#ssnmr — Public Fediverse posts

Mark the date! The next Alpine conference on magnetic resonance in solids is planned for Sept 14-18 2025, as usual in beautiful #Chamonix-Mont-Blanc in the Alps. The invited speakers are listed on the conference website already: https://alpine-conference.org The registration opens early next year. #NMRchat #ssNMR

Slightly delayed due to my holidays, but excited to share this new paper that came out online a little while ago in ACS Applied Materials and Interfaces: https://doi.org/10.1021/acsami.4c08428 In it, PhD student Pushpa Rampratap spearheaded our effort to use MAS #NMR to study ECM-like #hydrogels. In a prior publication (https://doi.org/10.1016/j.carbpol.2023.121063) she described methods for preparing 13C enriched version of the polysaccharide hyaluronic acid…. #glycotime #nmrchat #ssnmr

Our latest (review) article is now available online: https://doi.org/10.1042/BST20230731 in the journal Biochemical Transactions. In this invited article “Solid-state nuclear magnetic resonance in the structural study of polyglutamine aggregation”, I have reviewed the contributions that solid-state #NMR #SSNMR has made to our understanding of the mutant protein aggregates in #huntingtonsdisease and related #polyQ disorders. (1/2)

Among many other topics, you can find discussion of how #SSNMR is particularly useful to understand the surface features of protein aggregates (their fuzzy coat), which are often hard to see by cryo-EM and other techniques. (2/2)

Photo from yesterday’s official opening of the new CryoMAS #ssNMR platform in #umea (Sweden). Great talks covering all aspects of #ssnmr and its diverse applications.

Call for papers! Solid-State NMR and DNP of Heterogeneous Biomaterials and Cellular Systems https://www.sciencedirect.com/journal/solid-state-nuclear-magnetic-resonance/about/call-for-papers#solid-state-nmr-and-dnp-of-heterogeneous-biomaterials-and-cellular-systems -… #NMRchat #SSNMR

Busy week coming - but am definitely looking forward to next week visiting #Cambridge (UK) to discuss #amyloid #ssNMR #ECM #polysaccharides and more. Actually my first visit there, although I spent quite a few years in the 'other' Cambridge (MASS/USA). :-)

Chamonix hike during the #alpineMR2023 conference #ssNMR #nmrchat

Don’t miss the #iupacchains2023 parallel session 10 on Structural Biology and Protein Design at 10:20am - see the talk by my PhD student Raffaella Parlato! She will talk about our work with Wiktor Szymanski’s team (also University of #Groningen) - enabling #photochemical control over #polyQ #amyloid formation & structure. #ssNMR #chemistry

… using in-depth #moleculardynamics simulation to integrate various experimental constraint provides an unprecedented view of the structure of the misfolded protein aggregates, with a central role (of course) for new and existing #ssNMR data. These #polyQ protein fibrils are tough for #cryoEM due to structural and dynamic heterogeneity.
….

... forgot the screenshot of their modeling the air flows in their 3D printed stator design. #nmrchat #ssnmr

Cool new paper from Joern Schmedt auf der Guenne and coworkers, in the journal #SSNMR: “High resolution solid-state #NMR on the desktop”, presenting a conical MAS rotor design for use in permanent magnets. Looks cool! The team includes Oliver Pecher and Marco Braun from the NMR-service company, which is doing various other interesting things when it comes to #ssNMR hardware! https://www.sciencedirect.com/science/article/pii/S0926204023000346

Well, just got back from the FASEB protein aggregation conference #PACSRC in Ireland. As usual, it was a great event with a never-ending stream of cool science. Quite a few #amyloid structures discusses, based on #ssNMR and #cryoEM . I briefly talked about our own integrative structural biology work on the fibrils made by mutant huntingtin fragments #huntingtonsdisease , as well as our work with aggregation inhibitors (and how they reveal features of the aggregation mechanism) #polyQ

Abstracts for the alpine conference on magnetic resonance in solids are almost due!! Getting into the mood.. #nmrchat #ssnmr

Coming on May 30th: online webinar by Dr. Kaustubh Mote from the Tata Institute of Fundamental Research (TIFR) #hyderabad on the topic of “Measuring dynamics using anisotropic interactions in MAS-NMR”. More info: https://www.globalnmr.org/upcoming-talks Register to attend here: https://www.globalnmr.org/weekly-registration

#NMR #nmrchat #ssNMR #dipoles #dynamics #biophysics

Nuclear spin diffusion under fast magic-angle spinning in solid-state #nmr by Lewandowski and colleagues, in the journal of chemical physics: https://pubs.aip.org/aip/jcp/article/158/18/184201/2890210/Nuclear-spin-diffusion-under-fast-magic-angle #nmrchat #ssNMR

New Diamond Rotors for #ssNMR. By Griffin and colleagues. #nmrchat https://www.sciencedirect.com/science/article/abs/pii/S1090780723001106

New collaborative article now available online: "A Universal Nanogel‐Based Coating Approach for Medical Implant Materials" in the journal Advanced NanoBiomed Research. With the group of Patrick van Rijn #BME #UMCG and others. See: https://onlinelibrary.wiley.com/doi/10.1002/anbr.202200141 We contributed some #ssNMR analysis of the nanogel to probe its composition and modification. #nmrchat #nanomaterials

A new preprint from the lab (together with Marthe Walvoort's group) is now on #bioRxiv : https://www.biorxiv.org/content/10.1101/2023.03.16.532902v1 This manuscript presents nice work by Pushpa Rampratap, who did a great job figuring out how to make 13C-enriched hyaluronic acid #polysaccharide for use in #ssNMR . More forthcoming on the applications, but this paper introduces the protocols for producing the high-molecular weight HA. Enjoy! #glycotime #nmrchat #TeamMassSpec (Feedback welcome!)

Coming up: webinar in the Global #NMR discussion forum, by Leonard Mueller: "NMR crystallography: integrative foundations and applications to materials chemistry and structural biology" Happening on March 21st! See here: https://www.globalnmr.org/upcoming-talks #ssNMR #nmrchat

The #ssNMR analysis was used (among several other techniques) to probe the time-dependent changes in chemical structure during the polymerization process.

A new paper by our colleagues from the Zernike Institute (U. Groningen) has just been published, including some #ssNMR from my group. The paper looks at the process of polydopamine formation the presence and absence of halloysite #nanotubes. See the paper here: https://doi.org/10.1038/s41467-023-36303-8 #polymers

Coming on Thu Feb 2nd: a special online workshop on handling and studying of fibrillar and #amyloid proteins. With presentations by Per Hammarström (Linköping, Sweden), Beat Meier (ETH, Switzerland), Stefano Ricagno (Milan, Italy), Byron Caughey (NIH, USA), and myself (Groningen). Topics: #prion propagation & seeding, structural studies by #ssNMR and #cryoEM, toxicity and biosafety. See #biophysics MOSBRI website: https://www.mosbri.eu/events/workshop-handling-fibrillar-and-amyloid-proteins/

The MOSBRI network (https://www.mosbri.eu ) is a Europe-wide molecular-scale #biophysics research infrastructure network. Biophysics groups at our university (#Groningen) participate with capabilities in single molecule studies, AFM, and #ssNMR: https://www.mosbri.eu/partners/rug-bp/ . Our network offers funds for trans-national access (TNA). So please reach out if you are curious about trying #ssNMR studies of your e.g. protein aggregates, #amyloid or membrane-bound proteins.

New #ssNMR paper in JACS: "A Complete Picture of Cation Dynamics in Hybrid #Perovskite Materials from Solid-State NMR Spectroscopy" by Emsley and colleagues: 2H and 14N quadrupolar solid-state NMR relaxometry under magic-angle spinning https://pubs.acs.org/doi/full/10.1021/jacs.2c10149 #nmrchat

Nice new review article on "Ultrafast Magic Angle Spinning Solid-State NMR Spectroscopy: Advances in Methodology and Applications" by Nishiyama, Hou, Su, & Ramamoorthy - ACS journal Chem. Rev: https://pubs.acs.org/doi/abs/10.1021/acs.chemrev.2c00197 #nmrchat #ssNMR

@AnsgarSiemer It is a pity that these #ssNMR experiments do not distinguish different #tau #amyloid conformations, such as the #AD and #AGD folds. Would #NOE distance measurements help, or is that not possible for these samples?

(It is in part a follow-up on our earlier collaborative paper, more focused on the #DNP #SSNMR analysis of unlabeled #polyQ #protein #amyloids - https://pubs.acs.org/doi/10.1021/jacs.8b09002 )

Our department at the Zernike Institute for Advanced Materials of the University of #Groningen is highlighting Raffaella Parlato from my #SSNMR group in this year's advent calendar: Raffaella's research focuses on #HuntingtonsDisease, a rare and neurodegenerative disease caused by #polyQ #protein aggregation into #amyloid fibrils . Read more: https://www.rug.nl/research/zernike/news/newsitems/20221220-day20-parlato