#cas9 — Public Fediverse posts

https://www.europesays.com/ie/441059/ Methylation-sensitive Cas9 can selectively target DNA in tumor cells #Biochemistry #cancer #Cas9 #Éire #GeneEditing #IE #Ireland #Methylation #oncology #Science

🧬 CRISPR technology is transforming genetics 🔬, enabling precise gene editing for research, diagnostics, and future therapeutic innovations 🚀.

#CRISPR #GeneEditing #Biotechnology #MolecularBiology #Cas9 #Genomics #LifeScienceStudents #ResearchInnovation #BiotechTrends #BioResire

https://www.europesays.com/ie/285674/ A safer gene-editing tool shows promise for transthyretin amyloidosis treatment #Amyloidosis #bacteria #Biotechnology #Cas9 #CRISPR #DNA #Drugs #Efficacy #Éire #Gene #GeneEditing #Genetic #Genetics #Genome #GenomeEditing #Genomic #Heart #HeartFailure #IE #Ireland #Laboratory #LifeScience #MedicalResearch #medicine #MouseModel #Neuropathy #PalindromicRepeats #Protein #Q #Research #RibonucleicAcid #RNA #Science #Technology #Transgenic

Кроим ДНК на Python — CRISPR gRNA finder, Часть II: Скоринг, off-target и реальный ген

В первой части мы написали базовый поиск gRNA с фильтрацией по GC-составу. Работает, но тупо: все кандидаты в диапазоне 40-60% считаются равнозначными. В реальности это не так. Сегодня добавим систему скоринга — будем ранжировать gRNA по качеству, учитывая позицию нуклеотидов и особенности U6-промотора. Потом подключим NCBI BLAST, чтобы проверять кандидатов на off-target: не порежет ли Cas9 что-нибудь лишнее в геноме. Тестировать будем на гене CCR5 — том самом, который отредактировал китайский учёный Хэ Цзянькуй в скандальном эксперименте 2018 года.

https://habr.com/ru/articles/982876/

#днк #cas9 #crispr #генетика #генная_инженерия #биоинформатика #биоинженерия #анализ_днк

Кроим ДНК на Python: CRISPR gRNA finder, Часть I: Введение и базовый поиск

Несколько лет назад я наткнулся на статью про CRISPR‑Cas9 и домашние биолаборатории — люди буквально у себя дома экспериментировали с редактированием генов. Я бэкенд‑разработчик, биологию последний раз открывал в школе, но желание разобраться никуда не делось. В этой статье разберёмся, как работает CRISPR на минимальном уровне, и напишем CLI‑утилиту на Python для поиска потенциальных guide RNA — «наводчиков» для молекулярных ножниц Cas9.

https://habr.com/ru/articles/982664/

#биоинформатика #биоинженерия #cas9 #crispr #днк #анализ_днк #чайникчайнику

Nanostructures boost CRISPR delivery for genetic medicine

With the power to rewrite the genetic code underlying countless diseases, CRISPR holds immense promise to revolutionize medicine…
#NewsBeep #News #Genetics #Biotechnology #Cas9 #Cell #covid-19 #Crispr #dna #Gene #Geneediting #Genes #Genetic #Genome #GenomeEditing #medicine #Nanomedicine #Nanoparticle #Nanoparticles #Nanostructures #Nanotechnology #research #RNA #Science #Therapeutics #UK #UnitedKingdom
https://www.newsbeep.com/uk/110261/

On this day in 2005: CRISPR noted to provide immunity and degrade DNA as means to prevent infection #CRISPR #Cas9 #ThisDayInBiotech

https://www.europesays.com/uk/180085/ Autophagy boosts precision in gene editing #Autophagy #Cas9 #crispr #DNA #Gene #Genetic #Genetics #Genome #GenomeEditing #Homologous #HomologousRecombination #Medicine #Research #Science #Technology #UK #UnitedKingdom

SciTech Chronicles. . . . . . . . .Feb 15th, 2025

https://bit.ly/stc021525

#Diabetes #antibiotic #resistance #infection ##Murchison #airplane #reflection #RFI CRISPR-Cas9 #Cas9 #RNA #metagenomics #Dragonfly #Titan #Methane #Ingenuity #Londinium #tribunal #70AD #preservation

SciTech Chronicles. . . . . . . . .Feb 15th, 2025

https://bit.ly/stc021525

#Diabetes #antibiotic #resistance #infection ##Murchison #airplane #reflection #RFI CRISPR-Cas9 #Cas9 #RNA #metagenomics #Dragonfly #Titan #Methane #Ingenuity #Londinium #tribunal #70AD #preservation

Researchers at the Broad Institute of MIT and Harvard and Cyrus Biotechnology have now #engineered two #CRISPR nucleases, #Cas9 and #Cas12, to mask them from the #immune system.
#Biotechnology #Biomedical #sflorg
https://www.sflorg.com/2025/01/biot01092501.html

Researchers at the Broad Institute of MIT and Harvard and Cyrus Biotechnology have now #engineered two #CRISPR nucleases, #Cas9 and #Cas12, to mask them from the #immune system.
#Biotechnology #Biomedical #sflorg
https://www.sflorg.com/2025/01/biot01092501.html

Researchers at the Broad Institute of MIT and Harvard and Cyrus Biotechnology have now #engineered two #CRISPR nucleases, #Cas9 and #Cas12, to mask them from the #immune system.
#Biotechnology #Biomedical #sflorg
https://www.sflorg.com/2025/01/biot01092501.html

Researchers at the Broad Institute of MIT and Harvard and Cyrus Biotechnology have now #engineered two #CRISPR nucleases, #Cas9 and #Cas12, to mask them from the #immune system.
#Biotechnology #Biomedical #sflorg
https://www.sflorg.com/2025/01/biot01092501.html

Researchers at the Broad Institute of MIT and Harvard and Cyrus Biotechnology have now #engineered two #CRISPR nucleases, #Cas9 and #Cas12, to mask them from the #immune system.
#Biotechnology #Biomedical #sflorg
https://www.sflorg.com/2025/01/biot01092501.html

Bevor ich für @sciencenotes zu Grüner Gentechnik recherchiert habe, hatte ich keine Meinung zu dem Thema. Dann habe ich viele Studien gelesen. Und nun frage ich mich, wovor so viele Menschen Angst haben. Einen Überblick gibt's im #faq.

#GeneEditing #Cas9 #CRISPR

https://sciencenotes.de/faq-was-kann-gruene-gentechnik-und-was-nicht/

What's next in #plant #gene editing?

Our latest #JIPB Brief Comms feature highlights work by Liu et al. who are developing #guanine base editors for G-to-T editing in #rice.

https://doi.org/10.1111/jipb.13729
@wileyplantsci
#PlantSci #BaseEditing #CRISPR #Cas9 #CropSci #botany

What's next in #plant #gene editing?

Our latest #JIPB Brief Comms feature highlights work by Liu et al. who are developing #guanine base editors for G-to-T editing in #rice.

https://doi.org/10.1111/jipb.13729
@wileyplantsci
#PlantSci #BaseEditing #CRISPR #Cas9 #CropSci #botany

On this day in 2005: CRISPR noted to provide immunity and degrade DNA as means to prevent infection #CRISPR #Cas9 #ThisDayInBiotech

après l'achat de toutes les maisons d'édition de France au bénéfice d'une famille de miliardaires, le mégaéditeur acquert aussi le labo du MIT qui fait de l'édition génétique.

#brokenNews #nouvellesPétées #CRISPR #CAS9

après l'achat de toutes les maisons d'édition de France au bénéfice d'une famille de miliardaires, le mégaéditeur acquert aussi le labo du MIT qui fait de l'édition génétique.

#brokenNews #nouvellesPétées #CRISPR #CAS9

après l'achat de toutes les maisons d'édition de France au bénéfice d'une famille de miliardaires, le mégaéditeur acquert aussi le labo du MIT qui fait de l'édition génétique.

#brokenNews #nouvellesPétées #CRISPR #CAS9

après l'achat de toutes les maisons d'édition de France au bénéfice d'une famille de miliardaires, le mégaéditeur acquert aussi le labo du MIT qui fait de l'édition génétique.

#brokenNews #nouvellesPétées #CRISPR #CAS9

Looking to perform genome-wide screens in bacteria? 🦠 Look no further than base editors—with a few tweaks #HIRI’s Beisel lab introduced to enhance the number of targetable sites. #baseediting #CRISPR #Cas9
https://doi.org/10.1093/nar/gkae174

Looking to perform genome-wide screens in bacteria? 🦠 Look no further than base editors—with a few tweaks #HIRI’s Beisel lab introduced to enhance the number of targetable sites. #baseediting #CRISPR #Cas9
https://doi.org/10.1093/nar/gkae174

Looking to perform genome-wide screens in bacteria? 🦠 Look no further than base editors—with a few tweaks #HIRI’s Beisel lab introduced to enhance the number of targetable sites. #baseediting #CRISPR #Cas9
https://doi.org/10.1093/nar/gkae174

Looking to perform genome-wide screens in bacteria? 🦠 Look no further than base editors—with a few tweaks #HIRI’s Beisel lab introduced to enhance the number of targetable sites. #baseediting #CRISPR #Cas9
https://doi.org/10.1093/nar/gkae174

Looking to perform genome-wide screens in bacteria? 🦠 Look no further than base editors—with a few tweaks #HIRI’s Beisel lab introduced to enhance the number of targetable sites. #baseediting #CRISPR #Cas9
https://doi.org/10.1093/nar/gkae174

Genetic diseases: How scientists are working to make DNA repair (almost) a piece of cake
#Health #Science #DNA #Genetics #Genome #CAS9 #CRISPR #Proteins #GeneticDiseases #DNARepair #PrimeEditing #Mutations #MedicalResearch #ProteinDelivery #GeneticResearch
https://the-14.com/genetic-diseases-how-scientists-are-working-to-make-dna-repair-almost-a-piece-of-cake/

Genetic diseases: How scientists are working to make DNA repair (almost) a piece of cake
#Health #Science #DNA #Genetics #Genome #CAS9 #CRISPR #Proteins #GeneticDiseases #DNARepair #PrimeEditing #Mutations #MedicalResearch #ProteinDelivery #GeneticResearch
https://the-14.com/genetic-diseases-how-scientists-are-working-to-make-dna-repair-almost-a-piece-of-cake/

Genetic diseases: How scientists are working to make DNA repair (almost) a piece of cake
#Health #Science #DNA #Genetics #Genome #CAS9 #CRISPR #Proteins #GeneticDiseases #DNARepair #PrimeEditing #Mutations #MedicalResearch #ProteinDelivery #GeneticResearch
https://the-14.com/genetic-diseases-how-scientists-are-working-to-make-dna-repair-almost-a-piece-of-cake/

Genetic diseases: How scientists are working to make DNA repair (almost) a piece of cake
#Health #Science #DNA #Genetics #Genome #CAS9 #CRISPR #Proteins #GeneticDiseases #DNARepair #PrimeEditing #Mutations #MedicalResearch #ProteinDelivery #GeneticResearch
https://the-14.com/genetic-diseases-how-scientists-are-working-to-make-dna-repair-almost-a-piece-of-cake/

Genetic diseases: How scientists are working to make DNA repair (almost) a piece of cake
#Health #Science #DNA #Genetics #Genome #CAS9 #CRISPR #Proteins #GeneticDiseases #DNARepair #PrimeEditing #Mutations #MedicalResearch #ProteinDelivery #GeneticResearch
https://the-14.com/genetic-diseases-how-scientists-are-working-to-make-dna-repair-almost-a-piece-of-cake/

Neue biotechnologische Methoden erlauben punktgenaue Eingriffe in das Erbgut – wie gut lassen sich Veränderungen nachweisen?

Der aktuelle Wissensstand im #BfR2GO: https://www.bfr.bund.de/epaper/bfr2go_02_2023_deutsch/#page=22

#CRISPR #cas9 #GenomeEditing

#Bacteriophages encode anti-CRISPR proteins that inactivate bacterial #CRISPR-Cas systems, allowing successful invasion, replication & integration. This study shows that #antiCRISPR protein AcrIIA1 induces degradation of #Cas9 in human cells #PLOSBiology https://plos.io/3uUGAos

Scientists at Oak Ridge National Laboratory used their expertise in #quantum #biology, artificial intelligence and #bioengineering to improve how #CRISPR #Cas9 #genome editing tools work on #organisms like #microbes that can be modified to produce #renewable #fuels and #chemicals.
#Biology #AI #ArtificialIntelligence #sflorg
https://www.sflorg.com/2023/11/bio11072301.html

Find out what happens when Merkulov et al. combine #Cas9-targeted #Nanopore sequencing (CANS) with the novel pipeline NanoCasTE to trace both #genetically inherited and #somatic #TEIs in plants!
https://doi.org/10.1111/jipb.13555
@wileyplantsci
#JIPB #PlantSci #Arabidopsis #PlantScience #botany

Find out what happens when Merkulov et al. combine #Cas9-targeted #Nanopore sequencing (CANS) with the novel pipeline NanoCasTE to trace both #genetically inherited and #somatic #TEIs in plants!
https://doi.org/10.1111/jipb.13555
@wileyplantsci
#JIPB #PlantSci #Arabidopsis #PlantScience #botany

Find out what happens when Merkulov et al. combine #Cas9-targeted #Nanopore sequencing (CANS) with the novel pipeline NanoCasTE to trace both #genetically inherited and #somatic #TEIs in plants!
https://doi.org/10.1111/jipb.13555
@wileyplantsci
#JIPB #PlantSci #Arabidopsis #PlantScience #botany

Find out what happens when Merkulov et al. combine #Cas9-targeted #Nanopore sequencing (CANS) with the novel pipeline NanoCasTE to trace both #genetically inherited and #somatic #TEIs in plants!
https://doi.org/10.1111/jipb.13555
@wileyplantsci
#JIPB #PlantSci #Arabidopsis #PlantScience #botany

They call it “#skeletal #editing” and they hope it will change their field – and our world.

Inspired in part by the revolutionary genome-editing technology #Crispr-#Cas9, Levin and Sarpong are among a handful of chemists developing new methods to #insert, #delete and #swap individual atoms within molecules.

The word “editing” evokes chemists altering atoms with pairs of nanoscopic tweezers, but that would be far from efficient. “If you wanted to make a mole,” Levin explains, referring to a unit of measurement used in chemistry, “you would have to take that pair of tweezers and do it 10^23 times.”

https://www.theguardian.com/science/2023/aug/05/skeletal-editing-chemistry-molecule-atom-medicine-drug-development-sustainable?CMP=Share_iOSApp_Other

On this day in 2005: CRISPR noted to provide immunity and degrade DNA as means to prevent infection #CRISPR #Cas9 #ThisDayInBiotech

New nanoparticles can perform gene editing in the lungs
Using these RNA-delivery particles, researchers hope to develop new treatments for cystic fibrosis and other lung diseases. #dna #CRISPR #RNA #geneediting #nanoparticles #isogg #cas9 #geneadons #epigenetics
https://news.mit.edu/2023/new-nanoparticles-can-perform-gene-editing-lungs-0330

Nicht selten wirken pathogene #Mutation­en über die Verschiebung des Leserasters in einem #Protein-codierenden #Gen. Auf welche Weise Frame-Editoren aus Polymerase und #Cas9-Nuklease sie künftig korrigieren könnten, beschreibt @[email protected]: 👉 https://www.laborjournal.de/editorials/2664.php

Target site preferences mean that no single #Cas9 can edit all sequences. Study identifies PI domain variants in natural SaCas9 orthologs that recognize a simple NNGG PAM; these can confer NNGG PAM recognition, expanding the #CRISPR repertoire #PLOSBiology https://plos.io/3gJB4OR

Target site preferences mean that no single #Cas9 can edit all sequences. Study identifies PI domain variants in natural SaCas9 orthologs that recognize a simple NNGG PAM; these can confer NNGG PAM recognition, expanding the #CRISPR repertoire #PLOSBiology https://plos.io/3gJB4OR

Target site preferences mean that no single #Cas9 can edit all sequences. Study identifies PI domain variants in natural SaCas9 orthologs that recognize a simple NNGG PAM; these can confer NNGG PAM recognition, expanding the #CRISPR repertoire #PLOSBiology https://plos.io/3gJB4OR