#bioorthogonal — Public Fediverse posts

🚨New manuscript: interorganelle lipid transport is rapid, & it is hard to quantify discrete steps in this network, mediated by lipid transfer proteins working at membrane contact sites between different organelles. We (Dongjun Liang & Lin Luan) harness chemoenzymatic tagging & super-fast #bioorthogonal chemistry to make fluorescent lipids that report on PM-to-ER phospholipid flux mediated by a family of lipid transfer proteins at these contact sites! https://doi.org/10.1101/2023.02.01.526230 #lipidtime #chembio

@bertozzi #glycotime #bioorthogonal #chemnobel #chemnobelles

Thrilled to share this story in JACS Au! https://pubs.acs.org/doi/10.1021/jacsau.2c00419

Takeaways:
1) analogs of PC lipids w/different choline structures adopt different organelle localizations & are useful for tracing interorganelle lipid transport
2) OCT1, a liver transporter typically used to study PK/drug efflux, can also facilitate uptake of small cationic #ChemicalBiology probes, including #bioorthogonal choline analogs. Available soon @Addgene https://addgene.org/188995/

Congrats, Dylan!
#lipidtime #OpenAccess

Hi everyone! I'm @basktastic on Twitter but am trying out my real name here. My #chembio #cellbio #lipidtime lab at Cornell develops tools for imaging and editing the lipidome, drawing on #bioorthogonal chemistry, #clickchemistry, #chemoproteomics, #optogenetics, #directedevolution, and more. https://baskin.wicmb.cornell.edu