#parp1 — Public Fediverse posts

PARP1 (PDB: 4DQY) — catalytic core resolves NAD⁺ positioning (H133/E134/Mg²⁺) and substrate interface (326–332) as structurally separable sites. E134 as the ADP-ribose acceptor base.

Age-associated DNA damage → PARP1 hyperactivation → NAD⁺ depletion → SIRT1/3 inactivation → mitochondrial dysfunction. A structurally grounded feedforward loop in biological aging.

#PARP1 #NAD #StructuralBiology #LongevityBiology #Aging #DNARepair #Biogerontology #Sirtuins

🧬 Can controlling PARP1 turn CRISPR from a gene scissor into a gene sculptor?

🔗 Influence of PARP1 on CRISPR/Cas9 induced double strand break repair in proliferating cells. Computational and Structural Biotechnology Journal, DOI: https://doi.org/10.1016/j.csbj.2025.10.001

📚 CSBJ: https://www.csbj.org/

#CRISPR #GenomeEditing #Genomics #DNARepair #PARP1 #GeneticEngineering #MolecularBiology #GeneTherapy #CellBiology #SyntheticBiology #TherapeuticEditing