#sted — Public Fediverse posts

Hier die Signature-Grafik aus der Publikation.

#STED #Röntgen #XRay https://www.nature.com/articles/s41467-018-05885-z

Für einige Experimente haben wir ein #STED-Mikroskop zur superauflösenden #Fluoreszenz-#Mikroskopie in unser Röntgenmikroskop eingebaut, eine komplizierte Sonderanfertigung.

Hier eine sehr symbolhafte Darstellung, wie das Mikroskop funktioniert (siehe auch Alt-Text).

Nano-Scale Insights into Synapses: Transforming Our Understanding of Psychiatric Disorders

#Neuroscience #SynapticNanoarchitecture #SuperResolutionMicroscopy #MolecularNeuroscience #BrainResearch #MentalHealth #Nanotechnology #DNA-PAINT #STED #smFRET #Synapse #Neurotech #Psychiatry #BrainScience #PrecisionMedicine

https://youtube.com/shorts/N9RORGpcWPY?feature=share

#ScientificPic 🖼️🧪Tiffany Cloâtre has produced magnificent rat neurons imaged using #STED technology🔬

Thanks to The Bordeaux Imaging Center!

@cnrs
@univbordeaux

#Janelia is offering a 12-day bootcamp designed to demonstrate how biological queries and hypotheses steer experimental designs on various #microscopy platforms and across length scales from molecules to small animals:

⏰ J uly 28 - August 9, 2024
⏰ Deadline to apply: Feb 15 (11:59 p.m. ET)
🌍 https://www.janelia.org/you-janelia/conferences/bootcamp-course-optical-imaging-across-biological-length-scales?Email_Preferences_Subscription_Center=&mc_cid=a9a3274574&mc_eid=fb2da94283

#SuperResolutionMicroscopy #STED #LightSheetMicroscopy #ImageProcessing #DataAnalysis #FOSS #BigData

Now open for registrations: short course on single molecule techniques in molecular #biophysics, as part of the #MOSBRI network. This course is hosted at the University of #Groningen by our colleagues of the molecular biophysics group. NOTE deadline: Sept 15th for registration! Course itself is in early November: https://www.mosbri.eu/training/end-user-short-courses/esc7/ Topics: #microscopy #AFM #superresolution #STED

Image obtained with the @LeicaMicro SP8 STED
---
RT @louvel_vincent
First time doing #STED microscopy on Nuclear Pore Complexes with NUP96 staining, absolutely stunting 😵‍💫
https://twitter.com/louvel_vincent/status/1649061277895995394

🔶 What are the additional insights you can gain from imaging tissue sample in 10 colors?
🔶 Follow our on-demand webinar with Dr. Nicolas Gaudenzio and discover how to perform 10-color imaging using a confocal microscope. See how this allows to assess the skin immune landscape.

https://fcld.ly/lciceh8

#STED #STELLARIS #confocal #immune

Tim Salditt (http://twitter.com/SaldittLab, substituting Jasper Frohn): #Multiscale #Xray Phase Contrast #Tomography at #GINIX/P10: Concepts, Implementation and Applications

- fantastic collaboration with the P10 team
- directly comparing #STED to #Minflux; old #confocal? not worth mentioning 😂
- overview tomo, then zoom-in
- tumorous human pancreatic tissue #biopsy: quantify the #tumor type
- from electron density to metrics, quantify fibres etc.
- #hippocampus patho punch, #Alzheimer sample

Are you unfamiliar with the terms #STED, #PALM, #STORM, #SMLM, #MINFLUX, & #MINSTED, but would you like to learn more about these #superresolution #microscopy technologies🔬? If so, then you should check out #StefanHell's #Lindau presentation 🤓 Enjoy👉 https://mediatheque.lindau-nobel.org/recordings/39775

I came across this #microscopy paper. https://www.nature.com/articles/s41587-022-01519-4
"By blue-shifting the #STED beam and separating #fluorophores by on/off switching, individual fluorophores bound to a #DNA strand are localized with σ = 4.7 Å, corresponding to a fraction of the fluorophore size, with only 2,000 detected #photons ."
This image showing resolution below the size of the fluorophore leaves me speechless.
The paper features images of #DNAorigami, #NuclearPores, synaptic #Vesicles, #ECM (#laminin) and #vilmentin

Hi #science mastodon! My work boils down to this question: Is membrane-proximal #actin generating #diffusion barriers for #membrane molecules?

I am a PhD student of Helge Ewers at FU Berlin. I work in #neurons because actin forms very well-behaved rings there. My approach is combining #SingleParticleTracking of membrane proteins and #SuperResolution #microscopy of the #cytoskeleton via #STORM and #STED. Lots of #biophysics , #CellBiology and #neuroscience. #introduction #ScienceMastodon