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#replicates — Public Fediverse posts

Live and recent posts from across the Fediverse tagged #replicates, aggregated by home.social.

  1. Just reading this very interesting paper on how many #replicates are needed for appropriate analysis of bulk #RNAseq data and comparing tools for DE gene expression.

    #bioinformatics #article #goodread

    ncbi.nlm.nih.gov/pmc/articles/

    From the abstract

    With three biological replicates, nine of the 11 tools evaluated
    found only 20%–40% of the significantly differentially expressed (SDE) genes identified with the full set of 42 clean replicates.
    This rises to >85% for the subset of SDE genes changing in expression by more than fourfold. To achieve >85% for all SDE genes regardless of fold change requires more than 20 biological replicates.