#qpcr — Public Fediverse posts
Live and recent posts from across the Fediverse tagged #qpcr, aggregated by home.social.
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Master these essential biotech lab techniques every student must know! From DNA extraction & PCR to Western Blotting—level up your skills with BioResire. Join our WhatsApp channel for free guides: https://whatsapp.com/channel/0029Vb5u2Tp77qVTb1D7bu3r
#BiotechTechniques #DNACultivation #RNAIsolation #AgaroseGel #PCRTechniques #qPCR #GelDocumentation #RestrictionEnzyme #LigationTransformation #PlasmidIsolation #SiteDirectedMutagenesis #ProteinPurification #SDSPAGE #PolyacrylamideGel #Electrophoresis #WesternBlotting
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Master these essential biotech lab techniques every student must know! From DNA extraction & PCR to Western Blotting—level up your skills with BioResire. Join our WhatsApp channel for free guides: https://whatsapp.com/channel/0029Vb5u2Tp77qVTb1D7bu3r
#BiotechTechniques #DNACultivation #RNAIsolation #AgaroseGel #PCRTechniques #qPCR #GelDocumentation #RestrictionEnzyme #LigationTransformation #PlasmidIsolation #SiteDirectedMutagenesis #ProteinPurification #SDSPAGE #PolyacrylamideGel #Electrophoresis #WesternBlotting
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Every biotech student must master these essential lab techniques!
From DNA extraction & PCR to Western blotting at BioResire.
Learn hands-on: [email protected]
#Biotechnology #Biotech #MolecularBiology #PCR #DNAextraction #qPCR #GelElectrophoresis #WesternBlot #RNAPurification #RestrictionDigestion #PlasmidIsolation #SiteDirectedMutagenesis #SDSPAGE #BiotechStudent #LifeSciences #BioResire #BiotechTechniques #LabSkills #Biochemistry #Microbiology -
Every biotech student must master these essential lab techniques!
From DNA extraction & PCR to Western blotting at BioResire.
Learn hands-on: [email protected]
#Biotechnology #Biotech #MolecularBiology #PCR #DNAextraction #qPCR #GelElectrophoresis #WesternBlot #RNAPurification #RestrictionDigestion #PlasmidIsolation #SiteDirectedMutagenesis #SDSPAGE #BiotechStudent #LifeSciences #BioResire #BiotechTechniques #LabSkills #Biochemistry #Microbiology -
Every biotech student must master these essential lab techniques!
From DNA extraction & PCR to Western blotting at BioResire.
Learn hands-on: [email protected]
#Biotechnology #Biotech #MolecularBiology #PCR #DNAextraction #qPCR #GelElectrophoresis #WesternBlot #RNAPurification #RestrictionDigestion #PlasmidIsolation #SiteDirectedMutagenesis #SDSPAGE #BiotechStudent #LifeSciences #BioResire #BiotechTechniques #LabSkills #Biochemistry #Microbiology -
Every biotech student must master these essential lab techniques!
From DNA extraction & PCR to Western blotting at BioResire.
Learn hands-on: [email protected]
#Biotechnology #Biotech #MolecularBiology #PCR #DNAextraction #qPCR #GelElectrophoresis #WesternBlot #RNAPurification #RestrictionDigestion #PlasmidIsolation #SiteDirectedMutagenesis #SDSPAGE #BiotechStudent #LifeSciences #BioResire #BiotechTechniques #LabSkills #Biochemistry #Microbiology -
Every biotech student must master these essential lab techniques!
From DNA extraction & PCR to Western blotting at BioResire.
Learn hands-on: [email protected]
#Biotechnology #Biotech #MolecularBiology #PCR #DNAextraction #qPCR #GelElectrophoresis #WesternBlot #RNAPurification #RestrictionDigestion #PlasmidIsolation #SiteDirectedMutagenesis #SDSPAGE #BiotechStudent #LifeSciences #BioResire #BiotechTechniques #LabSkills #Biochemistry #Microbiology -
Evaluating #primer and #probe #mismatch tolerance in an #Influenza A #matrix gene RT #qPCR using contemporary human and zoonotic strains, https://etidiohnew.blogspot.com/2026/02/evaluating-primer-and-probe-mismatch.html
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Evaluating #primer and #probe #mismatch tolerance in an #Influenza A #matrix gene RT #qPCR using contemporary human and zoonotic strains, https://etidiohnew.blogspot.com/2026/02/evaluating-primer-and-probe-mismatch.html
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Evaluating #primer and #probe #mismatch tolerance in an #Influenza A #matrix gene RT #qPCR using contemporary human and zoonotic strains, https://etidiohnew.blogspot.com/2026/02/evaluating-primer-and-probe-mismatch.html
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Evaluating #primer and #probe #mismatch tolerance in an #Influenza A #matrix gene RT #qPCR using contemporary human and zoonotic strains, https://etidiohnew.blogspot.com/2026/02/evaluating-primer-and-probe-mismatch.html
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Evaluating #primer and #probe #mismatch tolerance in an #Influenza A #matrix gene RT #qPCR using contemporary human and zoonotic strains, https://etidiohnew.blogspot.com/2026/02/evaluating-primer-and-probe-mismatch.html
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Anyone know of a good way to examine EDS files from a Thermofisher RT-PCR machine that doesn't require installing the software suite?
I can probably get the install file. But I fundamentally oppose data locked into a proprietary format for long-term archiving.
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Anyone know of a good way to examine EDS files from a Thermofisher RT-PCR machine that doesn't require installing the software suite?
I can probably get the install file. But I fundamentally oppose data locked into a proprietary format for long-term archiving.
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Anyone know of a good way to examine EDS files from a Thermofisher RT-PCR machine that doesn't require installing the software suite?
I can probably get the install file. But I fundamentally oppose data locked into a proprietary format for long-term archiving.
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Anyone know of a good way to examine EDS files from a Thermofisher RT-PCR machine that doesn't require installing the software suite?
I can probably get the install file. But I fundamentally oppose data locked into a proprietary format for long-term archiving.
-
Anyone know of a good way to examine EDS files from a Thermofisher RT-PCR machine that doesn't require installing the software suite?
I can probably get the install file. But I fundamentally oppose data locked into a proprietary format for long-term archiving.
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Following #rstats packages were maintained and are back on #CRAN now thanks to Andrej Spiess
- #dpcR, 2025-06-18, Digital PCR Analysis <10.32614/CRAN.package.dpcR>
- #MBmca, 2025-06-11, Nucleic Acid Melting Curve Analysis (https://journal.r-project.org/articles/RJ-2013-024/)
- #qpcR, 2025-06-10, Modelling and Analysis of Real-Time PCR Data <doi10.1093/bioinformatics/btn227>
- #PCRedux, 2025-06-13, Quantitative #PCR (#qPCR) Data Mining and Machine Learning Toolkit as Described in <doi:10.21105/Joss.04407> -
Following #rstats packages were maintained and are back on #CRAN now thanks to Andrej Spiess
- #dpcR, 2025-06-18, Digital PCR Analysis <10.32614/CRAN.package.dpcR>
- #MBmca, 2025-06-11, Nucleic Acid Melting Curve Analysis (https://journal.r-project.org/articles/RJ-2013-024/)
- #qpcR, 2025-06-10, Modelling and Analysis of Real-Time PCR Data <doi10.1093/bioinformatics/btn227>
- #PCRedux, 2025-06-13, Quantitative #PCR (#qPCR) Data Mining and Machine Learning Toolkit as Described in <doi:10.21105/Joss.04407> -
Following #rstats packages were maintained and are back on #CRAN now thanks to Andrej Spiess
- #dpcR, 2025-06-18, Digital PCR Analysis <10.32614/CRAN.package.dpcR>
- #MBmca, 2025-06-11, Nucleic Acid Melting Curve Analysis (https://journal.r-project.org/articles/RJ-2013-024/)
- #qpcR, 2025-06-10, Modelling and Analysis of Real-Time PCR Data <doi10.1093/bioinformatics/btn227>
- #PCRedux, 2025-06-13, Quantitative #PCR (#qPCR) Data Mining and Machine Learning Toolkit as Described in <doi:10.21105/Joss.04407> -
Following #rstats packages were maintained and are back on #CRAN now thanks to Andrej Spiess
- #dpcR, 2025-06-18, Digital PCR Analysis <10.32614/CRAN.package.dpcR>
- #MBmca, 2025-06-11, Nucleic Acid Melting Curve Analysis (https://journal.r-project.org/articles/RJ-2013-024/)
- #qpcR, 2025-06-10, Modelling and Analysis of Real-Time PCR Data <doi10.1093/bioinformatics/btn227>
- #PCRedux, 2025-06-13, Quantitative #PCR (#qPCR) Data Mining and Machine Learning Toolkit as Described in <doi:10.21105/Joss.04407> -
Following #rstats packages were maintained and are back on #CRAN now thanks to Andrej Spiess
- #dpcR, 2025-06-18, Digital PCR Analysis <10.32614/CRAN.package.dpcR>
- #MBmca, 2025-06-11, Nucleic Acid Melting Curve Analysis (https://journal.r-project.org/articles/RJ-2013-024/)
- #qpcR, 2025-06-10, Modelling and Analysis of Real-Time PCR Data <doi10.1093/bioinformatics/btn227>
- #PCRedux, 2025-06-13, Quantitative #PCR (#qPCR) Data Mining and Machine Learning Toolkit as Described in <doi:10.21105/Joss.04407> -
Our paper "MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines" is now published in Clinical Chemistry! 📚🔬
This update addresses recent advances in qPCR technology, providing clear recommendations on sample handling, assay design, and data analysis. We emphasize transparency and reproducibility to enhance the reliability of qPCR research.
https://doi.org/10.1093/clinchem/hvaf043 #MIQE2_0 #MIQE #qPCR #ResearchIntegrity #ScientificMethodology 📊🔍
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Our paper "MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines" is now published in Clinical Chemistry! 📚🔬
This update addresses recent advances in qPCR technology, providing clear recommendations on sample handling, assay design, and data analysis. We emphasize transparency and reproducibility to enhance the reliability of qPCR research.
https://doi.org/10.1093/clinchem/hvaf043 #MIQE2_0 #MIQE #qPCR #ResearchIntegrity #ScientificMethodology 📊🔍
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Our paper "MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines" is now published in Clinical Chemistry! 📚🔬
This update addresses recent advances in qPCR technology, providing clear recommendations on sample handling, assay design, and data analysis. We emphasize transparency and reproducibility to enhance the reliability of qPCR research.
https://doi.org/10.1093/clinchem/hvaf043 #MIQE2_0 #MIQE #qPCR #ResearchIntegrity #ScientificMethodology 📊🔍
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Our paper "MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines" is now published in Clinical Chemistry! 📚🔬
This update addresses recent advances in qPCR technology, providing clear recommendations on sample handling, assay design, and data analysis. We emphasize transparency and reproducibility to enhance the reliability of qPCR research.
https://doi.org/10.1093/clinchem/hvaf043 #MIQE2_0 #MIQE #qPCR #ResearchIntegrity #ScientificMethodology 📊🔍
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For the next few months, Dr. Andrej-Nikolai Spiess (https://openalex.org/works?page=1&filter=authorships.author.id%3Aa5027948408&sort=publication_year%3Adesc) will be a guest in my working group.
We are working on a paper where we show that 29 % of papers in top journals like Science, Nature & PNAS were skewed by a single influential data point! Time to rethink our reliance on p-values and explore alternative measures like #dfstat. #reproducibilitycrisis #linearregression #rstats
Moreover, we will work on #qPCR related software like PCRedux (https://joss.theoj.org/papers/10.21105/joss.04407)
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For the next few months, Dr. Andrej-Nikolai Spiess (https://openalex.org/works?page=1&filter=authorships.author.id%3Aa5027948408&sort=publication_year%3Adesc) will be a guest in my working group.
We are working on a paper where we show that 29 % of papers in top journals like Science, Nature & PNAS were skewed by a single influential data point! Time to rethink our reliance on p-values and explore alternative measures like #dfstat. #reproducibilitycrisis #linearregression #rstats
Moreover, we will work on #qPCR related software like PCRedux (https://joss.theoj.org/papers/10.21105/joss.04407)
-
For the next few months, Dr. Andrej-Nikolai Spiess (https://openalex.org/works?page=1&filter=authorships.author.id%3Aa5027948408&sort=publication_year%3Adesc) will be a guest in my working group.
We are working on a paper where we show that 29 % of papers in top journals like Science, Nature & PNAS were skewed by a single influential data point! Time to rethink our reliance on p-values and explore alternative measures like #dfstat. #reproducibilitycrisis #linearregression #rstats
Moreover, we will work on #qPCR related software like PCRedux (https://joss.theoj.org/papers/10.21105/joss.04407)
-
For the next few months, Dr. Andrej-Nikolai Spiess (https://openalex.org/works?page=1&filter=authorships.author.id%3Aa5027948408&sort=publication_year%3Adesc) will be a guest in my working group.
We are working on a paper where we show that 29 % of papers in top journals like Science, Nature & PNAS were skewed by a single influential data point! Time to rethink our reliance on p-values and explore alternative measures like #dfstat. #reproducibilitycrisis #linearregression #rstats
Moreover, we will work on #qPCR related software like PCRedux (https://joss.theoj.org/papers/10.21105/joss.04407)
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Most problems have been fixed in the #PCRedux package. There are still issues with the #rgl package and #Matrix is causing trouble on older platforms (especially #Ubuntu) 🤔. We're still figuring out how to solve the rgl problem, unfortunately it depends on the #qpcR package which calculates some of our key parameters 💡.
More work ahead of us. -
Most problems have been fixed in the #PCRedux package. There are still issues with the #rgl package and #Matrix is causing trouble on older platforms (especially #Ubuntu) 🤔. We're still figuring out how to solve the rgl problem, unfortunately it depends on the #qpcR package which calculates some of our key parameters 💡.
More work ahead of us. -
Most problems have been fixed in the #PCRedux package. There are still issues with the #rgl package and #Matrix is causing trouble on older platforms (especially #Ubuntu) 🤔. We're still figuring out how to solve the rgl problem, unfortunately it depends on the #qpcR package which calculates some of our key parameters 💡.
More work ahead of us. -
Most problems have been fixed in the #PCRedux package. There are still issues with the #rgl package and #Matrix is causing trouble on older platforms (especially #Ubuntu) 🤔. We're still figuring out how to solve the rgl problem, unfortunately it depends on the #qpcR package which calculates some of our key parameters 💡.
More work ahead of us. -
Most problems have been fixed in the #PCRedux package. There are still issues with the #rgl package and #Matrix is causing trouble on older platforms (especially #Ubuntu) 🤔. We're still figuring out how to solve the rgl problem, unfortunately it depends on the #qpcR package which calculates some of our key parameters 💡.
More work ahead of us. -
New publication: Risk assessment of toxic #cyanobacterial blooms in recreational #waters: A comparative study of monitoring #methods. #microsystin #fluorometry #microscopy #qPCR
https://doi.org/10.1016/j.hal.2024.102683 -
New publication: Risk assessment of toxic #cyanobacterial blooms in recreational #waters: A comparative study of monitoring #methods. #microsystin #fluorometry #microscopy #qPCR
https://doi.org/10.1016/j.hal.2024.102683 -
New publication: Risk assessment of toxic #cyanobacterial blooms in recreational #waters: A comparative study of monitoring #methods. #microsystin #fluorometry #microscopy #qPCR
https://doi.org/10.1016/j.hal.2024.102683 -
New publication: Risk assessment of toxic #cyanobacterial blooms in recreational #waters: A comparative study of monitoring #methods. #microsystin #fluorometry #microscopy #qPCR
https://doi.org/10.1016/j.hal.2024.102683 -
#US #FDA, Updates on Highly Pathogenic Avian #Influenza #H5N1 (HPAI), as of April 23 2024, https://www.fda.gov/food/alerts-advisories-safety-information/updates-highly-pathogenic-avian-influenza-hpai
Based on available information, #pasteurization is likely to inactivate the virus, however the process is not expected to remove the presence of viral #particles. Therefore, some of the samples collected have indicated the presence of HPAI using quantitative polymerase chain reaction (#qPCR) testing.
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#US #FDA, Updates on Highly Pathogenic Avian #Influenza #H5N1 (HPAI), as of April 23 2024, https://www.fda.gov/food/alerts-advisories-safety-information/updates-highly-pathogenic-avian-influenza-hpai
Based on available information, #pasteurization is likely to inactivate the virus, however the process is not expected to remove the presence of viral #particles. Therefore, some of the samples collected have indicated the presence of HPAI using quantitative polymerase chain reaction (#qPCR) testing.
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#US #FDA, Updates on Highly Pathogenic Avian #Influenza #H5N1 (HPAI), as of April 23 2024, https://www.fda.gov/food/alerts-advisories-safety-information/updates-highly-pathogenic-avian-influenza-hpai
Based on available information, #pasteurization is likely to inactivate the virus, however the process is not expected to remove the presence of viral #particles. Therefore, some of the samples collected have indicated the presence of HPAI using quantitative polymerase chain reaction (#qPCR) testing.
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#US #FDA, Updates on Highly Pathogenic Avian #Influenza #H5N1 (HPAI), as of April 23 2024, https://www.fda.gov/food/alerts-advisories-safety-information/updates-highly-pathogenic-avian-influenza-hpai
Based on available information, #pasteurization is likely to inactivate the virus, however the process is not expected to remove the presence of viral #particles. Therefore, some of the samples collected have indicated the presence of HPAI using quantitative polymerase chain reaction (#qPCR) testing.
-
#US #FDA, Updates on Highly Pathogenic Avian #Influenza #H5N1 (HPAI), as of April 23 2024, https://www.fda.gov/food/alerts-advisories-safety-information/updates-highly-pathogenic-avian-influenza-hpai
Based on available information, #pasteurization is likely to inactivate the virus, however the process is not expected to remove the presence of viral #particles. Therefore, some of the samples collected have indicated the presence of HPAI using quantitative polymerase chain reaction (#qPCR) testing.
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#OTTO: #OpenSource affordable #liquid handler for #automated #micropipetting:
-applied to generate reproducible #qPCR data
-#Arduino-/#Python-controlled#OpenAccess article: https://doi.org/10.1038/s41598-020-70465-5
Web: https://openliquidhandler.com/ -
#OTTO: #OpenSource affordable #liquid handler for #automated #micropipetting:
-applied to generate reproducible #qPCR data
-#Arduino-/#Python-controlled#OpenAccess article: https://doi.org/10.1038/s41598-020-70465-5
Web: https://openliquidhandler.com/ -
#OTTO: #OpenSource affordable #liquid handler for #automated #micropipetting:
-applied to generate reproducible #qPCR data
-#Arduino-/#Python-controlled#OpenAccess article: https://doi.org/10.1038/s41598-020-70465-5
Web: https://openliquidhandler.com/ -
#OTTO: #OpenSource affordable #liquid handler for #automated #micropipetting:
-applied to generate reproducible #qPCR data
-#Arduino-/#Python-controlled#OpenAccess article: https://doi.org/10.1038/s41598-020-70465-5
Web: https://openliquidhandler.com/ -
#OTTO: #OpenSource affordable #liquid handler for #automated #micropipetting:
-applied to generate reproducible #qPCR data
-#Arduino-/#Python-controlled#OpenAccess article: https://doi.org/10.1038/s41598-020-70465-5
Web: https://openliquidhandler.com/ -
Environmental DNA lets us detect species, but DNA moves & persists in the environment, so a DNA detection may not represent a local, living individual. Our #NewPaper (led by Léonie Suter) uses different DNA fragment lengths to distinguish recently-shed from older krill eDNA
Read the paper here: https://onlinelibrary.wiley.com/doi/full/10.1002/edn3.394
AusAntarctic news summary here: https://www.antarctica.gov.au/news/2023/dna-detector-exposes-how-antarctic-krill-use-habitat/
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Environmental DNA lets us detect species, but DNA moves & persists in the environment, so a DNA detection may not represent a local, living individual. Our #NewPaper (led by Léonie Suter) uses different DNA fragment lengths to distinguish recently-shed from older krill eDNA
Read the paper here: https://onlinelibrary.wiley.com/doi/full/10.1002/edn3.394
AusAntarctic news summary here: https://www.antarctica.gov.au/news/2023/dna-detector-exposes-how-antarctic-krill-use-habitat/
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Environmental DNA lets us detect species, but DNA moves & persists in the environment, so a DNA detection may not represent a local, living individual. Our #NewPaper (led by Léonie Suter) uses different DNA fragment lengths to distinguish recently-shed from older krill eDNA
Read the paper here: https://onlinelibrary.wiley.com/doi/full/10.1002/edn3.394
AusAntarctic news summary here: https://www.antarctica.gov.au/news/2023/dna-detector-exposes-how-antarctic-krill-use-habitat/
-
Environmental DNA lets us detect species, but DNA moves & persists in the environment, so a DNA detection may not represent a local, living individual. Our #NewPaper (led by Léonie Suter) uses different DNA fragment lengths to distinguish recently-shed from older krill eDNA
Read the paper here: https://onlinelibrary.wiley.com/doi/full/10.1002/edn3.394
AusAntarctic news summary here: https://www.antarctica.gov.au/news/2023/dna-detector-exposes-how-antarctic-krill-use-habitat/
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Some colleagues and I have been working for a long time on a review of R packages for the analysis of #qPCR experiments, #dPCR experiments and melting curves. What we can find is that there are a lot of packages and we will probably overlook some. I did my last search with https://rdrr.io/ and discovered a lot more.
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Some colleagues and I have been working for a long time on a review of R packages for the analysis of #qPCR experiments, #dPCR experiments and melting curves. What we can find is that there are a lot of packages and we will probably overlook some. I did my last search with https://rdrr.io/ and discovered a lot more.
