcomes with less lines of code and fewer dependencies.

  3. [Перевод] Прочитал свой геном на кухне и превратил мазок щеки в 30 гигабаз данных ДНК

    Автор прочитал свой геном прямо на кухонном столе. В статье — полный протокол без воды: какое железо купить, как не слить бюджет на проточную ячейку и как настроить таргетное обогащение, чтобы получить ответы именно по вашим вопросам. Переходите, если хотите понять, как устроена современная DIY-генетика и с чего начать свой первый прогон. Перейти к гайду

    habr.com/ru/companies/flant/ar

    #секвенирование #геном #проточная_ячейка #minion #MinKNOW #лигирование #днк #спинколонка #Aтейлинг

  4. @aaron.rupar It isn't easy to be a #Trump #Minion, you have to throw under the bus for him.

  5. Someone has made creative use of the snow in Ueno Park!

    #tokyo #ueno #japan #minion

  6. Someone has made creative use of the snow in Ueno Park!

    #tokyo #ueno #japan #minion

  7. #Bioinformatics Q:

    I am working on my first #Minion metabarcoding run. I've got a library of dual-indexed amplicons (ITS2). Ran it through the Minion, base called, and now I'm working on demultiplexing.

    I have discovered that some of my reads contain multiple amplicons. i.e., a single line in the fastq file includes index1-primer-read1-primer-index2-index3-primer-read2-primer-index4

    Is that a problem with my library, the sequencing process, or is it something I can fix at this stage (i.e., computationally)?

  8. #Bioinformatics Q:

    I am working on my first #Minion metabarcoding run. I've got a library of dual-indexed amplicons (ITS2). Ran it through the Minion, base called, and now I'm working on demultiplexing.

    I have discovered that some of my reads contain multiple amplicons. i.e., a single line in the fastq file includes index1-primer-read1-primer-index2-index3-primer-read2-primer-index4

    Is that a problem with my library, the sequencing process, or is it something I can fix at this stage (i.e., computationally)?

  9. Respect to whoever went the extra mile to make this Minion letterbox.

    I stopped to photograph it at the entrance to a rural driveway in Lyttelton Harbour. I like how yellow reflectors have been added to it can be seen by car traffic.

    Some skilled parent must be very popular with their children. 😀

    #letterbox #Minion #DespicableMe #GovernorsBay #LytteltonHarbour #NZ #Christchurch #MetalWork

  10. Respect to whoever went the extra mile to make this Minion letterbox.

    I stopped to photograph it at the entrance to a rural driveway in Lyttelton Harbour. I like how yellow reflectors have been added to it can be seen by car traffic.

    Some skilled parent must be very popular with their children. 😀

    #letterbox #Minion #DespicableMe #GovernorsBay #LytteltonHarbour #NZ #Christchurch #MetalWork

  11. Oh I'm one of those, it's called operation often now??? This is Lunarbase Dagon facility shit... It's the Andromedan virus creation is fighting against... the virus vs. all creation. #viy #vey #lunarbase #hasidic #munchkin #minion #israeli #arachne #operationoften
    facebook.com/share/r/1FAQPa8F9

  12. @nanopore DRS sequencing temperature was lowered to 30/31C (#P2 / #MinION) in #minknow v24.06 (and all recent versions) slowing down RNA helicase from 130 bps to 100-110 bps, lowering yield per hour and affecting signal readouts.
    In effect, recent and older #RNA004 runs differ in base- and modification calling quality, possibly pA tail estimates.
    This change was not widely announced to the community apart from a brief mention in software release notes!
    community.nanoporetech.com/pos
    #academicChatter

  13. @nanopore DRS sequencing temperature was lowered to 30/31C (#P2 / #MinION) in #minknow v24.06 (and all recent versions) slowing down RNA helicase from 130 bps to 100-110 bps, lowering yield per hour and affecting signal readouts.
    In effect, recent and older #RNA004 runs differ in base- and modification calling quality, possibly pA tail estimates.
    This change was not widely announced to the community apart from a brief mention in software release notes!
    community.nanoporetech.com/pos
    #academicChatter

  14. Eu quando o aplicativo me bloqueia após eu mudar minha foto de perfil e nome de usuário: #medesbloqueia #porque #meme #minion #poxa

  15. Eu quando o aplicativo me bloqueia após eu mudar minha foto de perfil e nome de usuário: #medesbloqueia #porque #meme #minion #poxa

  16. Eu quando o aplicativo me bloqueia após eu mudar minha foto de perfil e nome de usuário: #medesbloqueia #porque #meme #minion #poxa

  17. I have accepted the fact that that I don't have profiles on #spotify that it is broken as hell, but now that I see my #letterboxd also has "most watched actors" filled with people I did not know were the voices of the #Minion movies, I really need to push them to add a feature that makes it easy to separate "watched with kid" vs "watched with grown ups" 😅🤣

  18. I have accepted the fact that that I don't have profiles on #spotify that it is broken as hell, but now that I see my #letterboxd also has "most watched actors" filled with people I did not know were the voices of the #Minion movies, I really need to push them to add a feature that makes it easy to separate "watched with kid" vs "watched with grown ups" 😅🤣

  19. Meine #Minion -Lampe leuchtet jetzt am Fenster.
    Gibt bestimmt missbilligende Blicke von der Weihnachtsbeleuchtungsmafia der Vororte :-D

  20. 🏴‍☠️ Dank unseres 1. Vorsitzenden in #Niedersachsen bin ich im Besitz einer #Minion-#Piraten-Tasse … und ich benutze sie!
    (englisch) »Walk The Plank« = (deutsch) „über die Planke gehen“

  21. 🏴‍☠️ Dank unseres 1. Vorsitzenden in #Niedersachsen bin ich im Besitz einer #Minion-#Piraten-Tasse … und ich benutze sie!
    (englisch) »Walk The Plank« = (deutsch) „über die Planke gehen“

  22. My weekly newsletter’s out! We’re talking:
    - The totally normal #Minion #Jesus meme and the #SevenMountain mandate
    - The #Refugee #Paralympics Team medals for the first time
    - #Trump accidentally offends #White #Christian nationalists at #Arlington
    - Librarians fighting the war on despair
    - And @Jonathanpanwalton keeps us grounded with a healing sermon
    Check it out and subscribe for free! ktfpress.us/9-5-24News #KTFPress #Christianity #Faith #Military #Disability #libraries

  23. My weekly newsletter’s out! We’re talking:
    - The totally normal #Minion #Jesus meme and the #SevenMountain mandate
    - The #Refugee #Paralympics Team medals for the first time
    - #Trump accidentally offends #White #Christian nationalists at #Arlington
    - Librarians fighting the war on despair
    - And @Jonathanpanwalton keeps us grounded with a healing sermon
    Check it out and subscribe for free! ktfpress.us/9-5-24News #KTFPress #Christianity #Faith #Military #Disability #libraries

  24. What's that you say? You want an undergraduate practical course that teaches #MinION sequencing of the skin microbiome? Here you go:

    biorxiv.org/content/10.1101/20

    #Biology #Hull #microbiome #UKHE