#neuraminidase — Public Fediverse posts

N70S/Y155H Combined #Mutation of #Neuraminidase of Avian #Influenza #H9N2 Virus confers #oseltamivir and #zanamivir #resistance and pathogenicity to Mice, https://etidiohnew.blogspot.com/2026/09/n70sy155h-combined-mutation-of.html

Influenza 🇺🇸

#Orthomyxoviridae #hemagglutinin #infectiousdisease #neuraminidase #theflu

▶️ 1 new picture from NIH BioArt https://commons.wikimedia.org/wiki/File:Influenza_%28NIH_BioArt_858_-_797548%29.svg

Sex differences in #vaccine-induced #neuraminidase cross-recognition impact #H5N1 #dissemination to the lower respiratory tract in mice, https://etidiohnew.blogspot.com/2026/09/sex-differences-in-vaccine-induced.html

Impaired #Neuraminidase and Polymerase Activities Correspond With Limited Aerosol Infectivity of #B3.13 and #D1.1 #H5N1 Lineages in #Human Respiratory Cultures, https://etidiohnew.blogspot.com/2026/08/impaired-neuraminidase-and-polymerase.html

#Cattle and #human #organoids reveal 2.3.4.4b #H5N1 cross-species #transmission potential and #neuraminidase-specific neutralizing #antibodies in humans, https://etidiohnew.blogspot.com/2026/07/cattle-and-human-organoids-reveal-2344b.html

#Antibodies to #influenza A virus #hemagglutinin and #neuraminidase limit egress and alter the physical properties of released virus particles, https://etidiohnew.blogspot.com/2026/06/antibodies-to-influenza-virus.html

Sex differences in #vaccine-induced #neuraminidase cross-recognition and #protection against #H5N1 in mice, https://etidiohnew.blogspot.com/2026/05/sex-differences-in-vaccine-induced.html

Membrane-anchored #influenza #neuraminidase vaccine drives #human-like broadly protective B cell responses, https://etidiohnew.blogspot.com/2026/05/membrane-anchored-influenza.html

Cross-reactive #human #antibody responses to #H5N1 #influenza virus #neuraminidase are shaped by immune history, https://etidiohnew.blogspot.com/2026/05/cross-reactive-human-antibody-responses.html

Serum #neutralisation of #H5N1 clade 2.3.4.4b #influenza is largely mediated by #neuraminidase-directed #antibodies, https://www.thelancet.com/journals/lanmic/article/PIIS2666-5247(26)00086-8/fulltext

2/3 of adults tested had antibodies neutralising H5N1 suggesting that they could protect vs this virus. ... this cross-neutralising activity was mediated by N1 NA-directed abs, suggesting importance of NA in the design of future influenza vaccines.

#Population #immunity to clade 2.3.4.4b #H5N1 is dominated by anti - #neuraminidase #antibodies, https://etidiohnew.blogspot.com/2026/04/population-immunity-to-clade-2344b-h5n1.html

#Oseltamivir aziridines are potent #influenza #neuraminidase #inhibitors and imaging agents, https://etidiohnew.blogspot.com/2026/03/oseltamivir-aziridines-are-potent.html

#Immunity to #hemagglutinin and #neuraminidase results in additive reductions in #airborne #transmission of #influenza #H1N1 virus in #ferrets, https://etidiohnew.blogspot.com/2026/03/immunity-to-hemagglutinin-and.html

A newly emergent N1 #neuraminidase associated with clade 2.3.4.4b highly pathogenic avian #influenza #H5 viruses in North #America, https://etidiohnew.blogspot.com/2026/03/a-newly-emergent-n1-neuraminidase.html

Amino acid #mutations K54E and S154P in the #neuraminidase attenuate #H3N2 #canine #influenza virus in mice, https://etidiohnew.blogspot.com/2026/02/amino-acid-mutations-k54e-and-s154p-in.html

Intra-patient #neuraminidase #mutations in avian #H5N1 #influenza virus reduce #sialidase activity to complement weaker hemagglutinin binding and facilitate #human infection, https://etidiohnew.blogspot.com/2026/01/intra-patient-neuraminidase-mutations.html

#Evolution of #antibody cross-reactivity to #influenza #H5N1 #neuraminidase from an N2-specific germline, https://etidiohnew.blogspot.com/2025/11/evolution-of-antibody-cross-reactivity.html

#Evolution of #antibody cross-reactivity to #influenza #H5N1 #neuraminidase from an N2-specific germline, https://etidiohnew.blogspot.com/2025/10/evolution-of-antibody-cross-reactivity.html

Cross-reactive #neuraminidase inhibition #antibodies against #H5N1 by consecutive #influenza A imprinting cohorts of the past century: population-based serosurvey, British Columbia, #Canada, https://etidiohnew.blogspot.com/2025/09/cross-reactive-neuraminidase-inhibition.html

Cross-reactive #human #antibody responses to #H5N1 #influenza virus #neuraminidase are shaped by immune history, https://etidiohnew.blogspot.com/2025/09/cross-reactive-human-antibody-responses.html

#Neuraminidase #imprinting and the age-related #risk of zoonotic #influenza, https://etidiohnew.blogspot.com/2025/07/neuraminidase-imprinting-and-age.html

A 15-year study of #neuraminidase #mutations and the increasing of S247N mutation in #Spain, https://etidiohnew.blogspot.com/2025/07/a-15-year-study-of-neuraminidase.html

#Evolution of #antibody cross-reactivity to #influenza #H5N1 #neuraminidase from an N2-specific germline, https://etidiohnew.blogspot.com/2025/06/evolution-of-antibody-cross-reactivity.html

#Global #update on susceptibilities of #influenza viruses to #neuraminidase #inhibitors and the cap-dependent endonuclease inhibitor #baloxavir, 2020–2023, https://etidiohnew.blogspot.com/2025/06/global-update-on-susceptibilities-of.html

Impact of #serum versus #anticoagulant-containing #plasma on #influenza virus #neuraminidase-based serological assays, https://etidiohnew.blogspot.com/2025/06/impact-of-serum-versus-anticoagulant.html

#Phylogenetic Analysis and Spread of HPAI #H5N1 in Middle Eastern Countries Based on #Hemagglutinin and #Neuraminidase Gene Sequences, https://etidiohnew.blogspot.com/2025/05/phylogenetic-analysis-and-spread-of.html

Elucidating the Characteristics and #Clonal Evolutionary #Trajectory of #Influenza #Neuraminidase Broadly Reactive B Cell, https://etidiohnew.blogspot.com/2025/03/elucidating-characteristics-and-clonal.html

Clade 2.3.4.4b #H5N1 #neuraminidase has a long #stalk, which is in contrast to most highly pathogenic H5N1 viruses circulating between 2002 and 2020, https://etidiohnew.blogspot.com/2025/02/clade-2344b-h5n1-neuraminidase-has-long.html

#Neuraminidase #reassortment and #oseltamivir #resistance in clade 2.3.4.4b A(#H5N1) viruses circulating among #Canadian #poultry, 2024, https://etidiohnew.blogspot.com/2025/02/neuraminidase-reassortment-and.html

Isolates from 8 farms reveal a mutation in the neuraminidase protein (H275Y) that is exceptionally rare among clade 2.3.4.4b viruses (present in 0.045% of publicly available clade 2.3.4.4b isolates). NA-H275Y is a well-known marker of resistance to the neuraminidase inhibitor oseltamivir.

#Neuraminidase #reassortment and #oseltamivir #resistance in clade 2.3.4.4b A(#H5N1) viruses circulating among #Canadian #poultry, 2024, https://etidiohnew.blogspot.com/2025/02/neuraminidase-reassortment-and.html

Deglycosylation and #truncation in the #neuraminidase #stalk are functionally equivalent in enhancing the #pathogenicity of a HPAI virus in #chickens, https://etidiohnew.blogspot.com/2025/02/deglycosylation-and-truncation-in.html

The #evolution of #hemagglutinin-158 and #neuraminidase-88 #glycosylation sites modulates antigenicity and #pathogenicity of clade 2.3.2.1 #H5N1 avian #influenza viruses, https://etidiohnew.blogspot.com/2024/12/the-evolution-of-hemagglutinin-158-and.html

The #antigenic #landscape of #N1 #neuraminidase in #human #influenza A virus strains isolated between 2009 and 2020, BioRxIV, https://www.biorxiv.org/content/10.1101/2024.12.10.627724v1

The resulting neuraminidase inhibition pattern revealed two antigenic groups that circulated in this period. A machine learning method identified K432E and I321V as key determinants of N1 neuraminidase antigenicity.

Boosting #neuraminidase #immunity in the presence of #hemagglutinin with the next generation of #influenza #vaccines

Source: npj Vaccines, AbstractNeuraminidase (NA), the second most abundant surface glycoprotein on the influenza virus, plays a key role in viral replication and propagation. Despite growing evidence showing that NA-specific antibodies correlate with resistance to disease in humans, current licensed vaccines focus almost entirely on the hemagglutinin…

https://etidioh.wordpress.com/2024/11/20/boosting-neuraminidase-immunity-in-the-presence-of-hemagglutinin-with-the-next-generation-of-influenza-vaccines/

Loop #Grafting Improves #Neuraminidase Protein #Vaccine #Production

Source: BioRxIV, AbstractInfluenza neuraminidase is a crucial target for protective antibodies, but development of recombinant neuraminidase protein as a vaccine has been held back by instability and variable expression. We have taken a pragmatic approach to improving expression and stability of neuraminidase by grafting antigenic surface loops from low-expressing neuraminidase proteins onto…

https://etidioh.wordpress.com/2024/10/17/loop-grafting-improves-neuraminidase-protein-vaccine-production/

A #human #monoclonal #antibody targeting the monomeric #N6 #neuraminidase confers protection against avian #H5N6 #influenza virus infection

Source: Nature Communications, AbstractThe influenza neuraminidase (NA) is a potential target for the development of a next-generation influenza vaccine, but its antigenicity is not well understood. Here, we isolate an anti-N6 human monoclonal antibody, named 18_14D, from an H5N6 avian influenza virus (AIV) infected patient. The…

https://etidioh.wordpress.com/2024/10/15/a-human-monoclonal-antibody-targeting-the-monomeric-n6-neuraminidase-confers-protection-against-avian-h5n6-influenza-virus-infection/

Triton X-100-treated virus-based ELLA demonstrates discordant #antigenic #evolution of #influenza B virus #hemagglutinin and #neuraminidase

Source: Journal of Viroloyg, ABSTRACTNeuraminidase (NA)-specific antibodies have been associated with protection against influenza and thus NA is considered a promising target for next-generation vaccines against influenza A (IAV) and B viruses (IBV). NA inhibition (NI) by antibodies is typically assessed using an enzyme-linked…

https://etidioh.wordpress.com/2024/10/03/triton-x-100-treated-virus-based-ella-demonstrates-discordant-antigenic-evolution-of-influenza-b-virus-hemagglutinin-and-neuraminidase/

#Hemagglutinin and #neuraminidase of a non-pathogenic #H7N7 avian #influenza virus coevolved during acquisition of intranasal #pathogenicity in #chickens, Arch Virol.: https://link.springer.com/article/10.1007/s00705-024-06118-z

This study demonstrates that viruses that are highly pathogenic when administered intranasally require additional adaptations for increased pathogenicity to be highly lethal to intranasally infected chickens.

#Targets of #influenza #Human T cell #response are mostly conserved in #H5N1, BioRxIV: https://www.biorxiv.org/content/10.1101/2024.09.09.612060v1

Based on these observations, and overall similarity of #neuraminidase (NA) N1 subtype encoded in both #HPAI and seasonal #H1N1 influenza virus as well as cross-reactive group 1 HA #stalk-reactive #antibodies, we expect that a degree of pre-existing #immunity is present in general human population that could blunt severity of human H5N1 infections.

Fc-dependent protective #efficacy of non-inhibitory #antibodies targeting #influenza A virus #neuraminidase is limited by epitope availability, BioRxIV: https://www.biorxiv.org/content/10.1101/2024.09.03.611041v1

We aimed to isolate Abs with cross-subtype reactivity vs human #H1N1 & #H3N2 IAVs from transgenic #mice bearing genes encoding human Ig variable regions. We immunized these mice with recombinant N1 & N2 NA proteins, presenting them either as unconjugated soluble proteins or conjugated protein nanoparticles.

Whole-Genome #Analysis of #Influenza #H1N1pdm09 Viruses Isolated from ILI #Outpatients in #Myanmar & CA #Oseltamivir-Resistant Strains Present from 2015 to 2019 https://pubmed.ncbi.nlm.nih.gov/39205274/?utm_source=Feedly&utm_medium=rss&utm_campaign=None&utm_content=10ykRO9og5pGdo51l9DEPEpOJ3DVFIn__QK2QPM3dci1C1dEYq&fc=None&ff=20240831103754&v=2.18.0.post9+e462414

3 viruses possessed the #H275Y substitution in #neuraminidase protein, appearing to be community-acquired without prior administration of neuraminidase inhibitors. These viruses exhibited highly reduced susceptibility to #oseltamivir and #peramivir.

A Computationally Optimized Broadly Reactive #Hemagglutinin and #Neuraminidase #Vaccine Boosts #Antibody-Secreting Cells and Induces a Robust #Serological Response, Preventing #Lung #Damage in a Pre-Immune #Model https://pubmed.ncbi.nlm.nih.gov/39066344/?utm_source=Feedly&utm_medium=rss&utm_campaign=None&utm_content=1LGAx__djGAJ9poBzWGqPtjEbBDtz4ipSRRPUplmgHEyyF_Ihj&fc=None&ff=20240727133152&v=2.18.0.post9+e462414

... the combination of #H2, #H5, #H7, #N1, and #N2 #COBRA #antigens presents a potential for the formulation of a universal influenza virus vaccine.

#Genetic & #molecular characterization of #H9N2 avian #influenza viruses in #Yunnan Province, SW #China, Poul Sci.: https://www.sciencedirect.com/science/article/pii/S0032579124006199?via%3Dihub

Analysis of these #aminoacid sites suggests that H9N2 influenza viruses in Yunnan continue to mutate and adapt to #mammals & are sensitive to #neuraminidase #inhibitors but resistant to #adamantanes. It is necessary to strengthen surveillance of AIV H9N2 subtypes in poultry and LPMs in Yunnan to further understand their genetic diversity.

#US #CDC, #HPAI #H5N1 in #Humans: Update, https://www.cdc.gov/bird-flu/spotlights/h5n1-response-07122024.html

...despite extensive efforts, CDC has been unable to #sequence or isolate virus from recent human case in #Colorado {linked to #cows}. Attempts to #sequence viral #RNA from Colorado case were unsuccessful most likely due to insufficient virus in #clinical sample. As a result, CDC has not been able to conclusively determine the #neuraminidase (NA) subtype of the virus.

Highly pathogenic avian influenza A (H5N1) virus outbreak in Peru in 2022–2023, Infect Med (Beijing), https://www.sciencedirect.com/science/article/pii/S2772431X24000224?via%3Dihub

2022–23 #outbreak of #HPAI in Peru is associated with 2.3.4.4b clade. Peruvian #samples share same common #ancestor with #Chile & are grouped in same #cluster, including #birds and #mammals. Emergency #mutations were found in #hemagglutinin and #neuraminidase genes, some depending on type of host.

Broadly protective bispecific #antibodies that simultaneously target #influenza virus #hemagglutinin and #neuraminidase, mBio: https://journals.asm.org/doi/10.1128/mbio.01085-24

#Infection by the influenza virus remains a global #health burden. The approaches utilized here to augment the activity of broadly protective influenza virus antibodies may lead to a new class of immunotherapies with enhanced activity.

#Structural and #functional characterization of avian #influenza #H9N2 virus #neuraminidase with a #combination of five novel #mutations, Arch Biochem Biophys.: https://www.sciencedirect.com/science/article/abs/pii/S0003986124001607?via%3Dihub

We report combination of novel mutations near the NA active site of an H9N2 strain. The mutations altered surface properties leading to increased substrate binding.

#CD388: A universally protective #Drug-Fc Conjugate that targets #influenza virus #neuraminidase, BioRxIV, Pre-Print, https://www.biorxiv.org/content/10.1101/2024.06.04.597465v1?rss=1

Here, we describe CD388, a first-in-class #antiviral drug-Fc conjugate (DFC) in clinical #trials for seasonal influenza #prevention (NCT05285137 and NCT05523089).

#Avian #Influenza A(#H5N1) #US #Situation #Update and CDC Activities: One Virus Isolated from #Cow showed reduce #sensitivity to #neuraminidase #inhibitors, https://www.cdc.gov/flu/avianflu/spotlights/2023-2024/avian-situation-update.htm

CDC found one virus from a cow with a marker known to be associated with reduced susceptibility to the neuraminidase inhibitors (a change at NA-T438I). This change has been seen rarely in the past in H5N1 viruses isolated from wild birds and poultry.

#Impact of the #H274Y #Substitution on N1, N4, N5, and N8 #Neuraminidase Enzymatic Properties and Expression in Reverse Genetic #Influenza A Viruses https://pubmed.ncbi.nlm.nih.gov/38543754/?utm_source=Feedly&utm_medium=rss&utm_campaign=None&utm_content=10ykRO9og5pGdo51l9DEPEpOJ3DVFIn__QK2QPM3dci1C1dEYq&fc=None&ff=20240328100816&v=2.18.0.post9+e462414

The impact of the H274Y-NA substitution on viral NA expression was different according to the studied NA.

#Resistance profiles for the investigational #neuraminidase #inhibitor AV5080 in #influenza a and B viruses https://pubmed.ncbi.nlm.nih.gov/37567255/?utm_source=Feedly&utm_medium=rss&utm_campaign=None&utm_content=1LGAx__djGAJ9poBzWGqPtjEbBDtz4ipSRRPUplmgHEyyF_Ihj&fc=None&ff=20230812112307&v=2.17.9.post6+86293ac #science #research #antivirals #H1N1 #H3N2 #H7N9 #H5N1