#directedevolution — Public Fediverse posts

If you've ever done a bit of #ProteinEngineering or #DirectedEvolution you know that funny things can happen 🤯 – and sometimes it's worth following up!

I'm excited to share that our paper is finally out in Nature Chemistry! 🎉

Read it here:
https://www.nature.com/articles/s41557-024-01490-4

An Enantioselective Biocatalytic C–H Fluorination
Directed evolution used to create a fluorinating enzyme

https://www.chemistryviews.org/an-enantioselective-biocatalytic-c-h-fluorination/

#chemistry #chemistryviews #chemiverse #research #directedevolution #biocatalysis #fluorination

Directed Evolution Used to Create Enzymes That Break Down Plastics:
Polyethylene terephthalate (PET)-depolymerizing enzyme developed using new high-throughput screening

https://www.chemistryviews.org/directed-evolution-used-to-create-enzymes-that-break-down-plastics/

#chemistry #chemistryviews #chemviews #chemiverse #research #directedevolution #breakdownplastic #PlasticPollution

BREAKING: Project Veritas Senior Reporter @Chris_Hartsock Confronts @YouTube Vice President of Global Trust & Safety Over Tech Giant’s Actions to Remove Our @Pfizer #DirectedEvolution Investigation From The Platform

YouTube Insider leaks "Urgent Guidance" document sent to employees on how to handle the Project Veritas Pfizer #DirectedEvolution video "Effective immediately as of 27 January 2023"

"Violates the COVID-19 misinformation policy"

Still waiting for Reuters to weigh in and give a very neutral and fair fact check of the Project Veritas Pfizer video.

Their lack of any conflicting interests here make them the perfect arbiter of truth for people to place their faith in.

#PfizerExposed #DirectedEvolution

"@CNN, rather than covering the biggest news story in America, ran a segment shilling for big pharma."

@TuckerCarlson Covers #DirectedEvolution AGAIN; Gives Update on @YouTube Removing Viral Video

BREAKING: @JamesOKeefeiii gives update on YouTube Removing Critical Mass #DirectedEvolution Video

#DirectedEvolution is a powerful method but has not been easy to do with gigantic #nonribosomal biocatalysts that make important #antibiotics. We have developed a #microfluidics screen that uses liposomes as sensors for membrane active peptides. Bacteria are grown in tiny droplets. If they make gramicidin S, the sensor liposomes burst, liberate calcein, and generate fluorescence. This was tested for screening mutants and may become a useful tool for directed evolution. https://www.biorxiv.org/content/10.1101/2023.01.13.523969v1

BREAKING: @Pfizer Exploring "Mutating" COVID-19 Virus For New Vaccines

"Don't tell anyone this...There is a risk...have to be very controlled to make sure this virus you mutate doesn't create something...the way that the virus started in Wuhan, to be honest."

#DirectedEvolution

@davidschnettler and the Hollfelder-team have used #DirectedEvolution to breed new phosphotriesterases that could be useful for bioremediation or treatment of organophosphate intoxication. Active #enzyme variants were retrieved from large libraries packaged in tiny droplets on a #microfluidics platform.

Now in #jacs
https://pubs.acs.org/doi/full/10.1021/jacs.2c10673

Here, we show that an alternative motif for phosphotriester hydrolysis exists, starting #DirectedEvolution from a metal-independent alpha/beta hydrolase which Pierre-Yves identified using functional #metagenomics:

https://www.nature.com/articles/ncomms10008

Phosphotriesters are so toxic because they irreversibly inhibit the catalytic triad of acetylcholinesterase. So, our starting point is a homologue of exactly this target, just with a cysteine instead of a serine in the catalytic triad. (3/6)

Excited to share my very first paper which was just published in #JACS:

https://pubs.acs.org/doi/10.1021/jacs.2c10673

In this work carried out during my PhD together with Jamie Klein, Tomasz Kaminski, and Pierre-Yves Colin in the Hollfelder Lab at #UniversityofCambridge we evolve a #phosphotriesterase with a new, metal-independent mechanism by using droplet #microfluidics.

Comments and feedback welcome! A thread: (1/6)

#EnzymeEngineering #ProteinEngineering #EnzymeMechanism #DirectedEvolution #Bioremediation

#introduction

Hi, I'm a biochemist enthusiastic about #enzymes, #biocatalysis and #ProteinEngineering & #ProteinEvolution.

I did my PhD with Florian Hollfelder at the #UniversityofCambridge working on #DirectedEvolution of enzymes using droplet #microfluidics. Currently, I am doing a postdoc at #ETHZurich, studying which properties determine a protein’s innovability towards new function.

Looking forward to learning about cool preprints/papers and finding a lively scientific community here!

#introduction

Hi, I'm a biochemist enthusiastic about #enzymes, #biocatalysis and #ProteinEngineering & #ProteinEvolution.

I did my PhD with Florian Hollfelder at the #UniversityofCambridge working on #DirectedEvolution of enzymes using droplet #microfluidics. Currently, I am doing a postdoc at #ETHZurich, studying which properties determine a protein’s innovability towards new function.

Looking forward to learning about cool preprints/papers and finding a lively scientific community here!

#introduction

Hi, I'm a biochemist enthusiastic about #enzymes, #biocatalysis and #ProteinEngineering & #ProteinEvolution.

I did my PhD with Florian Hollfelder at the #UniversityofCambridge working on #DirectedEvolution of enzymes using droplet #microfluidics. Currently, I am doing a postdoc at #ETHZurich, studying which properties determine a protein’s innovability towards new function.

Looking forward to learning about cool preprints/papers and finding a lively scientific community here!

#introduction

Hi, I'm a biochemist enthusiastic about #enzymes, #biocatalysis and #ProteinEngineering & #ProteinEvolution.

I did my PhD with Florian Hollfelder at the #UniversityofCambridge working on #DirectedEvolution of enzymes using droplet #microfluidics. Currently, I am doing a postdoc at #ETHZurich, studying which properties determine a protein’s innovability towards new function.

Looking forward to learning about cool preprints/papers and finding a lively scientific community here!

#introduction

Hi, I'm a biochemist enthusiastic about #enzymes, #biocatalysis and #ProteinEngineering & #ProteinEvolution.

I did my PhD with Florian Hollfelder at the #UniversityofCambridge working on #DirectedEvolution of enzymes using droplet #microfluidics. Currently, I am doing a postdoc at #ETHZurich, studying which properties determine a protein’s innovability towards new function.

Looking forward to learning about cool preprints/papers and finding a lively scientific community here!

Hi everyone! I'm @basktastic on Twitter but am trying out my real name here. My #chembio #cellbio #lipidtime lab at Cornell develops tools for imaging and editing the lipidome, drawing on #bioorthogonal chemistry, #clickchemistry, #chemoproteomics, #optogenetics, #directedevolution, and more. https://baskin.wicmb.cornell.edu

Hi everyone! I'm @basktastic on Twitter but am trying out my real name here. My #chembio #cellbio #lipidtime lab at Cornell develops tools for imaging and editing the lipidome, drawing on #bioorthogonal chemistry, #clickchemistry, #chemoproteomics, #optogenetics, #directedevolution, and more. https://baskin.wicmb.cornell.edu

Hi everyone! I'm @basktastic on Twitter but am trying out my real name here. My #chembio #cellbio #lipidtime lab at Cornell develops tools for imaging and editing the lipidome, drawing on #bioorthogonal chemistry, #clickchemistry, #chemoproteomics, #optogenetics, #directedevolution, and more. https://baskin.wicmb.cornell.edu

Hi everyone! I'm @basktastic on Twitter but am trying out my real name here. My #chembio #cellbio #lipidtime lab at Cornell develops tools for imaging and editing the lipidome, drawing on #bioorthogonal chemistry, #clickchemistry, #chemoproteomics, #optogenetics, #directedevolution, and more. https://baskin.wicmb.cornell.edu

Hi everyone! I'm @basktastic on Twitter but am trying out my real name here. My #chembio #cellbio #lipidtime lab at Cornell develops tools for imaging and editing the lipidome, drawing on #bioorthogonal chemistry, #clickchemistry, #chemoproteomics, #optogenetics, #directedevolution, and more. https://baskin.wicmb.cornell.edu