#qpcr — Public Fediverse posts

Master these essential biotech lab techniques every student must know! From DNA extraction & PCR to Western Blotting—level up your skills with BioResire. Join our WhatsApp channel for free guides: https://whatsapp.com/channel/0029Vb5u2Tp77qVTb1D7bu3r

#BiotechTechniques #DNACultivation #RNAIsolation #AgaroseGel #PCRTechniques #qPCR #GelDocumentation #RestrictionEnzyme #LigationTransformation #PlasmidIsolation #SiteDirectedMutagenesis #ProteinPurification #SDSPAGE #PolyacrylamideGel #Electrophoresis #WesternBlotting

Master these essential biotech lab techniques every student must know! From DNA extraction & PCR to Western Blotting—level up your skills with BioResire. Join our WhatsApp channel for free guides: https://whatsapp.com/channel/0029Vb5u2Tp77qVTb1D7bu3r

#BiotechTechniques #DNACultivation #RNAIsolation #AgaroseGel #PCRTechniques #qPCR #GelDocumentation #RestrictionEnzyme #LigationTransformation #PlasmidIsolation #SiteDirectedMutagenesis #ProteinPurification #SDSPAGE #PolyacrylamideGel #Electrophoresis #WesternBlotting

Every biotech student must master these essential lab techniques!
From DNA extraction & PCR to Western blotting at BioResire.
Learn hands-on: [email protected]
#Biotechnology #Biotech #MolecularBiology #PCR #DNAextraction #qPCR #GelElectrophoresis #WesternBlot #RNAPurification #RestrictionDigestion #PlasmidIsolation #SiteDirectedMutagenesis #SDSPAGE #BiotechStudent #LifeSciences #BioResire #BiotechTechniques #LabSkills #Biochemistry #Microbiology

Every biotech student must master these essential lab techniques!
From DNA extraction & PCR to Western blotting at BioResire.
Learn hands-on: [email protected]
#Biotechnology #Biotech #MolecularBiology #PCR #DNAextraction #qPCR #GelElectrophoresis #WesternBlot #RNAPurification #RestrictionDigestion #PlasmidIsolation #SiteDirectedMutagenesis #SDSPAGE #BiotechStudent #LifeSciences #BioResire #BiotechTechniques #LabSkills #Biochemistry #Microbiology

Every biotech student must master these essential lab techniques!
From DNA extraction & PCR to Western blotting at BioResire.
Learn hands-on: [email protected]
#Biotechnology #Biotech #MolecularBiology #PCR #DNAextraction #qPCR #GelElectrophoresis #WesternBlot #RNAPurification #RestrictionDigestion #PlasmidIsolation #SiteDirectedMutagenesis #SDSPAGE #BiotechStudent #LifeSciences #BioResire #BiotechTechniques #LabSkills #Biochemistry #Microbiology

Every biotech student must master these essential lab techniques!
From DNA extraction & PCR to Western blotting at BioResire.
Learn hands-on: [email protected]
#Biotechnology #Biotech #MolecularBiology #PCR #DNAextraction #qPCR #GelElectrophoresis #WesternBlot #RNAPurification #RestrictionDigestion #PlasmidIsolation #SiteDirectedMutagenesis #SDSPAGE #BiotechStudent #LifeSciences #BioResire #BiotechTechniques #LabSkills #Biochemistry #Microbiology

Every biotech student must master these essential lab techniques!
From DNA extraction & PCR to Western blotting at BioResire.
Learn hands-on: [email protected]
#Biotechnology #Biotech #MolecularBiology #PCR #DNAextraction #qPCR #GelElectrophoresis #WesternBlot #RNAPurification #RestrictionDigestion #PlasmidIsolation #SiteDirectedMutagenesis #SDSPAGE #BiotechStudent #LifeSciences #BioResire #BiotechTechniques #LabSkills #Biochemistry #Microbiology

Evaluating #primer and #probe #mismatch tolerance in an #Influenza A #matrix gene RT #qPCR using contemporary human and zoonotic strains, https://etidiohnew.blogspot.com/2026/02/evaluating-primer-and-probe-mismatch.html

#pcr #qpcr #rtqpcr #academia

Anyone know of a good way to examine EDS files from a Thermofisher RT-PCR machine that doesn't require installing the software suite?

I can probably get the install file. But I fundamentally oppose data locked into a proprietary format for long-term archiving.

Following #rstats packages were maintained and are back on #CRAN now thanks to Andrej Spiess

- #dpcR, 2025-06-18, Digital PCR Analysis <10.32614/CRAN.package.dpcR>
- #MBmca, 2025-06-11, Nucleic Acid Melting Curve Analysis (https://journal.r-project.org/articles/RJ-2013-024/)
- #qpcR, 2025-06-10, Modelling and Analysis of Real-Time PCR Data <doi10.1093/bioinformatics/btn227>
- #PCRedux, 2025-06-13, Quantitative #PCR (#qPCR) Data Mining and Machine Learning Toolkit as Described in <doi:10.21105/Joss.04407>

Europe Digital PCR Industry Research Report 2025: Increased https://www.byteseu.com/1046289/ #DigitalPcr #DigitalPCRDPCR #Dpcr #Europe #MolecularDiagnostics #NextGenerationSequencing #NextGenerationSequencingNGS #NGS #PCR #Qpcr #ResearchAndMarkets

Our paper "MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines" is now published in Clinical Chemistry! 📚🔬

This update addresses recent advances in qPCR technology, providing clear recommendations on sample handling, assay design, and data analysis. We emphasize transparency and reproducibility to enhance the reliability of qPCR research.

https://doi.org/10.1093/clinchem/hvaf043 #MIQE2_0 #MIQE #qPCR #ResearchIntegrity #ScientificMethodology 📊🔍

For the next few months, Dr. Andrej-Nikolai Spiess (https://openalex.org/works?page=1&filter=authorships.author.id%3Aa5027948408&sort=publication_year%3Adesc) will be a guest in my working group.

We are working on a paper where we show that 29 % of papers in top journals like Science, Nature & PNAS were skewed by a single influential data point! Time to rethink our reliance on p-values and explore alternative measures like #dfstat. #reproducibilitycrisis #linearregression #rstats

Moreover, we will work on #qPCR related software like PCRedux (https://joss.theoj.org/papers/10.21105/joss.04407)

#JOSS

For the next few months, Dr. Andrej-Nikolai Spiess (https://openalex.org/works?page=1&filter=authorships.author.id%3Aa5027948408&sort=publication_year%3Adesc) will be a guest in my working group.

We are working on a paper where we show that 29 % of papers in top journals like Science, Nature & PNAS were skewed by a single influential data point! Time to rethink our reliance on p-values and explore alternative measures like #dfstat. #reproducibilitycrisis #linearregression #rstats

Moreover, we will work on #qPCR related software like PCRedux (https://joss.theoj.org/papers/10.21105/joss.04407)

#JOSS

For the next few months, Dr. Andrej-Nikolai Spiess (https://openalex.org/works?page=1&filter=authorships.author.id%3Aa5027948408&sort=publication_year%3Adesc) will be a guest in my working group.

We are working on a paper where we show that 29 % of papers in top journals like Science, Nature & PNAS were skewed by a single influential data point! Time to rethink our reliance on p-values and explore alternative measures like #dfstat. #reproducibilitycrisis #linearregression #rstats

Moreover, we will work on #qPCR related software like PCRedux (https://joss.theoj.org/papers/10.21105/joss.04407)

#JOSS

For the next few months, Dr. Andrej-Nikolai Spiess (https://openalex.org/works?page=1&filter=authorships.author.id%3Aa5027948408&sort=publication_year%3Adesc) will be a guest in my working group.

We are working on a paper where we show that 29 % of papers in top journals like Science, Nature & PNAS were skewed by a single influential data point! Time to rethink our reliance on p-values and explore alternative measures like #dfstat. #reproducibilitycrisis #linearregression #rstats

Moreover, we will work on #qPCR related software like PCRedux (https://joss.theoj.org/papers/10.21105/joss.04407)

#JOSS

For the next few months, Dr. Andrej-Nikolai Spiess (https://openalex.org/works?page=1&filter=authorships.author.id%3Aa5027948408&sort=publication_year%3Adesc) will be a guest in my working group.

We are working on a paper where we show that 29 % of papers in top journals like Science, Nature & PNAS were skewed by a single influential data point! Time to rethink our reliance on p-values and explore alternative measures like #dfstat. #reproducibilitycrisis #linearregression #rstats

Moreover, we will work on #qPCR related software like PCRedux (https://joss.theoj.org/papers/10.21105/joss.04407)

#JOSS

Most problems have been fixed in the #PCRedux package. There are still issues with the #rgl package and #Matrix is causing trouble on older platforms (especially #Ubuntu) 🤔. We're still figuring out how to solve the rgl problem, unfortunately it depends on the #qpcR package which calculates some of our key parameters 💡.
More work ahead of us.

#rstats

New publication: Risk assessment of toxic #cyanobacterial blooms in recreational #waters: A comparative study of monitoring #methods. #microsystin #fluorometry #microscopy #qPCR
https://doi.org/10.1016/j.hal.2024.102683

#US #FDA, Updates on Highly Pathogenic Avian #Influenza #H5N1 (HPAI), as of April 23 2024, https://www.fda.gov/food/alerts-advisories-safety-information/updates-highly-pathogenic-avian-influenza-hpai

Based on available information, #pasteurization is likely to inactivate the virus, however the process is not expected to remove the presence of viral #particles. Therefore, some of the samples collected have indicated the presence of HPAI using quantitative polymerase chain reaction (#qPCR) testing.

#OTTO: #OpenSource affordable #liquid handler for #automated #micropipetting:

-applied to generate reproducible #qPCR data
-#Arduino-/#Python-controlled

#OpenAccess article: https://doi.org/10.1038/s41598-020-70465-5
Web: https://openliquidhandler.com/

#DIYbio #lab #instruments #automation #pipetting

So, I'm thinking about good R packages to do analyses of #qPCR data. Ones that have caught my eye are tidyqpcr, pcr, qPCRtools. Something that gives some really nice and versatile plots or something that could pipe right into #ggplot is really desired. Anyone have any recommendations? #Rstats

Environmental DNA lets us detect species, but DNA moves & persists in the environment, so a DNA detection may not represent a local, living individual. Our #NewPaper (led by Léonie Suter) uses different DNA fragment lengths to distinguish recently-shed from older krill eDNA

Read the paper here: https://onlinelibrary.wiley.com/doi/full/10.1002/edn3.394

AusAntarctic news summary here: https://www.antarctica.gov.au/news/2023/dna-detector-exposes-how-antarctic-krill-use-habitat/

#eDNA #EnvironmentalDNA #qPCR #Antarctic #krill

Some colleagues and I have been working for a long time on a review of R packages for the analysis of #qPCR experiments, #dPCR experiments and melting curves. What we can find is that there are a lot of packages and we will probably overlook some. I did my last search with https://rdrr.io/ and discovered a lot more.

#rstats #PCR

In 2011, Schwanhäusser, et al. (Nature, 473(7347) measured #absolute #protein & #mRNA levels for 5000 genes in #mamalian cells and informed us 👇

"Protein & mRNA levels correlates 0.41"

Yet, #Reviewer2 demands:

1. #Proteomics validated by #qPCR
2. #RNAseq based #biomarkers

Recently I used https://r-pkg.org/ (
#METACRAN: Search and browse all #CRAN/#R packages). This was a good thing to do, since I discovered R packages related to #qPCR that I haven't heard of before. Since several authors and I are writing a review about #dPCR, qPCR and #meltingcurveanalysis this is good not to forget anybody.

#Rstats
@rstats

Hi Everyone! 🤟

#introduction : I'm an #environmental #engineer , focusing on the #microbiology and #microbialecology of both natural and built systems primarily using #DNA #sequencing , #qPCR , and adjacent techniques. Most of my analytical work is performed in #Rstats.

HDD Centrifuge Puts COVID-19 Testing Lab in a Backpack - Throughout this two-year global COVID-19 nightmare, one thing that has been sorely... - https://hackaday.com/2022/02/05/hdd-centrifuge-puts-covid-19-testing-lab-in-a-backpack/ #medicalhacks #lateralflow #centrifuge #sars-cov-2 #harddrive #covid-19 #e-waster #arduino #testing #lamp #qpcr

Ihr kennt Labore die #PCR machen können oder #qPCR Maschinen haben? Leitet denen das weiter.

https://testtheplanet.com/

#corona #COVID
#coronavirusBLN #coronaupdateBLN

DNA of Lyme disease pathogen found in infected murine bones #Lymedisease #qPCR #bone #osteopenia #microbiology http://ow.ly/ZdBI307otrB

DNA of Lyme disease pathogen found in infected murine bones #Lymedisease #qPCR #bone #osteopenia #microbiology http://ow.ly/kfiy307otnO