#zp2 — Public Fediverse posts

In this work we use #crystallography and #cryoem to study egg zona pellucida (#ZP) glycoprotein #ZP2, in both its intact (pre-fertilization) and cleaved (post-fertilization) state.

We also describe the first structure of a heteromeric vertebrate egg coat filament.

New discovery reveals how the #egg controls #sperm entry.

#fertilization #egg_coat #ZP2

https://phys.org/news/2024-03-discovery-reveals-egg-sperm-entry.html

The 4 subunits that make up an oocyte's zona peculida. Something seems to be up with the mRNA atlas entries for ZP3:r.

#ZP1, #ZP2, #ZP3, #ZP4 (zona pellucida glycoprotein 1, 2, 3 & 4): Human Protein Atlas Concensus mRNA tissue distributions & GPMDB protein tissue tabbulations. #proteomics

In this work, we combine #crystallography, #cryoEM, #AlphaFold predictions, biochemical and mouse studies to unveil the 3D structure of vertebrate egg coat filaments and describe how cleavage of their #ZP2 subunit hardens the coat, establishing a permanent block to #polyspermy.

A very big thank you to all co-authors, collaborators, and funding bodies for making this possible!

After a chase that lasted more than 10 years, finally here it is:

Architecture of the Vertebrate Egg Coat and Structural Basis of the ZP2 Block to Polyspermy
https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4470110

#fertilization #oocyte #sperm #zonapellucida #zp_domain #zp_module #zp1 #zp2 #zp3 #infertility