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#thatsecondreviewer — Public Fediverse posts

Live and recent posts from across the Fediverse tagged #thatsecondreviewer, aggregated by home.social.

  1. Nachiket Vartak @[email protected] ·

    We just put in a manuscript using #siRNA for knockdown. Knockdown verified with RT-PCR and Western blot. Saw a phenotype. Rescued it with ectopic expression of the knocked down protein. A reviewer required us to repeat the experiments using #CRISPR, and why we didn''t use crispr in the first place ?#thatsecondreviewer
    #academia #cellbiology
    A: Because we didn't need to, <expletive>.

    #cellbiology #academia #thatsecondreviewer #crispr #sirna
  2. Nachiket Vartak @[email protected] ·

    We just put in a manuscript using #siRNA for knockdown. Knockdown verified with RT-PCR and Western blot. Saw a phenotype. Rescued it with ectopic expression of the knocked down protein. A reviewer required us to repeat the experiments using #CRISPR, and why we didn''t use crispr in the first place ?#thatsecondreviewer
    #academia #cellbiology
    A: Because we didn't need to, <expletive>.

    #cellbiology #academia #thatsecondreviewer #crispr #sirna