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#ivt — Public Fediverse posts

Live and recent posts from across the Fediverse tagged #ivt, aggregated by home.social.

  1. In today’s #positivenews it’s been confirmed that the heating system I was repairing for the old guy who lives up the road is fully functioning. It’s a challenge to repair electronics without access to schematics and other data, thankfully this time I managed to get him sorted and get him warm again. #asManyWinsAsPossible #repair #repaircafe #aberdeenshire #Rego600 #IVT #heatpump

  2. Ugh, someone botched the #WikiPedia article on #IVT 's. It's prefixed with:

    > This article is about the general concept. For its implementation found in x86 processors, see Interrupt descriptor table.

    And then the second paragraph is completely architecture specific with a huge ass table of supposed vector numbers.

    en.wikipedia.org/wiki/Interrup

  3. Help! I have a problem with #IVT in vitro transcription:
    I created a set of plasmids with T7 promoter & poly-A tail. my gene (~4kb) is inserted in between. I have several variations of the same gene. In all cases, I get 2 products: the correct one and a shorter (~2.8kb) one.
    Any idea why? How do I solve it?

    I use the HiScribe T7 High Yield RNA Synthesis Kit (NEB) with 3"-O-Me-m7G(5")ppp(5")G RNA Cap Structure Analog.
    IVT for GFP works well with just one product of correct size.

  4. #MathMonday I love it when there are useful, “everyday”, applications of a mathematical theorem. Here’s one of my favorites: Take a level table wobbling on uneven ground. The Intermediate Value Theorem guarantees that rotating the table in a circle, less than a quarter turn (for a 4-legged table), will find a spot where the table sits perfectly without wobbling! No need to shove napkins under the “short” leg. (#Proof available upon request.) #calculus #IVT