#2p — Public Fediverse posts

🧠New paper by Carneiro-Nascimento et al: awake #invivo #2p #CalciumImaging reveals diverse #CalciumDynamics in #meningeal #macrophages during cortical spreading depolarization (CSD), an aberrant #brain #hyperexcitability event linked to #migraine, #stroke, and #traumatic brain injury. #Neuroscience

Intravital calcium imaging of ...

🧠New paper by Carneiro-Nascimento et al: awake #invivo #2p #CalciumImaging reveals diverse #CalciumDynamics in #meningeal #macrophages during cortical spreading depolarization (CSD), an aberrant #brain #hyperexcitability event linked to #migraine, #stroke, and #traumatic brain injury. #Neuroscience

Intravital calcium imaging of ...

🧠New paper by Carneiro-Nascimento et al: awake #invivo #2p #CalciumImaging reveals diverse #CalciumDynamics in #meningeal #macrophages during cortical spreading depolarization (CSD), an aberrant #brain #hyperexcitability event linked to #migraine, #stroke, and #traumatic brain injury. #Neuroscience

Intravital calcium imaging of ...

🧠New paper by Carneiro-Nascimento et al: awake #invivo #2p #CalciumImaging reveals diverse #CalciumDynamics in #meningeal #macrophages during cortical spreading depolarization (CSD), an aberrant #brain #hyperexcitability event linked to #migraine, #stroke, and #traumatic brain injury. #Neuroscience

Intravital calcium imaging of ...

🧠New paper by Carneiro-Nascimento et al: awake #invivo #2p #CalciumImaging reveals diverse #CalciumDynamics in #meningeal #macrophages during cortical spreading depolarization (CSD), an aberrant #brain #hyperexcitability event linked to #migraine, #stroke, and #traumatic brain injury. #Neuroscience

Intravital calcium imaging of ...

🧠 New paper by Carneiro-Nascimento et al: awake #invivo #2p #CalciumImaging reveals diverse #CalciumDynamics in #meningeal #macrophages during cortical spreading depolarization (CSD), an aberrant #brain #hyperexcitability event linked to #migraine, #stroke, and #traumatic brain injury.

📝 https://doi.org/10.7554/eLife.109888

#Neuroimmunology #Neuroscience #imaging @eLife https://fediscience.org/@eLife/116605475951455191

🧠 New paper by Carneiro-Nascimento et al: awake #invivo #2p #CalciumImaging reveals diverse #CalciumDynamics in #meningeal #macrophages during cortical spreading depolarization (CSD), an aberrant #brain #hyperexcitability event linked to #migraine, #stroke, and #traumatic brain injury.

📝 https://doi.org/10.7554/eLife.109888

#Neuroimmunology #Neuroscience #imaging @eLife https://fediscience.org/@eLife/116605475951455191

🧠 New paper by Carneiro-Nascimento et al: awake #invivo #2p #CalciumImaging reveals diverse #CalciumDynamics in #meningeal #macrophages during cortical spreading depolarization (CSD), an aberrant #brain #hyperexcitability event linked to #migraine, #stroke, and #traumatic brain injury.

📝 https://doi.org/10.7554/eLife.109888

#Neuroimmunology #Neuroscience #imaging @eLife https://fediscience.org/@eLife/116605475951455191

🧠 New paper by Carneiro-Nascimento et al: awake #invivo #2p #CalciumImaging reveals diverse #CalciumDynamics in #meningeal #macrophages during cortical spreading depolarization (CSD), an aberrant #brain #hyperexcitability event linked to #migraine, #stroke, and #traumatic brain injury.

📝 https://doi.org/10.7554/eLife.109888

#Neuroimmunology #Neuroscience #imaging @eLife https://fediscience.org/@eLife/116605475951455191

🧠 New paper by Carneiro-Nascimento et al: awake #invivo #2p #CalciumImaging reveals diverse #CalciumDynamics in #meningeal #macrophages during cortical spreading depolarization (CSD), an aberrant #brain #hyperexcitability event linked to #migraine, #stroke, and #traumatic brain injury.

📝 https://doi.org/10.7554/eLife.109888

#Neuroimmunology #Neuroscience #imaging @eLife https://fediscience.org/@eLife/116605475951455191

🐭🎶 New paper by Isko et al, who study #Alston’s singing mouse, a rodent with loud, stereotyped songs.

Using bulk tracing, serial #2P #tomography, & #MAPseq of >76,000 barcoded neurons, they find expanded projections from #orofacial #MotorCortex to #auditory #cortex & #PAG. This suggests that singing / #Vocalization may emerge not from entirely new circuits, but from selective strengthening of existing motor, auditory & vocal-control pathways.

📝 https://doi.org/10.1038/s41586-026-10458-y

#Neuroscience #Evolution

🐭🎶 New paper by Isko et al, who study #Alston’s singing mouse, a rodent with loud, stereotyped songs.

Using bulk tracing, serial #2P #tomography, & #MAPseq of >76,000 barcoded neurons, they find expanded projections from #orofacial #MotorCortex to #auditory #cortex & #PAG. This suggests that singing / #Vocalization may emerge not from entirely new circuits, but from selective strengthening of existing motor, auditory & vocal-control pathways.

📝 https://doi.org/10.1038/s41586-026-10458-y

#Neuroscience #Evolution

🐭🎶 New paper by Isko et al, who study #Alston’s singing mouse, a rodent with loud, stereotyped songs.

Using bulk tracing, serial #2P #tomography, & #MAPseq of >76,000 barcoded neurons, they find expanded projections from #orofacial #MotorCortex to #auditory #cortex & #PAG. This suggests that singing / #Vocalization may emerge not from entirely new circuits, but from selective strengthening of existing motor, auditory & vocal-control pathways.

📝 https://doi.org/10.1038/s41586-026-10458-y

#Neuroscience #Evolution

🐭🎶 New paper by Isko et al, who study #Alston’s singing mouse, a rodent with loud, stereotyped songs.

Using bulk tracing, serial #2P #tomography, & #MAPseq of >76,000 barcoded neurons, they find expanded projections from #orofacial #MotorCortex to #auditory #cortex & #PAG. This suggests that singing / #Vocalization may emerge not from entirely new circuits, but from selective strengthening of existing motor, auditory & vocal-control pathways.

📝 https://doi.org/10.1038/s41586-026-10458-y

#Neuroscience #Evolution

🐭🎶 New paper by Isko et al, who study #Alston’s singing mouse, a rodent with loud, stereotyped songs.

Using bulk tracing, serial #2P #tomography, & #MAPseq of >76,000 barcoded neurons, they find expanded projections from #orofacial #MotorCortex to #auditory #cortex & #PAG. This suggests that singing / #Vocalization may emerge not from entirely new circuits, but from selective strengthening of existing motor, auditory & vocal-control pathways.

📝 https://doi.org/10.1038/s41586-026-10458-y

#Neuroscience #Evolution

🔬 New #SpectralUnmixing paper by Ashesh et al. ( @florianjug): A #DeepLearning-based framework #MicroSplit for #FluorescenceMicroscopy that separates highly overlapping #fluorophore signals directly from multiplexed #ImagingData. Improves signal separation, reduces crosstalk, & enables more accurate multi-channel #imaging w/o requiring extensive reference measurements. Can’t wait to try this out on our in vivo #2P/ #3P imaging data:

📝 https://doi.org/10.1038/s41592-026-03082-1

#Bioimaging

🔬 New #SpectralUnmixing paper by Ashesh et al. ( @florianjug): A #DeepLearning-based framework #MicroSplit for #FluorescenceMicroscopy that separates highly overlapping #fluorophore signals directly from multiplexed #ImagingData. Improves signal separation, reduces crosstalk, & enables more accurate multi-channel #imaging w/o requiring extensive reference measurements. Can’t wait to try this out on our in vivo #2P/ #3P imaging data:

📝 https://doi.org/10.1038/s41592-026-03082-1

#Bioimaging

🔬 New #SpectralUnmixing paper by Ashesh et al. ( @florianjug): A #DeepLearning-based framework #MicroSplit for #FluorescenceMicroscopy that separates highly overlapping #fluorophore signals directly from multiplexed #ImagingData. Improves signal separation, reduces crosstalk, & enables more accurate multi-channel #imaging w/o requiring extensive reference measurements. Can’t wait to try this out on our in vivo #2P/ #3P imaging data:

📝 https://doi.org/10.1038/s41592-026-03082-1

#Bioimaging

🔬 New #SpectralUnmixing paper by Ashesh et al. ( @florianjug): A #DeepLearning-based framework #MicroSplit for #FluorescenceMicroscopy that separates highly overlapping #fluorophore signals directly from multiplexed #ImagingData. Improves signal separation, reduces crosstalk, & enables more accurate multi-channel #imaging w/o requiring extensive reference measurements. Can’t wait to try this out on our in vivo #2P/ #3P imaging data:

📝 https://doi.org/10.1038/s41592-026-03082-1

#Bioimaging

🔬 New #SpectralUnmixing paper by Ashesh et al. ( @florianjug): A #DeepLearning-based framework #MicroSplit for #FluorescenceMicroscopy that separates highly overlapping #fluorophore signals directly from multiplexed #ImagingData. Improves signal separation, reduces crosstalk, & enables more accurate multi-channel #imaging w/o requiring extensive reference measurements. Can’t wait to try this out on our in vivo #2P/ #3P imaging data:

📝 https://doi.org/10.1038/s41592-026-03082-1

#Bioimaging

#2P #Xploria online: Diagnosegestützt fördern in #Deutsch und #DaZ

Freischalten, Lernstände einsehen, Aufträge zuweisen – erklären und sofort praktisch umgesetzt.
Plus: Umgang mit Schüler-Logins und Austausch mit 2P-Expertinnen und Experten

Digitale Infoveranstaltung zu 2P Sekundar – Lerntool Xploria Deutsch und DaZ am 20. Mai 2026 von 13:30 bis 17:30 Uhr

There‘s now a #PyTorch version of #Cascade 👌, the #spike inference pipeline for #Calcium #imaging, as @ptrrupprecht just explained on his blog:

🌍 https://gcamp6f.com/2026/02/17/cascadetorch-a-pytorch-version-of-cascade-for-spike-inference/
🧑‍💻 https://github.com/PTRRupprecht/CascadeTorch
📄 https://www.nature.com/articles/s41593-021-00895-5

#CascadeTorch #DeepLearning #CompNeuro #Neuroscience #2pImaging #2p #opensource

There‘s now a #PyTorch version of #Cascade 👌, the #spike inference pipeline for #Calcium #imaging, as @ptrrupprecht just explained on his blog:

🌍 https://gcamp6f.com/2026/02/17/cascadetorch-a-pytorch-version-of-cascade-for-spike-inference/
🧑‍💻 https://github.com/PTRRupprecht/CascadeTorch
📄 https://www.nature.com/articles/s41593-021-00895-5

#CascadeTorch #DeepLearning #CompNeuro #Neuroscience #2pImaging #2p #opensource

There‘s now a #PyTorch version of #Cascade 👌, the #spike inference pipeline for #Calcium #imaging, as @ptrrupprecht just explained on his blog:

🌍 https://gcamp6f.com/2026/02/17/cascadetorch-a-pytorch-version-of-cascade-for-spike-inference/
🧑‍💻 https://github.com/PTRRupprecht/CascadeTorch
📄 https://www.nature.com/articles/s41593-021-00895-5

#CascadeTorch #DeepLearning #CompNeuro #Neuroscience #2pImaging #2p #opensource

There‘s now a #PyTorch version of #Cascade 👌, the #spike inference pipeline for #Calcium #imaging, as @ptrrupprecht just explained on his blog:

🌍 https://gcamp6f.com/2026/02/17/cascadetorch-a-pytorch-version-of-cascade-for-spike-inference/
🧑‍💻 https://github.com/PTRRupprecht/CascadeTorch
📄 https://www.nature.com/articles/s41593-021-00895-5

#CascadeTorch #DeepLearning #CompNeuro #Neuroscience #2pImaging #2p #opensource

There‘s now a #PyTorch version of #Cascade 👌, the #spike inference pipeline for #Calcium #imaging, as @ptrrupprecht just explained on his blog:

🌍 https://gcamp6f.com/2026/02/17/cascadetorch-a-pytorch-version-of-cascade-for-spike-inference/
🧑‍💻 https://github.com/PTRRupprecht/CascadeTorch
📄 https://www.nature.com/articles/s41593-021-00895-5

#CascadeTorch #DeepLearning #CompNeuro #Neuroscience #2pImaging #2p #opensource

Awesome! #Suite2p by @computingnature et al. has been pushed to version 1.0 👏 It now supports GPU computation:

📄 https://www.biorxiv.org/content/10.64898/2026.02.04.703741v1
🧑‍💻 https://github.com/MouseLand/suite2p

#Neuroscience #Imaging #2p #2pImaging

Black Hole (DataBioTics) for the TI-99/4A

https://youtu.be/qTshqxv_hqU?si=Ds2pp3UuI8JoLXH0

#BlackHole #TI994A #Action #Shooter #TwoPlayerGame #2PlayerGame #2P #TwoPlayer #2Player #DataBioTics

Black Hole (DataBioTics) for the TI-99/4A

https://youtu.be/qTshqxv_hqU?si=Ds2pp3UuI8JoLXH0

#BlackHole #TI994A #Action #Shooter #TwoPlayerGame #2PlayerGame #2P #TwoPlayer #2Player #DataBioTics

Beyond Parsec for the TI-99/4A

https://youtu.be/T2vQb5vKu_4?si=tW5RwfjDRcZyOYfo

#BeyondParsec #TI994A #Action #Shooter #2PlayerOnly #2Player #TwoPlayerOnly #TwoPlayerOnly #2P #DataBioTicsInc #DataBioTics

Beyond Parsec for the TI-99/4A

https://youtu.be/T2vQb5vKu_4?si=tW5RwfjDRcZyOYfo

#BeyondParsec #TI994A #Action #Shooter #2PlayerOnly #2Player #TwoPlayerOnly #TwoPlayerOnly #2P #DataBioTicsInc #DataBioTics

🧠 New preprint by Kim et al. (2025) from David Anderson’s lab: A line #attractor maintains aggressiveness during feeding in “hangry” mice 🍔🐁. Using in vivo #CalciumImaging and #rSLDS modeling, they show how moderate fasting stabilizes an aggression-related attractor in #VMHvl, while prolonged fasting collapses it, linking hunger, motivation, and aggression through #PopulationDynamics:

🌍 https://doi.org/10.1101/2025.10.16.682711

#Neuroscience #CompNeuro #Behavior #AttractorDynamics #Hypothalamus #2p #imaging

🧠 Rao & Ballard’s (1999) spatial #PredictiveCoding theory gets strong support: Zhang et al. (2025) show in mouse #VisualCortex that predictive coding is primarily spatial, not temporal. #2P #imaging of ~20,000 neurons found mismatch responses only when new spatial landmarks appeared, but not when sequences were reordered:

🌍 https://doi.org/10.1101/2025.09.17.676794

#Neuroscience

🧠 Rao & Ballard’s (1999) spatial #PredictiveCoding theory gets strong support: Zhang et al. (2025) show in mouse #VisualCortex that predictive coding is primarily spatial, not temporal. #2P #imaging of ~20,000 neurons found mismatch responses only when new spatial landmarks appeared, but not when sequences were reordered:

🌍 https://doi.org/10.1101/2025.09.17.676794

#Neuroscience

🧠 Rao & Ballard’s (1999) spatial #PredictiveCoding theory gets strong support: Zhang et al. (2025) show in mouse #VisualCortex that predictive coding is primarily spatial, not temporal. #2P #imaging of ~20,000 neurons found mismatch responses only when new spatial landmarks appeared, but not when sequences were reordered:

🌍 https://doi.org/10.1101/2025.09.17.676794

#Neuroscience

🧠 Rao & Ballard’s (1999) spatial #PredictiveCoding theory gets strong support: Zhang et al. (2025) show in mouse #VisualCortex that predictive coding is primarily spatial, not temporal. #2P #imaging of ~20,000 neurons found mismatch responses only when new spatial landmarks appeared, but not when sequences were reordered:

🌍 https://doi.org/10.1101/2025.09.17.676794

#Neuroscience

🧠 Rao & Ballard’s (1999) spatial #PredictiveCoding theory gets strong support: Zhang et al. (2025) show in mouse #VisualCortex that predictive coding is primarily spatial, not temporal. #2P #imaging of ~20,000 neurons found mismatch responses only when new spatial landmarks appeared, but not when sequences were reordered:

🌍 https://doi.org/10.1101/2025.09.17.676794

#Neuroscience

Post-doc in network mechanisms of long-term memory and aging

University of Connecticut Health Center

Post-doc in #2p, #electrophysiology and closed-loop #optogenetics of hippocampal network mechanisms of long-term #memory

See the full job description on jobRxiv: https://jobrxiv.org/job/university-of-connecticut-health-center-27778-post-doc-in-ne...
https://jobrxiv.org/job/university-of-connecticut-health-center-27778-post-doc-in-network-mechanisms-of-long-term-memory-and-aging/?feed_id=89779

Post-doc in network mechanisms of long-term memory and aging

University of Connecticut Health Center

Post-doc in #2p, #electrophysiology and closed-loop #optogenetics of hippocampal network mechanisms of long-term #memory

See the full job description on jobRxiv: https://jobrxiv.org/job/university-of-connecticut-health-center-27778-post-doc-in-ne...
https://jobrxiv.org/job/university-of-connecticut-health-center-27778-post-doc-in-network-mechanisms-of-long-term-memory-and-aging/?feed_id=89408

Our new #preprint on #ThreePhoton in vivo #imaging of #neurons and #glia 🔬 in the medial prefrontal cortex (#mPFC) 🧠 with sub-cellular resolution 🔍 is out! Check it out on #biorxiv:

🌍 https://www.biorxiv.org/content/10.1101/2024.08.28.610026v1

#Neuroscience #multiphoton #3p vs. #2p #twophoton #hippocampus #drosophila #MouseModel #DeepImaging #preprintalert #DZNE @dzne

Our new #preprint on #ThreePhoton in vivo #imaging of #neurons and #glia 🔬 in the medial prefrontal cortex (#mPFC) 🧠 with sub-cellular resolution 🔍 is out! Check it out on #biorxiv:

🌍 https://www.biorxiv.org/content/10.1101/2024.08.28.610026v1

#Neuroscience #multiphoton #3p vs. #2p #twophoton #hippocampus #drosophila #MouseModel #DeepImaging #preprintalert #DZNE @dzne

Our new #preprint on #ThreePhoton in vivo #imaging of #neurons and #glia 🔬 in the medial prefrontal cortex (#mPFC) 🧠 with sub-cellular resolution 🔍 is out! Check it out on #biorxiv:

🌍 https://www.biorxiv.org/content/10.1101/2024.08.28.610026v1

#Neuroscience #multiphoton #3p vs. #2p #twophoton #hippocampus #drosophila #MouseModel #DeepImaging #preprintalert #DZNE @dzne

Our new #preprint on #ThreePhoton in vivo #imaging of #neurons and #glia 🔬 in the medial prefrontal cortex (#mPFC) 🧠 with sub-cellular resolution 🔍 is out! Check it out on #biorxiv:

🌍 https://www.biorxiv.org/content/10.1101/2024.08.28.610026v1

#Neuroscience #multiphoton #3p vs. #2p #twophoton #hippocampus #drosophila #MouseModel #DeepImaging #preprintalert #DZNE @dzne

Our new #preprint on #ThreePhoton in vivo #imaging of #neurons and #glia 🔬 in the medial prefrontal cortex (#mPFC) 🧠 with sub-cellular resolution 🔍 is out! Check it out on #biorxiv:

🌍 https://www.biorxiv.org/content/10.1101/2024.08.28.610026v1

#Neuroscience #multiphoton #3p vs. #2p #twophoton #hippocampus #drosophila #MouseModel #DeepImaging #preprintalert #DZNE @dzne

Overdrive for the ZX81

https://www.youtube.com/watch?v=2r85_0x6y38

#Overdrive #ZX81 #2Player #TwoPlayer #2P #TP #PeterDavis #DavidButtery

Overdrive for the ZX81

https://www.youtube.com/watch?v=2r85_0x6y38

#Overdrive #ZX81 #2Player #TwoPlayer #2P #TP #PeterDavis #DavidButtery

Videofilm neues #Lerntool #Deutsch #2P #Xploria

Mit der Weiterentwicklung von 2P können Schüler*innen (5.-10.) aller Schularten Lerninhalte auf Grundlage der vorangegangenen Diagnostik in einer motivierenden Umgebung üben und ihre Kompetenzen vertiefen.

Die Lernaufgaben werden im Kontext eines narrativen Rahmens "Reise um die Welt" dargeboten.
2P Xploria befindet sich derzeit in der Pilotierung und wird ab Herbst 2024 den Schulen im Regelbetrieb zur Verfügung stehen.

https://kompetenzfeststellung.bildung-rp.de/2p-potenzial-perspektive/2p-xploria-lerntool-deutsch.html

Ooh! Last Bug Standing by @StuffByBez from Surprised Stare Games arrived! Thank you for the review copy. 😃👍 #boardgames #TwoPlayerGames #2p #reviews

Ooh! Last Bug Standing by @StuffByBez from Surprised Stare Games arrived! Thank you for the review copy. 😃👍 #boardgames #TwoPlayerGames #2p #reviews

Ooh! Last Bug Standing by @StuffByBez from Surprised Stare Games arrived! Thank you for the review copy. 😃👍 #boardgames #TwoPlayerGames #2p #reviews