#flowcytometry — Public Fediverse posts

In one of the other @[email protected] papers from the same researchers, there are unexpected similarities between some of the #FlowCytometry panels. As least, I think they are unexpected. Some regions look identical, others are different. pubpeer.com/publications...

Excited to officially announce our free "Cytometry in R" mini-course, aimed at coding beginners, will be starting up the first week of February! 🥳 It will be offered both online and in-person at the University of Maryland, Baltimore. 1500 people have signed up!!! #flowcytometry #rstats #quarto 1/7

Innovationen durch Unzufriedenheit mit dem Status quo: Das Team um Oliver T. Burton, James Dooley und Adrian Liston der Cambridge Universität nutzt Spülmittel zur Herstellung eines Puffers, um die Balance zwischen Zellfixierung und Durchlässigkeit in der Durchflusszytometrie zu verbessern. Mehr dazu im Artikel von Andrea Pitzschke:
👉 https://www.laborjournal.de/editorials/3373.php

#flowcytometry #liefsciences #laborjournal

@BioInfoCambs
@Psy_Fer_

Innovationen durch Unzufriedenheit mit dem Status quo: Das Team um Oliver T. Burton, James Dooley und Adrian Liston der Cambridge Universität nutzt Spülmittel zur Herstellung eines Puffers, um die Balance zwischen Zellfixierung und Durchlässigkeit in der Durchflusszytometrie zu verbessern. Mehr dazu im Artikel von Andrea Pitzschke:
👉 https://www.laborjournal.de/editorials/3373.php

#flowcytometry #liefsciences #laborjournal

@BioInfoCambs
@Psy_Fer_

#ImageForensics #FlowCytometry Can you spot some problems here? Find at least two.

Research logistician/Engineer position (with PhD) in flow cytometry

UNamur (Université de Namur)

#JOB #ALERT: #UNamur seeks to hire an #engineer for its #flowcytometry facility! If you have a #PhD are expert in #FACS, this job is for you :-)

See the full job description on jobRxiv: https://jobrxiv.org/job/unamur-universite-de-namur-27778-research-logist...
https://jobrxiv.org/job/unamur-universite-de-namur-27778-research-logistician-engineer-position-with-phd-in-flow-cytometry/?feed_id=73170

Research logistician/Engineer position (with PhD) in flow cytometry

UNamur (Université de Namur)

#JOB #ALERT: #UNamur seeks to hire an #engineer for its #flowcytometry facility! If you have a #PhD are expert in #FACS, this job is for you :-)

See the full job description on jobRxiv: https://jobrxiv.org/job/unamur-universite-de-namur-27778-research-logist...
https://jobrxiv.org/job/unamur-universite-de-namur-27778-research-logistician-engineer-position-with-phd-in-flow-cytometry/?feed_id=73170

Research logistician/Engineer position (with PhD) in flow cytometry

UNamur (Université de Namur)

#JOB #ALERT: #UNamur seeks to hire an #engineer for its #flowcytometry facility! If you have a #PhD are expert in #FACS, this job is for you :-)

See the full job description on jobRxiv: https://jobrxiv.org/job/unamur-universite-de-namur-27778-research-logist...
https://jobrxiv.org/job/unamur-universite-de-namur-27778-research-logistician-engineer-position-with-phd-in-flow-cytometry/?feed_id=72533

Research logistician/Engineer position (with PhD) in flow cytometry

UNamur (Université de Namur)

#JOB #ALERT: #UNamur seeks to hire an #engineer for its #flowcytometry facility! If you have a #PhD are expert in #FACS, this job is for you :-)

See the full job description on jobRxiv: https://jobrxiv.org/job/unamur-universite-de-namur-27778-research-logist...
https://jobrxiv.org/job/unamur-universite-de-namur-27778-research-logistician-engineer-position-with-phd-in-flow-cytometry/?feed_id=72533

Macrophages are essential immune cells that reside in all tissues. Some macrophages arise from monocytes in the blood, but during fetal development they also come from a unique pathway!

We still have a lot to learn, but here I'll describe how Zhao et al. (2023) used mouse models to uncover hints about which progenitor cells create tissue-macrophages.

https://doi.org/10.1016/j.celrep.2023.113364

#Macrophages #MouseModel #Immunology #Immunity #scRNAseq #FlowCytometry #Research #JournalArticle #JournalClub

1/9🧵

Macrophages are essential immune cells that reside in all tissues. Some macrophages arise from monocytes in the blood, but during fetal development they also come from a unique pathway!

We still have a lot to learn, but here I'll describe how Zhao et al. (2023) used mouse models to uncover hints about which progenitor cells create tissue-macrophages.

https://doi.org/10.1016/j.celrep.2023.113364

#Macrophages #MouseModel #Immunology #Immunity #scRNAseq #FlowCytometry #Research #JournalArticle #JournalClub

1/9🧵

@biorxiv_bioinfo

Very cool #Preprint from the lab of Jo Jones at #UniversityOfCambridge I've never used #JuliaLang, but this appears to make a lot of the analyses that our lab has hard-coded into a very nifty interactive web-app!!

Really awesome way to make complex #FlowCytometry analysis accessible when built-in tools to #FlowJo don't quite deliver!

#Immunology

Incredible work in this preprint by Konecny, Mage, Tyznik, Prlic, and Mair developing a 50(!) color flow cytometry panel for human immunophenotyping:

https://www.biorxiv.org/content/10.1101/2023.12.14.571745v1

#FlowCytometry #immunology

New from me:

Best Practices in #Plant #FlowCytometry

https://onlinelibrary.wiley.com/doi/abs/10.1002/cyto.a.24798

New from me:

Best Practices in #Plant #FlowCytometry

https://onlinelibrary.wiley.com/doi/abs/10.1002/cyto.a.24798

Really cool application of Infinity Flow to #MouseModel #Tcells, which allows readout/prediction of >200 surface epitopes on single cells via #FlowCytometry.

https://doi.org/10.1016/j.immuni.2023.06.005

By @maxevrard
#Immunology #TissueImmunity

Computational Scientist

Icahn School of Medicine at Mount Sinai

Come join our team as a #computationalScientist: #multiOmics modeling of #myeloma, including #singleCell, #spatialTranscriptomics and #flowCytometry

See the full job description on jobRxiv: https://jobrxiv.org/job/icahn-school-of-medicine-at-mount-sinai-27778-computational-scientist-5/?feed_id=47998

#ScienceJobs #hi...
https://jobrxiv.org/job/icahn-school-of-medicine-at-mount-sinai-27778-computational-scientist-5/?feed_id=47998

Computational Scientist

Icahn School of Medicine at Mount Sinai

Come join our team as a #computationalScientist: #multiOmics modeling of #myeloma, including #singleCell, #spatialTranscriptomics and #flowCytometry

See the full job description on jobRxiv: https://jobrxiv.org/job/icahn-school-of-medicine-at-mount-sinai-27778-computational-scientist-5/?feed_id=47998

#ScienceJobs #hi...
https://jobrxiv.org/job/icahn-school-of-medicine-at-mount-sinai-27778-computational-scientist-5/?feed_id=47998

Postdoctoral Researcher

Icahn School of Medicine at Mount Sinai

Come join our team as a #bioinformatics postdoc: #multiOmics modeling of #myeloma, including #singleCell, #spatialTranscriptomics and #flowCytometry

See the full job description on jobRxiv: https://jobrxiv.org/job/icahn-school-of-medicine-at-mount-sinai-27778-postdoctoral-researcher-4/?feed_id=47893

#ScienceJobs #hi...
https://jobrxiv.org/job/icahn-school-of-medicine-at-mount-sinai-27778-postdoctoral-researcher-4/?feed_id=47893

Postdoctoral Researcher

Icahn School of Medicine at Mount Sinai

Come join our team as a #bioinformatics postdoc: #multiOmics modeling of #myeloma, including #singleCell, #spatialTranscriptomics and #flowCytometry

See the full job description on jobRxiv: https://jobrxiv.org/job/icahn-school-of-medicine-at-mount-sinai-27778-postdoctoral-researcher-4/?feed_id=47893

#ScienceJobs #hi...
https://jobrxiv.org/job/icahn-school-of-medicine-at-mount-sinai-27778-postdoctoral-researcher-4/?feed_id=47893

Computational Scientist

Icahn School of Medicine at Mount Sinai

Come join our team as a #computationalScientist: #multiOmics modeling of #myeloma, including #singleCell, #spatialTranscriptomics and #flowCytometry

See the full job description on jobRxiv: https://jobrxiv.org/job/icahn-school-of-medicine-at-mount-sinai-27778-computational-scientist-5/?feed_id=45913

#ScienceJobs #hi...
https://jobrxiv.org/job/icahn-school-of-medicine-at-mount-sinai-27778-computational-scientist-5/?feed_id=45913

Computational Scientist

Icahn School of Medicine at Mount Sinai

Come join our team as a #computationalScientist: #multiOmics modeling of #myeloma, including #singleCell, #spatialTranscriptomics and #flowCytometry

See the full job description on jobRxiv: https://jobrxiv.org/job/icahn-school-of-medicine-at-mount-sinai-27778-computational-scientist-5/?feed_id=45913

#ScienceJobs #hi...
https://jobrxiv.org/job/icahn-school-of-medicine-at-mount-sinai-27778-computational-scientist-5/?feed_id=45913

Tools (#preprint)

"Our platform is built around a commercial instrument and integrates the handling and transfer of samples to and from the instrument, autonomous control of the instrument's software, and the algorithmic generation of sorting gates, resulting in walkaway functionality."

#FACS #FlowCytometry

https://www.biorxiv.org/content/10.1101/2023.03.24.534165v1

Tools (#preprint)

"Our platform is built around a commercial instrument and integrates the handling and transfer of samples to and from the instrument, autonomous control of the instrument's software, and the algorithmic generation of sorting gates, resulting in walkaway functionality."

#FACS #FlowCytometry

https://www.biorxiv.org/content/10.1101/2023.03.24.534165v1

Anybody know about anything cool happening in #FlowCytometry lately? I want to hear some neat stuff about #SingleCellAnalysis

Time for an #Introduction

This is the mastodon account for DartLab, the Immune Monitoring and Flow Cytometry Shared Resource at the Dartmouth Cancer Center in Lebanon, New Hampshire.

We do work in #Immunology, #FlowCytometry, #Immunotherapy, #Cancer, #Autoimmunity, and #InfectiousDiseases (including #COVID). Looking forward to connecting with other #SharedResources and #CoreFacilities.

This account is run by @mielcarz .

Time for an #Introduction

This is the mastodon account for DartLab, the Immune Monitoring and Flow Cytometry Shared Resource at the Dartmouth Cancer Center in Lebanon, New Hampshire.

We do work in #Immunology, #FlowCytometry, #Immunotherapy, #Cancer, #Autoimmunity, and #InfectiousDiseases (including #COVID). Looking forward to connecting with other #SharedResources and #CoreFacilities.

This account is run by @mielcarz .

⚡️ We are looking for a Research assistant (m/f/d) for our #FlowCytometry & #Cell Sorting group (headed by @MissesThiel). If you have XP in multiparameter flow cytometry & cell sorting by #FACS, check below & apply until February 18, 2023!

#berlinjob

https://www.molgen.mpg.de/4593631/job_full_offer_19755798

Assessment of #Tcell proliferation and other characteristics over a period of ten year. Wow! Fun read.

From the abstract by Soerens et al.: "These observations provide a model to better understand memory cell differentiation, exhaustion, cancer and ageing, and show that functionally competent T cells can retain the potential for extraordinary population expansion and longevity well beyond their organismal lifespan."

Found via @biomiha and @billjeffries

#RNAseq #FlowCytometry #Tcells

Are you interested in Immunology and do you want to come to Germany?
1 year Marie Curie Fellowship for a Predoctoral Researcher @[email protected]
#macrophages #Tregs #FlowCytometry Please RT
See details
👇 👇 👇
https://euraxess.ec.europa.eu/jobs/59357

Many thanks to Dr. Alice Yue and all attendees for making the first edition of the Flow Cytometry analysis in R/Bioconductor a great success!

Best of luck with the analysis of your data!

#Rstats #Bioconductor #flowcytometry

Many thanks to Dr. Alice Yue and all attendees for making the first edition of the Flow Cytometry analysis in R/Bioconductor a great success!

Best of luck with the analysis of your data!

#Rstats #Bioconductor #flowcytometry

If you can already use R, you can skip the tutorial and get the repository straight from GitHub. We have separate scripts optimised for #flowcytometry / #masscytometry or #scSeq. As well as the stats there are nice data visualisation tools included. 4/6 https://github.com/AdrianListon/Cross-Entropy-test

We have an exciting #computational paper out in @[email protected]. Ever use a #tSNE or #UMAP in #scSeq, #flowcytometry or #masscytometry? It doesn't have to be just a pretty picture anymore - we've developed a statistical test to check for differences. 1/6

https://www.cell.com/cell-reports-methods/fulltext/S2667-2375(22)00295-8

We have an exciting #computational paper out in @[email protected]. Ever use a #tSNE or #UMAP in #scSeq, #flowcytometry or #masscytometry? It doesn't have to be just a pretty picture anymore - we've developed a statistical test to check for differences. 1/6

https://www.cell.com/cell-reports-methods/fulltext/S2667-2375(22)00295-8

Our first edition of the Flow Cytometry course with Dr. Alice Yue is now underway!
We will learn about best practices for how to analyze flow cytometry data using R/Bioconductor.

#FlowCytometry #Rstats @Bioconductor #DataScience #CancerResearch

Our first edition of the Flow Cytometry course with Dr. Alice Yue is now underway!
We will learn about best practices for how to analyze flow cytometry data using R/Bioconductor.

#FlowCytometry #Rstats @Bioconductor #DataScience #CancerResearch

Next week we will run the first edition of the #FlowCytometry analysis with #Rstats/@Bioconductor

The course is #SoldOut but we will run another edition very soon!

https://physalia-courses.org/courses-workshops/flow-cytometry/

Next week we will run the first edition of the #FlowCytometry analysis with #Rstats/@Bioconductor

The course is #SoldOut but we will run another edition very soon!

https://physalia-courses.org/courses-workshops/flow-cytometry/

⏳There are still a few seats available for some of our courses in January.

🔗https://physalia-courses.org/courses-workshops/

#RShiny #Rstats #Unix #Bioinformatics #FlowCytometry #Programming #SexChromosome #CancerGenomics #GeneSetEnrichment

⏳There are still a few seats available for some of our courses in January.

🔗https://physalia-courses.org/courses-workshops/

#RShiny #Rstats #Unix #Bioinformatics #FlowCytometry #Programming #SexChromosome #CancerGenomics #GeneSetEnrichment

'This article (...) provides detailed protocols for the preparation of single-cell suspensions from various mouse nonlymphoid tissues, including skin, intestine, lung, kidney, mammary glands, oral mucosa and transplantable tumors, and instructions for multiparameter flow cytometry analysis of DC subsets.'

#Immunology #DendriticCells #FACS #FlowCytometry

https://onlinelibrary.wiley.com/journal/15214141

'This article (...) provides detailed protocols for the preparation of single-cell suspensions from various mouse nonlymphoid tissues, including skin, intestine, lung, kidney, mammary glands, oral mucosa and transplantable tumors, and instructions for multiparameter flow cytometry analysis of DC subsets.'

#Immunology #DendriticCells #FACS #FlowCytometry

https://onlinelibrary.wiley.com/journal/15214141

nterested in #FlowCytometry data analysis with #Rstats/ @Bioconductor?

Have a look at our course, in which participants will be introduced to what flow cytometry is and why we use it to analyze cell population composition in biological samples

🔗https://www.physalia-courses.org/courses-workshops/flow-cytometry/

Hey all, as a way of #introduction I'm Dan Mielcarz, I've been online chatting about music since 1994 starting on Usenet in rec.music.phish. I was "mielcarz" on Twitter, boringly enough.

I've had a mastodon account since 2018 but never really used it, and am psyched to have this server appear. I found this guide to migrating my account very useful if anyone is in the same boat: https://codingitwrong.com/2022/10/10/migrating-a-mastodon-account.html

ETA Hashtags: #Phish #Immunology #FlowCytometry #beer #Jazz